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Cationised gelatin and hyaluronic acid coating enhances polyethylene terephthalate artificial ligament graft osseointegration in porcine bone tunnels

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Abstract

Purpose

The aim of this study was to investigate whether cationised gelatin and hyaluronic acid (CH) coating could induce polyethylene terephthalate (PET) artificial ligament graft osseointegration in the bone tunnel.

Methods

Surface modification of PET artificial ligament graft was performed by layer-by-layer (LBL) self-assembly CH coating. Six pigs underwent anterior cruciate ligament (ACL) reconstruction on the right knees, with three pigs receiving the CH-coated PET grafts and the other three pigs non-CH-coated PET grafts as controls. They were sacrificed at three months after surgery and the graft-bone complexes were acquired for computed tomography (CT) scan and histological examination.

Results

CT scans showed a significant difference at the distal femoral site (p = 0.031) or at the distal tibial site (p = 0.0078), but no significant difference in the bone tunnel areas’ enlargement at other sites (p > 0.05) between the CH group and the control group. Histologically, application of CH coating induced new bone formation between graft and bone at three months compared with the controls at the distal site. The interface width of the CH group was significantly lower than that of the control group at the distal femoral site (p = 0.0327) and at the distal tibial site (p = 0.0047).

Conclusions

The study has shown that CH coating on the PET artificial ligament surface has a positive biological effect in the induction of artificial ligament osseointegration within the bone tunnel at the distal site of the bone tunnel.

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Acknowledgments

This work was supported by the Grants from 973 Project from the Ministry of Science and Technology of China (No. 2009CB930000), the Young Project of National Natural Science Foundation of China (81000816) and the Project of Shanghai Municipal Science and Technology Commission (11JC1401700).

Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Sports Medicine, Huashan Hospital, No 12, Wulumuqi Zhong Road, Shanghai, 200040, People’s Republic of China

    Samson Cho, Hong Li, Jia Jiang & Shiyi Chen

  2. Department of Orthopaedics, Zhongshan Hospital, Fudan University, Shanghai, People’s Republic of China

    Chen Chen

  3. Department of Radiology, Huashan Hospital, Shanghai, People’s Republic of China

    Hongyue Tao

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  1. Samson Cho
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  2. Hong Li
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  3. Chen Chen
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  4. Jia Jiang
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Correspondence to Shiyi Chen.

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Cho, S., Li, H., Chen, C. et al. Cationised gelatin and hyaluronic acid coating enhances polyethylene terephthalate artificial ligament graft osseointegration in porcine bone tunnels. International Orthopaedics (SICOT) 37, 507–513 (2013). https://doi.org/10.1007/s00264-012-1694-3

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  • DOI: https://doi.org/10.1007/s00264-012-1694-3

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