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Boophilus microplus Infection by Beauveria amorpha andBeauveria bassiana: SEM Analysis and Regulation of Subtilisin-like Proteases and Chitinases

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Abstract

Beauveria bassiana is a well-known broad-range arthropod pathogen which has been used in biological control of several pest insects and ticks such as Boophilus microplus. Beauveria amorpha has both endophytic and entomopathogenic characteristics, but its capacity for biological control has still not been studied. During the processes of host infection, B. bassiana and B. amorpha produce several hydrolytic extracellular enzymes, including proteases and chitinases, which probably degrade the host cuticle and are suggested to be pathogenicity determinants. To access the role of these enzymes during infection in the tick B. microplus, we analyzed their secretion during fungus growth in single and combined carbon sources, compared to complex substrates such as chitin and B. microplus cuticle. Chitin and tick cuticle-induced chitinase in both fungus and protease was induced only by tick cuticle. SEM analysis of B. amorpha and B. bassiana infecting B. microplus showed apressorium formation during penetration on cattle tick cuticle.

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Acknowledgments

This work was supported by grants and fellowships from FAPERGS, CNPq, CAPES, and PADCT. We thank Dr. Ida Chapaval Pimentel for B. amorpha endophytic isolates.

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Authors and Affiliations

  1. Instituto de Biotecnologia, Universidade de Caxias do Sul, Caxias do Sul, RS, Brazil

    Roberta Alvares Campos, Juliano Tomazzoni Boldo, Neiva Monteiro de Barros & João Lúcio de Azevedo

  2. Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, P.O. Box 15005, 90540-000, Porto Alegre, RS, Brazil

    Walquíria Arruda, Márcia Vanusa da Silva, Augusto Schrank & Marilene Henning Vainstein

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  1. Roberta Alvares Campos
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  2. Walquíria Arruda
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  8. Marilene Henning Vainstein
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Correspondence to Marilene Henning Vainstein.

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Campos, R.A., Arruda, W., Boldo, J.T. et al. Boophilus microplus Infection by Beauveria amorpha andBeauveria bassiana: SEM Analysis and Regulation of Subtilisin-like Proteases and Chitinases. Curr Microbiol 50, 257–261 (2005). https://doi.org/10.1007/s00284-004-4460-y

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