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Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus Attachment Patterns on Glass Surfaces with Nanoscale Roughness

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Abstract

Attachment tendencies of Escherichia coli K12, Pseudomonas aeruginosa ATCC 9027, and Staphylococcus aureus CIP 68.5 onto glass surfaces of different degrees of nanometer-scale roughness have been studied. Contact-angle and surface-charge measurements, atomic force microscopy (AFM), scanning electron microscopy (SEM), and confocal laser scanning microscopy (CLSM) were employed to characterize substrata and bacterial surfaces. Modification of the glass surface resulted in nanometer-scale changes in the surface topography, whereas the physicochemical characteristics of the surfaces remained almost constant. AFM analysis indicated that the overall surface roughness parameters were reduced by 60–70%. SEM, CLSM, and AFM analysis clearly demonstrates that although E. coli, P. aeruginosa and S. aureus present significantly different patterns of attachment, all of the species exhibited a greater propensity for adhesion to the “nano-smooth” surface. The bacteria responded to the surface modification with a remarkable change in cellular metabolic activity, as shown by the characteristic cell morphologies, production of extracellular polymeric substances, and an increase in the number of bacterial cells undergoing attachment.

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Acknowledgments

This study was supported in part by Australian Research Council (ARC).

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Authors and Affiliations

  1. Faculty of Life and Social Sciences, Swinburne University of Technology, PO Box 218, Hawthorn, Victoria, 3122, Australia

    Natasa Mitik-Dineva, Vi Khanh Truong, Francois Malherbe, Russell J. Crawford & Elena P. Ivanova

  2. Center for Atom Optics and Ultrafast Spectroscopy, Swinburne University of Technology, PO Box 218, Hawthorn, Victoria, 3122, Australia

    James Wang & Paul Stoddart

Authors
  1. Natasa Mitik-Dineva
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  2. James Wang
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  3. Vi Khanh Truong
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  4. Paul Stoddart
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  5. Francois Malherbe
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  6. Russell J. Crawford
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  7. Elena P. Ivanova
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Correspondence to Elena P. Ivanova.

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Mitik-Dineva, N., Wang, J., Truong, V.K. et al. Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus Attachment Patterns on Glass Surfaces with Nanoscale Roughness. Curr Microbiol 58, 268–273 (2009). https://doi.org/10.1007/s00284-008-9320-8

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  • DOI: https://doi.org/10.1007/s00284-008-9320-8

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