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Characterization of the Mineral Phosphate-Solubilizing Activity of Pantoea aglomerans MMB051 Isolated from an Iron-Rich Soil in Southeastern Venezuela (Bolívar State)

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Abstract

The mineral phosphate-solubilizing (MPS) activity of a Pantoea agglomerans strain, namely MMB051, isolated from an iron-rich, acidic soil near Ciudad Piar (Bolívar State, Venezuela), was characterized on a chemically defined medium (NBRIP). Various insoluble inorganic phosphates, including tri-calcium phosphate [Ca3(PO4)2], iron phosphate (FePO4), aluminum phosphate (AlPO4), and Rock Phosphate (RP) were tested as sole sources of P for bacterial growth. Solubilization of Ca3(PO4)2 was very efficient and depended on acidification of the external milieu when MMB051 cells were grown in the presence of glucose. This was also the case when RP was used as the sole P source. On the other hand, the solubilization efficiency toward more insoluble mineral phosphates (FePO4 and AlPO4) was shown to be very low. Even though gluconic acid (GA) was detected on culture supernatants of strain MMB051, a consequence of the direct oxidation pathway of glucose, inorganic-P solubilization seemed also to be related to other processes dependent on active cell growth. Among these, proton release by ammonium (NH +4 ) fixation appeared to be of paramount importance to explain inorganic-P solubilization mediated by strain MMB051. On the contrary, the presence of nitrate (NO –3 ) salts as the sole N source affected negatively the ability of MMB051 cells to solubilize inorganic P.

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Acknowledgments

This work was financed by CDCHT-ULA Grant No. 1312-05-03-A. The authors are grateful to Professor Carmelo Rosquete (Facultad de Ciencias, ULA) for his help in TLC procedures. We also thank BIOMI Lab (Facultad de Ciencias, ULA) and Postgrado en Biotecnología de Microorganismos for their collaborative support. EP acknowledges FONACIT (Programa de Formación de Alto Nivel) and the Ministerio de Ciencia y Tecnología of Venezuela for supporting her PhD studies.

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Authors and Affiliations

  1. Laboratorio de Microbiología Molecular y Biotecnología, Facultad de Ciencias, Núcleo de La Hechicera, Universidad de Los Andes, Mérida, 5101, Venezuela

    Miguel Sulbarán, Elizabeth Pérez, María M. Ball & Luis Andrés Yarzábal

  2. Laboratorio de Resonancia Magnética Nuclear, Facultad de Ciencias, Núcleo de La Hechicera, Universidad de Los Andes, Mérida, 5101, Venezuela

    Alí Bahsas

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  1. Miguel Sulbarán
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  2. Elizabeth Pérez
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  3. María M. Ball
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  4. Alí Bahsas
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  5. Luis Andrés Yarzábal
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Correspondence to Luis Andrés Yarzábal.

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Sulbarán, M., Pérez, E., Ball, M.M. et al. Characterization of the Mineral Phosphate-Solubilizing Activity of Pantoea aglomerans MMB051 Isolated from an Iron-Rich Soil in Southeastern Venezuela (Bolívar State). Curr Microbiol 58, 378–383 (2009). https://doi.org/10.1007/s00284-008-9327-1

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  • DOI: https://doi.org/10.1007/s00284-008-9327-1

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