Skip to main content
SpringerLink
Log in

Biocontrol Potential of Soybean Bacterial Endophytes Against Charcoal Rot Fungus, Rhizoctonia bataticola

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

A total of 137 bacterial isolates from surface sterilized root, stem, and nodule tissues of soybean were screened for their antifungal activity against major phytopathogens like Rhizoctonia bataticola, Macrophomina phaseolina, Fusarium udam, and Sclerotium rolfsii. Nine bacterial endophytes suppressed the pathogens under in vitro plate assay. These were characterized biochemically and identified at the genus level based on their partial sequence analysis of 16S rDNA. Eight of the isolates belonged to Bacillus and one to Paenibacillus. The phylogenetic relationship among the selected isolates was studied and phylogenetic trees were generated. The selected isolates were screened for biocontrol traits like production of hydrogen cyanide (HCN), siderophore, hydrolytic enzymes, antibiotics, and plant growth promoting traits like indole 3-acetic acid production, phosphate solubilization, and nitrogen fixation. A modified assessment scheme was used to select the most efficient biocontrol isolates Paenibacillus sp. HKA-15 (HKA-15) and Bacillus sp. HKA-121 (HKA-121) as potential candidates for charcoal rot biocontrol as well as soybean plant growth promotion.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Singh RDN, Kaiser SAKM (1995) Evaluation of some systemic and non systemic fungicides against the charcoal rot pathogen Macrophomina phaseolina of maize. J Trop Agr 33:54–58

    Google Scholar 

  2. Kobayashi D, Palumbo JD (2000) Bacterial endophytes and their effects on plants and uses in agriculture. In: Bacon CW, White JF (eds) Microbial endophytes. Marcel Dekker, New York, pp 199–233

    Google Scholar 

  3. Cao L, Qui Z, You J, Tan H, Zhou S (2005) Isolation and characterization of endophytic streptomycete antagonists of fusarium wilt pathogen from surface sterilized banana roots. FEMS Microbiol Lett 247:147–152

    Article  PubMed  CAS  Google Scholar 

  4. Berg G, Krechel A, Ditz M, Sikora RA, Ulrich A, Hallmann J (2005) Endophytic and ectophytic potato associated bacterial communities differ in structure and antagonistic function against plant pathogenic fungi. FEMS Microbiol Ecol 51:215–229

    Article  PubMed  CAS  Google Scholar 

  5. Sturz AV, Christie BR, Matheson BG, Arsenault WJ, Buchanan NA (1999) Endophytic bacterial communities in the periderm of potato tubers and their potential to improve resistance to soil-borne plant pathogens. Plant Pathol 48:360–369

    Article  Google Scholar 

  6. Hung PQ, Senthilkumar M, Govindasamy V, Annapurna K (2007) Isolation and characterization of endophytic bacteria from wild and cultivated soybean varieties. Biol Fertil Soil 44:155–162

    Article  Google Scholar 

  7. Priest FG, Goodfellow M, Todd C (1981) The genus Bacillus: a numerical analysis. In: Berkeley RCW, Goodfellow M (eds) The aerobic endospore forming bacteria classification and identification. Academic Press, London, pp 315–335

    Google Scholar 

  8. Edwards U, Rogall T, Blöcker H, Emde M, Böttger EC (1989) Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Res 17:7843–7853

    Article  PubMed  CAS  Google Scholar 

  9. Thompson JD, Gibson TJ, PleWniak F, Jeanmougin F, Higgins DG (1997) The clustalx windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4882

    Article  Google Scholar 

  10. Bakker AW, Schipper B (1987) Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomonas spp. mediated plant growth stimulation. Soil Biol Biochem 19:451–457

    CAS  Google Scholar 

  11. Sessitsch A, Reiter B, Berg G (2004) Endophytic bacterial communities of field grown potato plants and their plant growth promoting and antagonistic abilities. Can J Microbiol 50:239–249

    Article  PubMed  CAS  Google Scholar 

  12. Schwyn B, Neiland JB (1987) Universal chemical assay for the detection and determination of Siderophore. Anal Biochem 160:47–56

    Article  PubMed  CAS  Google Scholar 

  13. Raaijmakers JM, Weller DM, Thomashow LS (1997) Frequency of antibiotic producing Pseudomonas sp. in natural environments. Appl Environ Microbiol 63:881–887

    CAS  Google Scholar 

  14. Hurek T, Egener T, Reinhold-Hurek B (1997) Divergence in nitrogenases of Azoarcus spp., proteobacteria of β-subclass. J Bacteriol 179:4172–4178

    PubMed  CAS  Google Scholar 

  15. Gordon AS, Weber RP (1951) Colorimetric estimation of indole acetic acid. Plant Physiol 26:192–195

    Article  PubMed  CAS  Google Scholar 

  16. Mehta S, Nautiyal CS (2001) An efficient method for qualitative screening of phosphate solubilizing bacteria. Curr Microbiol 43:51–56

    Article  PubMed  CAS  Google Scholar 

  17. Bevivino A, Sarrocco S, Dalmastri C, Tabacchioni S, Cantale C, Chiarini L (1998) Characterization of free living maize rhizosphere population of Burkholderia cepacia: effect of seed treatment on disease suppression and growth promotion of maize. FEMS Microbiol Ecol 27:225–237

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors are grateful to the Director, National Research Centre for Soybean, Indore, India, and Dr. Linda S. Thomashow, Washington State University, Pullman, Washington, USA, for providing soybean seed and standard Pseudomonas strains, respectively. The first author thanks the Indian Council of Agricultural Research, India, for his Ph.D. fellowship.

Author information

Authors and Affiliations

  1. Advanced Radiation Technology Institute, Korean Atomic Energy Research Institute, Jeongeup, 580-185, South Korea

    M. Senthilkumar & Young Keun Lee

  2. Division of Crop Physiology, Biochemistry and Microbiology, Indian Institute of Pulse Research, Kanpur, 208024, India

    K. Swarnalakshmi

  3. Division of Microbiology, Indian Agricultural Research Institute, New Delhi, 110012, India

    V. Govindasamy & K. Annapurna

Authors
  1. M. Senthilkumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. Swarnalakshmi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. Govindasamy
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Young Keun Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. K. Annapurna
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. Senthilkumar.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Senthilkumar, M., Swarnalakshmi, K., Govindasamy, V. et al. Biocontrol Potential of Soybean Bacterial Endophytes Against Charcoal Rot Fungus, Rhizoctonia bataticola . Curr Microbiol 58, 288–293 (2009). https://doi.org/10.1007/s00284-008-9329-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00284-008-9329-z

Keywords

Search

Navigation