Skip to main content
SpringerLink
Log in

An Endophytic Sanguinarine-Producing Fungus from Macleaya cordata, Fusarium proliferatum BLH51

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

Fermentation processes using sanguinarine-producing fungi other than Macleaya cordata may be an alternative way to produce sanguinarine (SA), which is a quaternary benzo[c]phenanthridine alkaloid possessing antibacterial, anthelmintic, and anti-inflammatory properties. In this study, a SA-producing endophytic fungus strain BLH51 was isolated from the leaves of M. cordata grown in the Dabie Mountain, China. Strain BLH51 produced SA when grown in potato dextrose liquid medium. The amount of SA produced by this endophytic fungus was quantified to be 178 μg/L by HPLC, substantially lower than that produced by the host tissue. The fungal SA—which was analyzed by thin layer chromatography and high-performance liquid chromatography—was shown to be identical to authentic SA. Strain BLH51 was identified as Fusarium proliferatum based on the morphological characteristics and nuclear ribosomal DNA ITS sequence analysis. To the best of our knowledge, this is the first report concerning the isolation and identification of endophytic SA-producing fungi from the host plant, which further proved that endophytic fungi are valuable reservoirs of bioactive compounds.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Beuria TK, Santra MK, Panda D (2005) Sanguinarine blocks cytokinesis in bacteria by inhibiting FtsZ assembly and bundling. Biochemistry 44:16584–16593

    Article  CAS  PubMed  Google Scholar 

  2. Lovasoa RR, Havet JL, Catherine P, Henri F (2007) Solid-liquid extraction of protopine from Fumaria officinalis L.-Analysis determination, kinetic reaction and model building. Sep Purif Technol 54:253–261

    Article  Google Scholar 

  3. Satou T, Akao N, Matsuhashi R, Koike K, Fujita K, Nikaido T (2002) Inhibitory effect of isoquinoline alkaloids on movement of second-stage larvae of Toxocara canis. Biol Pharm Bull 25:1651–1654

    Article  CAS  PubMed  Google Scholar 

  4. Bai LP, Zhao ZZ, Cai ZW, Jiang ZH (2006) DNA-binding affinities and sequence selectivity of quaternary benzophenanthridine alkaloids sanguinarine, chelerythrine, and nitidine. Bioorg Med Chem 14:5439–5445

    Article  CAS  PubMed  Google Scholar 

  5. Luo XB, Chen B, Yao SZ (2005) Rapid determination of protopine, allocryptopine, sanguinarine and chelerythrine in fruits of Macleaya cordata by microwave-assisted solvent extraction and HPLC-ESI/MS. Phytochem Anal 17:431–438

    Article  Google Scholar 

  6. Saeed SA, Gilani AH, Majoo RU, Shah BH (1997) Anti-thrombotic and anti-inflammatory activities of protopine. Pharmacol Res 36:1–7

    Article  CAS  PubMed  Google Scholar 

  7. Zhou HY, Mineshita S (2000) The effect of berberine chloride on experimental colitis in rats in vivo and in vitro. J Pharmacol Exp Ther 294:822–829

    CAS  PubMed  Google Scholar 

  8. Faddeeva MD, Beliaeva TN (1997) Sanguinarine and ellipticine cytotoxic alkaloids isolated from well-known antitumor plants. Intracellular targets of their action. Tsitologiia 39:181–208

    CAS  PubMed  Google Scholar 

  9. Alcantara J, Bird DA, Franceschi VR, Facchini PJ (2005) Sanguinarine biosynthesis is associated with the endoplasmic reticulum in cultured opium poppy cells after elicitor treatment. Plant Physiol 138:173–183

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  10. Strobel G, Daisy B, Castillo U, Harper J (2004) Natural products from endophytic microorganisms. J Nat Prod 67:257–268

    Article  CAS  PubMed  Google Scholar 

  11. Pirttilä AM, Kamarainen T, Hirsikorpi M, Jaakola L, Hohtola A (2001) DNA isolation methods for medicinal and aromatic plants. Plant Mol Biol Rep 19:273a–273f

    Article  Google Scholar 

  12. White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfland DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, New York

    Google Scholar 

  13. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The Clustal_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  14. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  PubMed  Google Scholar 

  15. Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163

    Article  CAS  PubMed  Google Scholar 

  16. Higgins KL, Arnold AE, Miadlikowska J, Sarvate SD, Lutzoni F (2007) Phylogenetic relationships, host affinity, and geographic structure of boreal and arctic endophytes from three major plant lineages. Mol Phylogenet Evol 42:543–555

    Article  CAS  PubMed  Google Scholar 

  17. Strobel G, Daisy B (2003) Bioprospecting for microbial endophytes and their natural products. Microbiol Mol Biol Rev 67:491–502

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  18. Nirenberg HI, O’Donnell K (1998) New Fusarium species and combinations within the Gibberella fujikuroi species complex. Mycologia 90:434–458

    Article  Google Scholar 

  19. Deng BW, Liu KH, Chen WQ, Ding XW, Xie XC (2009) Fusarium solani, Tax-3, a new endophytic taxol-producing fungus from Taxus chinensis. World J Microbiol Biotechnol 25:139–143

    Article  CAS  Google Scholar 

  20. Marín S, Magan N, Serra J, Ramos AJ, Canela R, Sanchis V (1999) Fumonisin B1 production and growth of Fusarium moniliforme and Fusarium proliferatum on maize, Wheat, and Barley Grain. J Food Sci 64:921–924

    Article  Google Scholar 

  21. Mohana Kumara P, Zuehlke S, Priti V, Ramesha BT, Shweta S, Ravikanth G, Vasudeva R, Santhoshkumar TR, Spiteller M, Uma Shaanker RU (2012) Fusarium proliferatum, an endophytic fungus from Dysoxylum binectariferum Hook.f, produces rohitukine, a chromane alkaloid possessing anti-cancer activity. Antonie Van Leeuwenhoek 101:323–329

    Article  PubMed  Google Scholar 

  22. Cheng ZS, Tang WC, Su ZJ, Cai Y, Sun SF, Chen QJ, Wang FH, Lin YC, She ZG, Vrijmoed LLP (2008) Identification of mangrove endophytic fungus 1403 (Fusarium proliferatum) based on morphological and molecular evidence. J For Res 19:219–224

    Article  Google Scholar 

  23. Jin JM, Baek SR, Lee KR, Lee JK, Yun SH, Kang SC et al (2008) Purification and phytotoxicity of apicidins produced by the Fusarium semitectum KCTC16676. Plant Pathol J 24:417–422

    Article  CAS  Google Scholar 

  24. Germaine K, Keogh E, Garcia-Cabellos G, Borremans B, Barac T, Dowling DN et al (2004) Colonisation of poplar trees by gfp expressing bacterial endophytes. FEMS Microbiol Ecol 48:109–118

    Article  CAS  PubMed  Google Scholar 

  25. Stierle A, Strobel G, Stierle D (1993) Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of pacific yew. Science 260:214–216

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by National Natural Science Foundation of China (Grant No. 31100019) and Anhui Natural Science Research Project of Colleges and Universities of China (Grant Nos. KJ2013A266 and KJ2011Z398). We thank Prof. Yun-jiang Min from College of Biotechnology and Pharmaceutical Engineering, West Anhui University, China for helping with the identification of the plant material. We also wish to thank the anonymous reviewers for constructive comments.

Author information

Authors and Affiliations

  1. College of Biotechnology and Pharmaceutical Engineering, West Anhui University, Lu’an, 237012, China

    Xue-Jun Wang, Chang-Li Min & Rui-Hua Zuo

  2. Research Center for Endophytic Fungi Resources of Dabie Mountain, West Anhui University, Lu’an, 237012, China

    Xue-Jun Wang & Chang-Li Min

  3. Shanghai Laiyi Center of Biopharmaceutical R&D, Shanghai, 201203, China

    Mei Ge

Authors
  1. Xue-Jun Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chang-Li Min
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mei Ge
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Rui-Hua Zuo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Chang-Li Min.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, XJ., Min, CL., Ge, M. et al. An Endophytic Sanguinarine-Producing Fungus from Macleaya cordata, Fusarium proliferatum BLH51. Curr Microbiol 68, 336–341 (2014). https://doi.org/10.1007/s00284-013-0482-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00284-013-0482-7

Keywords

Search

Navigation