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A Bilobalide-Producing Endophytic Fungus, Pestalotiopsis uvicola from Medicinal Plant Ginkgo biloba

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Abstract

For screening bilobalide (BB)-producing endophytic fungi from medicinal plant Ginkgo biloba, a total of 57 fungal isolates were isolated from the internal stem, root, leaf, and bark of the plant G. biloba. Fermentation processes using BB-producing fungi other than G. biloba may become a novel way to produce BB, which is a terpene trilactones exhibiting neuroprotective effects. In this study, a BB-producing endophytic fungal strain GZUYX13 was isolated from the leaves of G. biloba grown in the campus of Guizhou University, Guiyang city, Guizhou province, China. The strain produced BB when grown in potato dextrose liquid medium. The amount of BB produced by this endophytic fungus was quantified to be 106 μg/L via high-performance liquid chromatography (HPLC), substantially lower than that produced by the host tissue. The fungal BB which was analyzed by thin layer chromatography (TLC) and HPLC was proven to be identical to authentic BB. The strain GZUYX13 was identified as Pestalotiopsis uvicola via morphology and ITS rDNA phylogeny. To the best of our knowledge, this is the first report concerning the isolation and identification of endophytic BB-producing Pestalotiopsis spp. from the host plant, which further proved that endophytic fungi have the potential to produce bioactive compounds.

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References

  1. Ahlemeyer B, Mowes A, Krieglstein J (1999) Inhibition of serum deprivation- and staurosporine-induced neuronal apoptosis by Ginkgo biloba extract and some of its constituents. Eur J Pharmacol 367:423–430

    Article  CAS  PubMed  Google Scholar 

  2. Bissett J (1982) Pestalotiopsis menezesiana on greenhouse plantings of Cissus rhombifolia with notes on related fungi occurring on Vitaceae. Can J Bot 60:2570–2574

    Article  Google Scholar 

  3. Chen P, Ozcan M, Harnly J (2007) Chromatographic fingerprint analysis for evaluation of Ginkgo biloba products. Anal Bioanal Chem 389:251–261

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Cui YN, Yi DW, Bai XF, Sun BS, Zhao YQ, Zhang YX (2012) Ginkgolide B produced endophytic fungus (Fusarium oxysporum) isolated from Ginkgo biloba. Fitoterapia 83:913–920

    Article  CAS  PubMed  Google Scholar 

  5. Etienne A, Hecquet F, Soulard C, Spinnewyn B, Clostre F, Braquet P (1986) In vivo inhibition of plasma protein leakage and Salmonella enteritidis-induced mortality in the rat by a specific paf-acether antagonist: BN 52021. Agents Actions 54:368–370

    Article  Google Scholar 

  6. Germaine K, Keogh E, Garcia-Cabellos G, Borremans B, Barac T, Dowling DN et al (2004) Colonisation of poplar trees by gfp expressing bacterial endophytes. FEMS Microbiol Ecol 48:109–118

    Article  CAS  PubMed  Google Scholar 

  7. Higgins KL, Arnold AE, Miadlikowska J, Sarvate SD, Lutzoni F (2007) Phylogenetic relationships, host affinity, and geographic structure of boreal and arctic endophytes from three major plant lineages. Mol Phylogenet Evol 42:543–555

    Article  CAS  PubMed  Google Scholar 

  8. Huang SH, Duke RK, Chebib M, Sasaki K, Wada K, Johnston GA (2003) Bilobalide, a sesquiterpene trilactone from Ginkgo biloba, is an antagonist at recombinant alpha1 beta2 gamma2 L GABA (A) receptors. Eur J Pharmacol 464:1–8

    Article  CAS  PubMed  Google Scholar 

  9. Janssens D, Michiels C, Delaive E, Eliaers F, Drieu K, Remacle J (1995) Protection of hypoxia-induced ATP decrease in endothelial cells by Ginkgo biloba extract and bilobalide. Biochem Pharmacol 50:991–999

    Article  CAS  PubMed  Google Scholar 

  10. Klein J, Chatterjee SS, Loffelholz K (1997) Phospholipid breakdown and choline release under hypoxic conditions: inhibition by bilobalide, a constituent of Ginkgo biloba. Brain Res 755:347–350

    Article  CAS  PubMed  Google Scholar 

  11. Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163

    Article  CAS  PubMed  Google Scholar 

  12. Laurain D, Tremouillaux-Guillerm J, Chenieux JC, van Beek TA (1997) Production of ginkgolide and bilobalide in transformed and gametophyte derived cell cultures of Ginkgo biloba. Phytochemistry 46:127–130

    Article  CAS  Google Scholar 

  13. Maharachchikumbura SSN, Guo LD, Chukeatirote E, Bahkali AH, Hyde KD (2011) Pestalotiopsis—morphology, phylogeny, biochemistry and diversity. Fungal Diver 50:167–187

    Article  Google Scholar 

  14. Michael TC, David KJ, Jeffrey LG (1993) Synthetic studies on the Ginkgolides: total synthesis of (±)-bilobalide. J Am Chem Soc 115:3146–3155

    Article  Google Scholar 

  15. Qin JC, Gao JM, Zhang YM, Yang SX, Bai MS, Ma YT, Laatsch H (2009) Polyhydroxylated steroids from an endophytic fungus, Chaetomiumglobosum ZY-22 isolated from Ginkgo biloba. Steroids 74:786–790

    Article  CAS  PubMed  Google Scholar 

  16. Qin JC, Zhang YM, Hua L, Ma YT, Gao JM (2009) Cytotoxic metabolites produced by Alternaria No.28, an endophytic fungus isolated from Ginkgo biloba. Nat Prod Commun 4:1473–1476

    CAS  PubMed  Google Scholar 

  17. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  PubMed  Google Scholar 

  18. Stierle A, Strobel G, Stierle D (1993) Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of pacific yew. Science 260:214–216

    Article  CAS  PubMed  Google Scholar 

  19. Strobel G, Daisy B (2003) Bioprospecting for microbial endophytes and their natural products. Microbiol Mol Biol Rev 67:491–502

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The Clustal_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. vanBeek TA (2005) Ginkgolides and bilobalide: their physical, chromatographic and spectroscopic properties. Bioorg Med Chem 13:5001–5012

    Article  CAS  Google Scholar 

  22. Wang MX, Chen SL, Yan SZ, Huo J (2003) A preliminary study on the isolation, identification and media of an endophytic fungus producing flavones. J Nanjing Nor Univ (Nat Sci) 26:106–110

    CAS  Google Scholar 

  23. White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfland DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, New York

    Google Scholar 

  24. Xu J, Ebada SS, Proksch P (2010) Pestalotiopsis a highly creative genus: chemistry and bioactivity of secondary metabolites. Fungal Diver 44:15–31

    Article  Google Scholar 

  25. Xu J, Yang XB, Lin Q (2014) Chemistry and biology of Pestalotiopsis-derived natural products. Fungal Diver 66:37–68

    Article  Google Scholar 

  26. Yi DW, Zhang YX, He JY, Zhao WQ, Wu CF (2007) Fermentation metabolites of endophytic fungus isolated from Ginkgo biloba. J Microbiol 27:102–106

    CAS  Google Scholar 

  27. Zhang YJ, Wang JF, Huang YJ (2002) Screening for antitumor activities of endophytic fungi isolated from four species of gymnosperm. J Xiamen Univ (Nat Sci) 41:804–809

    Google Scholar 

Download references

Acknowledgments

This work was funded by the talent introduction plan of the Guizhou University (Grant No. [2014]59), the joint funds plan of the Guizhou province (Grant No. LH [2014]7657), and the grants of the agricultural science and technology foundation of Guizhou province (Grant Nos. NY[2013]3042 and NY[2013]3044) from the Science and Technology Bureau of Guizhou University and the Science and Technology Department of Guizhou province, China. The authors would also like to thank the National Natural Science Foundation of China (Grant No. 31460011) for supporting and funding this research.

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Authors and Affiliations

  1. Engineering and Research Center for Southwest Bio-Pharmaceutical Resources of National Education Ministry of China, Guizhou University, Guiyang, 550025, China

    Yi-Xin Qian, Ji-Chuan Kang, Jun-Jie Zhao & Jun He

  2. Department of Pharmacy, College of Pharmacy, Guizhou University, Guiyang, 550025, China

    Ji-Chuan Kang & Jun-Jie Zhao

  3. Department of Tobacco Sciences, College of Tobacco Sciences, Guizhou University, Guiyang, 550025, China

    Yi-Kai Luo

  4. Plant Protection and Quarantine Station of Guiyang, Guiyang, 550081, China

    Kun Geng

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  1. Yi-Xin Qian
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  2. Ji-Chuan Kang
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  3. Yi-Kai Luo
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  4. Jun-Jie Zhao
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  5. Jun He
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  6. Kun Geng
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Correspondence to Ji-Chuan Kang.

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Qian, YX., Kang, JC., Luo, YK. et al. A Bilobalide-Producing Endophytic Fungus, Pestalotiopsis uvicola from Medicinal Plant Ginkgo biloba . Curr Microbiol 73, 280–286 (2016). https://doi.org/10.1007/s00284-016-1060-6

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  • DOI: https://doi.org/10.1007/s00284-016-1060-6

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