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Taxonomic discrimination of flowering plants by multivariate analysis of Fourier transform infrared spectroscopy data

  • Physiology and Biochemistry
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Abstract

Fourier transform infrared spectroscopy (FTIR) provides biochemical profiles containing overlapping signals from a majority of the compounds that are present when whole cells are analyzed. Leaf samples of seven higher plant species and varieties were subjected to FTIR to determine whether plants can be discriminated phylogenetically on the basis of biochemical profiles. A hierarchical dendrogram based on principal component analysis (PCA) of FTIR data showed relationships between plants that were in agreement with known plant taxonomy. Genetic programming (GP) analysis determined the top three to five biomarkers from FTIR data that discriminated plants at each hierarchical level of the dendrogram. Most biomarkers determined by GP analysis at each hierarchical level were specific to the carbohydrate fingerprint region (1,200–800 cm−1) of the FTIR spectrum. Our results indicate that differences in cell-wall composition and structure can provide the basis for chemotaxonomy of flowering plants.

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Abbreviations

FTIR:

Fourier transform infrared spectroscopy

GP:

Genetic programming

PCA:

Principal component analysis

PyMS:

Pyrolysis mass spectrometry

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Acknowledgements

This work was supported by grants to JRL from the National Research Laboratory Program (M10104000234-01J000-10710), from the Strategic National R&D Program through the Genetic Resources and Information Network Center (no. BDM0100211), from the Korea Science and Engineering Foundation through the Plant Metabolism Research Center of the Kyung Hee University funded by the Korean Ministry of Science and Technology, and from the KRIBB Research Initiative Program.

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Authors and Affiliations

  1. Laboratories of Plant Genomic Services and Plant Cell Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 52 Eoun-dong, Yuseong-gu, Daejeon, 305-333, South Korea

    S. W. Kim, S. H. Ban, H. J. Chung & J. R. Liu

  2. Department of Chemistry, Hanyang University, 17 Haengdang-dong, Seongdong-gu, Seoul, 133-791, South Korea

    H. Chung & S. Cho

  3. Laboratory of Functional Genomics for Plant Secondary Metabolism (National Research Laboratory), Eugentech, Inc., 52 Eoun-dong, Yuseong-gu, Daejeon, 305-333, South Korea

    P. S. Choi

  4. Department of Biological Science, Korea Advanced Institute of Science and Technology, 373-1 Kusong-dong, Yuseong-gu, Daejeon, 305-701, South Korea

    O. J. Yoo

Authors
  1. S. W. Kim
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  2. S. H. Ban
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  3. H. Chung
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  4. S. Cho
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  5. H. J. Chung
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  6. P. S. Choi
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  7. O. J. Yoo
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  8. J. R. Liu
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Corresponding author

Correspondence to J. R. Liu.

Additional information

Communicated by J.M. Widholm

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Kim, S.W., Ban, S.H., Chung, H. et al. Taxonomic discrimination of flowering plants by multivariate analysis of Fourier transform infrared spectroscopy data. Plant Cell Rep 23, 246–250 (2004). https://doi.org/10.1007/s00299-004-0811-1

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  • DOI: https://doi.org/10.1007/s00299-004-0811-1

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