Abstract
We evaluated the phytotoxicity of mycotoxin ochratoxin A (OTA) from Aspergillus and Penicillium strains on Arabidopsis thaliana. The results demonstrate that the growth of Arabidopsis thaliana on media containing OTA was inhibited significantly. Moreover, OTA induced necrotic lesions in detached leaves, which are reminiscent of hypersensitive response lesions that are activated during plant–pathogen interactions and other abiotic stress factors. From our study, we can see that OTA exposure stimulated a biphasic oxidative burst in the leaves, resulting in the generation of hydrogen peroxide (H2O2) and superoxide anion radicals (O ·−2 ) and in the concomitant down-regulation of antioxidant enzyme defense responses and up-regulation of lipid peroxidation. These results suggested that OTA damage might result from reactive oxygen species pathways. Our experiments provide a useful model plant system for research on OTA-induced plant cell death.
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Abbreviations
- APX:
-
Ascorbate peroxidases
- CAT:
-
Catalases
- DAB:
-
3,3′-Diaminobenzidine
- DON:
-
Deoxynivalenol
- FB1:
-
Fumonisin B1
- H2DCFDA:
-
2′,7′-Dichlorodihydrofluorescein diacetate
- H2O2 :
-
Hydrogen peroxide
- HR:
-
Hypersensitive response
- MDA:
-
Malondialdehyde
- MES:
-
2-(N-morpholino) ethanesulphonic acid
- MS:
-
Murashige and Skoog
- NBT:
-
Nitroblue tetrazolium
- O ·−2 :
-
Superoxide anion radicals
- OTA:
-
Ochratoxin A
- PCD:
-
Programmed cell death
- POD:
-
Peroxidase
- PR:
-
Pathogenesis related
- ROS:
-
Reactive oxygen species
- SOD:
-
Superoxide dismutase
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This work was supported by the Ministry of Agriculture and the Ministry of Science and Technology of China.
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Communicated by K. Chong.
X.-L. Peng and W.-T. Xu contributed equally to this work.
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Peng, XL., Xu, WT., Wang, Y. et al. Mycotoxin Ochratoxin A-induced cell death and changes in oxidative metabolism of Arabidopsis thaliana . Plant Cell Rep 29, 153–161 (2010). https://doi.org/10.1007/s00299-009-0808-x
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DOI: https://doi.org/10.1007/s00299-009-0808-x