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Antioxidant enzyme activity of filamentous fungi isolated from Livingston Island, Maritime Antarctica

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Abstract

From 18 soil samples taken in the vicinity of the permanent Bulgarian Antarctic base “St. Kliment Ohridski” (62°38′29″S, 60°21′53″W) on Livingston Island, 109 filamentous fungi were isolated on selective media. The most widespread fungal species were members of the genera Cladosporium, Geomyces, Penicillium and Aspergillus. Other species, already recorded in Antarctic environment, were also isolated: Lecanicillium muscarium, Epicoccum nigrum and Alternaria alternata. Thirty strains demonstrating good growth were screened for antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) that play an important role in the defense of aerobic organisms against oxidative stress, by converting reactive oxygen species into nontoxic molecules. Six of them showed high enzyme activity. The tested strains produced SOD with statistically significant higher activity at 15°C than at 30°C suggesting that this enzyme is cold-active. Such SOD could be useful in medicine and cosmetics. The best producer of cold-active SOD, Aspergillus glaucus 363, cultivated in bioreactors, demonstrated optimal growth temperature at 25°C and maximum enzyme activities at 25 and 30°C for SOD and CAT, respectively. The electrophoretical analysis showed that the fungus possesses Cu/Zn-SOD.

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Acknowledgments

This work was supported by the National Scientific Fund of the Ministry of Education and Science, Bulgaria (grants VU-B-205/06, DO02-172/08 and BG051PO001-3.3.04/32), which is greatly acknowledged.

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Authors and Affiliations

  1. Sezione di Micologia, Dipartimento di Ecologia del Territorio, Università degli Studi di Pavia, Via S. Epifanio 14, 27100, Pavia, Italy

    S. Tosi

  2. The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Academician G. Bonchev 26, 1113, Sofia, Bulgaria

    N. Kostadinova, E. Krumova, S. Pashova, V. Dishliiska, B. Spassova, S. Vassilev & M. Angelova

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  1. S. Tosi
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  2. N. Kostadinova
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Correspondence to M. Angelova.

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Tosi, S., Kostadinova, N., Krumova, E. et al. Antioxidant enzyme activity of filamentous fungi isolated from Livingston Island, Maritime Antarctica. Polar Biol 33, 1227–1237 (2010). https://doi.org/10.1007/s00300-010-0812-1

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  • DOI: https://doi.org/10.1007/s00300-010-0812-1

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