Abstract
The reproductive mode of corals largely determines how zooxanthellae (Symbiodinium spp.) are acquired. Typically, broadcast spawning corals obtain symbionts from the surrounding environment, whereas most brooders transfer symbionts from maternal parent to offspring. Brooding corals are therefore predicted to harbor stable communities of Symbiodinium. This study documents the associations between Symbiodinium spp. and brooding corals in response to seasonal environmental fluctuations. Between March 2002 and December 2005, endosymbiont identity was determined seasonally from replicate colonies (n = 6) of three brooding species, Agaricia agaricites, Porites astreoides and Siderastrea radians, from shallow environments (1–4 m) of the Florida Keys and Bahamas. Symbionts were identified via denaturing gradient gel electrophoresis (DGGE) of the internal transcribed spacer 2 (ITS2) region. No change was detected in the Symbiodinium communities harbored within these brooding colonies. Additionally, no change in symbiosis was observed through a moderate bleaching event, thereby demonstrating that some bleached corals recover without changing symbionts.
References
Baker AC (2001) Reef corals bleach to survive change. Nature 411:765–766
Barneah O, Weis VM, Perez S, Benayahu Y (2004) Diversity of dinoflagellate symbionts in Red Sea corals: mode of symbiont acquisition matters. Mar Ecol Prog Ser 275:89–95
Berkelmans R, van Oppen MJH (2006) The role of zooxanthellae in the thermal tolerance of corals: a ‘nugget of hope’ for coral reefs in an era of climate change. Proc R Soc Lond B Biol Sci [doi:10.1098/rspb.2006.3567]
Buddemeier RW, Fautin DG (1993) Coral bleaching as an adaptive mechanism. BioScience 43:320–326
Douglas AE (1998) Host benefit and the evolution of specialization in symbiosis. Heredity 81:599–603
Goulet TL, Coffroth MA (2003) Stability of an octocoral–algal symbiosis over time and space. Mar Ecol Prog Ser 250:117–124
Hoegh-Guldburg O (1999) Climate change, coral bleaching, and the future of the world’s coral reefs. Mar Freshw Res 50:839–866
Iglesias-Prieto R, Beltran VH, LaJeunesse TC, Reyes-Bonilla H, Thome PE (2004) Different algal symbionts explain the vertical distribution of dominant reef corals in the eastern Pacific. Proc R Soc Lond B Biol Sci 271:1757–1763
LaJeunesse TC (2001) Investigating the biodiversity, ecology, and phylogeny of endosymbiontic dinoflagellates in the genus Symbiodinium using the ITS region: in search of a “species” level marker. J Phycol 37:866–880
LaJeunesse TC (2002) Diversity and community structure of symbiotic dinoflagellates from Caribbean coral reefs. Mar Biol 141:387–400
LaJeunesse TC (2005) “Species” radiations of symbiotic dinoflagellates in the Atlantic and Indo-Pacific since the Miocene-Pliocene transition. Mol Biol Evol 22:570–581
LaJeunesse TC, Bhagooli R, Hidaka M, deVantier L, Done T, Schmidt GW, Fitt WK, Hoegh-Guldberg O (2004) Differences in relative dominance beween closely related Symbiodinium spp. in coral reef host communities across environmental, latitudinal, and biogeographic gradients. Mar Ecol Prog Ser 284:147–161
Loh WK, Loi T, Carter D, Hoegh-Guldberg O (2001) Genetic variability of the symbiotic dinoflagellates from the wide ranging coral species Seriatopora hystrix and Acropora longicyathus in the Indo-West Pacific. Mar Ecol Prog Ser 222:97–107
van Oppen MJH (2004) Mode of zooxanthellae transmission does not affect zooxanthella diversity in acroporid corals. Mar Biol 144:1–7
van Oppen MJH, Palstra FP, Piquet AT, Miller DJ (2001) Patterns of coral-dinoflagellate associations in Acropora: significance of local availability and physiology of Symbiodinium strains and host-symbiont selectivity. Proc R Soc Lond Ser B 268:1759–1767
Richmond RH, Hunter CL (1990) Reproduction and recruitment of corals: comparisons among the Caribbean, the tropical Pacific and the Red Sea. Mar Ecol Prog Ser 60:185–203
Rowan R (1998) Diversity and ecology of zooxanthellae on coral reefs. J Phycol 34:407–417
Rowan R, Knowlton N (1995) Intraspecific diversity and ecological zonation in coral–algal symbiosis. Proc Natl Acad Sci USA 92:2850–2853
Rowan R, Powers DA (1991) A molecular genetic classification of zooxanthellae and the evolution of animal–algal symbiosis. Science 251:1348–1351
Rowan R, Knowlton N, Baker A, Jara J (1997) Landscape ecology of algal symbionts creates variation in episodes of coral bleaching. Nature 388:265–269
Santos SR, Taylor DJ, Kinzie RA III, Hidaka M, Sakai K, Coffroth MA (2002) Molecular phylogeny of symbiotic dinoflagellates inferred from partial chloroplast subunit (cp23S)-rDNA sequences. Mol Phyl Evol 23:97–111
Seutin G, White BN, Boag PT (1991) Preservation of avian blood and tissue samples for DNA analysis. Can J Zool 60:82–92
Thornhill DJ, LaJeunesse TC, Kemp DW, Fitt WK, Schmidt GW (2006) Multi-year, seasonal genotypic surveys of coral–algal symbioses reveal prevalent stability or post-bleaching reversion. Mar Biol 148:711–722
Toller WW, Rowan R, Knowlton N (2001) Repopulation of zooxanthellae in Caribbean corals Montastrea annularis and Montastrea faveolata following experimental and disease induced bleaching. Biol Bull 201:360–373
Trench RK (1993) Microalgal–invertebrate symbiosis: a review. Endocytobiosis and Cell Research 9:135–175
Acknowledgments
This research was funded by NSF (9906976 and 0137007) and the NOAA National Undersea Research Program through the Caribbean Marine Research Center on Lee Stocking Island in the Bahamas. An NSF Graduate Research Fellowship to D. J. Thornhill also supported this work. This project would not have been possible without the help of Dustin Kemp, Geoff Chilcoat, Tom Shannon, and Jennifer McCabe who assisted in sample collection. Jim Hendee, Louis Florit, NOAA and the CHAMP program graciously provided sea surface temperature data from Lee Stocking Island. We would also like to thank two anonymous reviewers for their helpful comments and suggestions.
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Thornhill, D.J., Fitt, W.K. & Schmidt, G.W. Highly stable symbioses among western Atlantic brooding corals. Coral Reefs 25, 515–519 (2006). https://doi.org/10.1007/s00338-006-0157-y
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DOI: https://doi.org/10.1007/s00338-006-0157-y