Abstract
Partially oxidized spherical silver nanoparticles (AgNPs) of different size are prepared by pulsed laser ablation in water and directly conjugated to protein S-ovalbumin for the first time and characterized by various optical techniques. UV–Visible spectrum of AgNPs showed localized surface plasmon resonance (LSPR) peak at 396 nm which red shift after protein addition. Further the increased concentration of AgNPs resulted a decrease in intensity and broadening of S-ovalbumin peak (278 nm), which can be related to the formation of protein NPs complex caused by the partial adsorption of S-ovalbumin on the surface of AgNPs. The red shift in LSPR peak of AgNPs after mixing with S-ovalbumin and decrease in protein-characteristic peak with increased silver loading confirmed the formation of protein–AgNPs bioconjugates. The effect of laser fluence on the size of AgNPs and nanoparticle–protein conjugation in the size range 5–38 nm is systematically studied. Raman spectra reveal broken disulphide bonds in the conjugated protein and formation of Ag–S bonds on the nanoparticle surface. Fluorescence spectroscopy showed quenching in fluorescence emission intensity of tryptophan residue of S-ovalbumin due to energy transfer from tryptophan moieties of albumin to AgNPs. Besides this, small blue shift in emission peak is also noticed in presence of AgNPs, which might be due to complex formation between protein and nanoparticles. The binding constant (K) and the number of binding sites (n) between AgNPs and S-ovalbumin have been found to be 0.006 M−1 and 7.11, respectively.
Similar content being viewed by others
References
J.P. Kottmann, O.J. Martin, D.R. Smith, S. Schultz, Phys. Rev. B 64, 235402 (2001)
G.T. Boyd, Th Rasing, J.R.R. Leite, Y.R. Shen, Phys. Rev. B 30, 519 (1984)
S. Schultz, D.R. Smith, J. J Mock, D. A. Schultz, PNAS 97, 996 (2000)
S.Y. Liau, D.C. Read, W.J. Pugh, J.R. Furr, A.D. Russell, Lett. Appl. Microbiol. 25, 279 (1997)
A. Gupta, S. Silver, Nat. Biotechnol. 16, 888 (1998)
K. Nomiya, A. Yoshizawa, K. Tsukagoshi, N.C. Kasuga, S. Hirakawa, J. Watanabe, J. Inorg. Biochem. 98, 46 (2004)
I. Sondiand, B. Salopek-Sondi, J. Colloid Interface Sci. 275, 177 (2004)
S. Petersen, J. Jakobi, S. Barcikowski, Appl. Surf. Sci. 255, 5435 (2009)
S. Petersen, S. Barcikowski, Adv. Funct. Mater. 19, 1167 (2009)
S. Besner, A.V. Kabashin, F.M. Winnik, M. Meunier, J. Phys. Chem. C 113, 9526 (2009)
J.P. Sylvestre, A.V. Kabashin, E. Sacher, M. Meunie, J.H.T. Luong, J. Am. Chem. Soc. 126, 7176 (2004)
J.P. Sylvestre, S. Poulin, A.V. Kabashin, E. Sacher, M. Meunier, J.H.T. Luong, J. Phys. Chem. B 108, 16864 (2004)
S. Petersen, S. BarcikowskiJ, Phys. Chem. C113, 19830 (2003)
J.M. Nam, C.S. Thaxtonand, C.A. Mirkin, Science 301, 1884 (2003)
A.G. Tkachenko, H. Xie, D. Coleman, W. Glomm, J. Ryan, M.F. Anderson, S. Franzen, D.L. Feldheim, J. Am. Chem. Soc. 125, 4700 (2003)
N. Wangoo, C.R. Suri, G. Shekhawat, Appl. Phys. Lett. 92, 133104 (2008)
I. Lynch, K.A. Dawson, Nanotoday 3, 40 (2008)
F. Mafune, J. Kohno, Y. Takeda, T. Kondow, H. Sawabe, J. Phys. Chem. B105, 5114 (2001)
T. Peters Jr, In All About Albumin: Biochemistry, Genetics, and Medical Applications (Academic Press, San Diego, 1996), p. 9
J.L. Burt, C.G. Wing, M.M. Yoshida, M.J. Yacama, Langmuir 20, 11778 (2004)
P.B. Kandagal, S. Ashoka, J. Seetharamappa, S.M.T. Shaikh, Y. Jadegoud, O.B. Ijare, J. Pharm. Biomed. Anal. 41, 393 (2006)
Y.Q. Wang, H.M. Zhang, G.C. Zhang, W.H. Tao, Z.H. Fei, Z.T. Liu, J. PharmBiomed. Anal. 43, 1869 (2007)
D. Zhang, O. Neumann, H. Wang, V.M. Yuwono, A. Bar-houmi, M. Perham, J.D. Hartgerink, P. Wittung-Stafshede, N.J. Halas, Nano Lett. 9, 666 (2009)
C.D. Keating, K.M. Kovaleski, M.J. Natan, J. Phys. Chem. B102, 9404 (1998)
S. Chah, M.R. Hammond, R.N. Zare, Chem. Biol. 12, 323 (2005)
N. Biswas, A.J. Waring, F.J. Walther, R.A. Dluhy, Biochim. Biophys. Acta 1768, 1070 (2007)
R.J. Clark, R.E. Hester, Spectroscopy of Biological Systems (John Wiley & Sons Ltd, New York, 1986)
T. Miura, H. Takeuchi, I. Harada, Biochemistry 27, 88 (1988)
C.S. Levin, B. GJanesko, R. Bardhan, G.E. Scuseria, J. DHartgerink, N.J. Halas, Nano Lett. 6, 2617 (2006)
R.F. Chenhas, Arch. Biochem. Biophys. 142, 552 (1971)
K.H. Ulrich, Pharmacol. Rev. 33, 17 (1981)
B.F. Pan, F. Gao, L.M. Ao, J. Magnes. Magn. Mater. 293, 252 (2005)
R.F. Chenhas, Arch. Biochem. Biophys. 168, 605 (1973)
J.R. Lakowicz, Principles of Fluorescence Spectroscopy, 3rd edn. (Springer, New York, 2006)
A.C. Tedesco, D.M. Oliveira, Z.G.M. Lacava, R.B. Azevedo, E.C.D. Lima, P.C. Morais, J. Magnes. Magn. Mater. 272–276, 2404 (2004)
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Joshi, D., Soni, R.K. Laser-induced synthesis of silver nanoparticles and their conjugation with protein. Appl. Phys. A 116, 635–641 (2014). https://doi.org/10.1007/s00339-013-8210-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00339-013-8210-5