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Dose-Dependent Effects of Coronatine on Cotton Seedling Growth Under Salt Stress

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Abstract

Coronatine (COR) is a chlorosis-inducing, non-host-specific phytotoxin produced by several members of the Pseudomonas syringae group of pathovars. It acts as a molecular mimic of methyl jasmonate. This study investigated the dose-dependent effects of COR on cotton seedling (Gossypium hirsutum L. cv. CCRI 45) growth under normal or salt-stressed conditions. The results demonstrated that salt stress inhibited growth of cotton seedlings, decreased chlorophyll content and net photosynthesis, altered activity of antioxidant enzymes (Superoxide dismutase, Catalase, and peroxidases), and ion balance within the stressed seedlings. Pretreatment with a low concentration of COR (0.01 μmol/L) increased seedling biomass under unstressed and salt-stressed conditions by increasing chlorophyll contents, net photosynthesis and PSII function, elevating the activity of antioxidative enzymes and protein content, improving Na+ exclusion, K+ retention, and the K+/Na+ ratio, and reducing lipid peroxidation causing membrane damage. However, high concentrations of COR (1 μmol/L) decreased seedling biomass under unstressed conditions, and exacerbated the above-mentioned adverse effects of salt stress by inhibiting the activity of antioxidative enzymes and photosynthesis, enhancing chlorophyll degradation and lipid peroxidation causing membrane damage. These results indicated that COR has dose-dependent effects on cotton growth. A low concentration (0.01 μmol/L) of COR improved seedling growth and alleviated the inhibition due to salt stress. However, a higher concentration (1 μmol/L) of COR inhibited seedling growth and aggravated salt stress in cotton seedling under salt-stressed conditions.

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Acknowledgments

The authors thank Dr. Carol L. Bender, Professor of Oklahoma State University, for kindly providing standard COR samples. Special acknowledgements are given to the editors and reviewers. This research was supported by the National Natural Science Foundation of China (No. 31101096), and National Key Technologies R&D Program of China (No. 2013BAD05B02).

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Authors and Affiliations

  1. Key Laboratory of Agricultural Water Resources, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Huaizhong RD 286, Shijiazhuang, 050021, People’s Republic of China

    ZhiXia Xie & Xiaojing Liu

  2. State Key Laboratory of Plant Physiology and Biochemistry, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100094, People’s Republic of China

    LiuSheng Duan & ZhaoHu Li

  3. Beijing Research Center of Intelligent Equipment for Agriculture, Beijing, 100097, People’s Republic of China

    XiaoDong Wang

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  1. ZhiXia Xie
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Xie, Z., Duan, L., Li, Z. et al. Dose-Dependent Effects of Coronatine on Cotton Seedling Growth Under Salt Stress. J Plant Growth Regul 34, 651–664 (2015). https://doi.org/10.1007/s00344-015-9501-1

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