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Diversity of heterotrophic aerobic cultivable microbial communities of soils treated with fumigants and dynamics of metabolic, microbial, and mineralization quotients

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Abstract

A combination of molecular and classical techniques was used to study the composition, structure, diversity, and dynamics of an aerobic heterotrophic cultivable bacterial community isolated from five different soil samples treated with the fumigant agent 1,3-dichloropropene (1,3-D) and further subjected to nitrogen–phosphorous–potassium (NPK) fertigation (F), amendment (C 2 and C 4), and NPK fertigation plus amendment (F + C) in two different periods (May and July). The restriction and sequence analysis of 16S rDNA from 189 isolates revealed a very high percentage (94%) of Gram-positive bacterial isolates, most of which (83%) belonging to the genus Bacillus. The degree of intraspecific genetic diversity was high, as shown by random amplified polymorphic DNA analysis. These data seem to be related with the increase in microbial biomass C (C mic) content and the decrease in the total organic C (C org) and metabolic quotient (qCO2) values, especially in amended soils (C 2, C 4) where soil microflora mineralized the organic matter of the added fertilizers. In a short term, it is suggested that the presence of very high percentage of Gram-positive bacteria might be related to the ability of these bacteria to form spores so as to be resistant to fumigants rather than being the result of a selective pressure in the predominance of microbial species with a set of genes involved in biodegradation of 1,3-D.

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Acknowledgment

We are very grateful to two anonymous reviewers and to the Editor-in-chief for the revision of the manuscript and for their useful suggestions and comments in improving the manuscript.

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Authors and Affiliations

  1. C.R.A.-Istituto Sperimentale per la Nutrizione delle Piante, Via della Navicella 2-4, 00184, Rome, Italy

    Stefano Mocali & Anna Benedetti

  2. Dipartimento di Scienze e Tecnologie Ambientali Forestali, University of Florence, Via S. Bonaventura, 13, 50145, Florence, Italy

    Donatella Paffetti & Giovanni Emiliani

  3. Dipartimento di Biologia Animale e Genetica, University of Florence, Via Romana 17-19, 50125, Florence, Italy

    Renato Fani

Authors
  1. Stefano Mocali
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  2. Donatella Paffetti
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  3. Giovanni Emiliani
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  4. Anna Benedetti
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  5. Renato Fani
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Correspondence to Renato Fani.

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Stefano Mocali and Donatella Paffetti contributed equally to this work.

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Mocali, S., Paffetti, D., Emiliani, G. et al. Diversity of heterotrophic aerobic cultivable microbial communities of soils treated with fumigants and dynamics of metabolic, microbial, and mineralization quotients. Biol Fertil Soils 44, 557–569 (2008). https://doi.org/10.1007/s00374-007-0235-5

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  • DOI: https://doi.org/10.1007/s00374-007-0235-5

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