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Effects of intercropping and Rhizobium inoculation on yield and rhizosphere bacterial community of faba bean (Vicia faba L.)

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Abstract

The effects of intercropping with maize and Rhizobium inoculation on the yield of faba bean and rhizosphere bacterial diversity were analyzed by terminal restriction fragment length polymorphism, amplified 16S rDNA restriction analysis (ARDRA), and 16S rDNA sequencing. The results showed that intercropping but not Rhizobium inoculation significantly increased the faba bean yield. Probably the relatively high level of native rhizobia in soil annulled the effect of rhizobia inoculation. ARDRA results showed that intercropping did not affect bacterial diversity whereas Rhizobium inoculation decreased bacterial diversity. The canonical correspondence analysis showed that the composition of bacterial community was changed apparently by intercropping, and there was a positive correlation (P = 0.724) between faba bean yields and intercropping and an apparent correlation (P = 0.648) between intercropping and total N. The available content of K and P had a lower effect on the bacterial community composition than did the total N content, Rhizobium inoculation, and microbial biomass C. Rhizobium inoculation negatively correlated with microbial biomass C (P = −0.827). These results revealed a complex interaction among the intercropped crops, inoculation with rhizobia, and indigenous bacteria and implied that the increase of faba bean production in intercropping might be related to the modification of rhizosphere bacterial community.

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Acknowledgments

This study was funded by the National Basic Research (973) program of China (project no. 2006CB100206). We thank Mr. Jian Hao Sun and Professor Xing Guo Bao for technical assistance. The field experiments were performed with the assistance from the Institute of Soil Science and Fertilizers, Gansu Academy of Agricultural Sciences. Ms. Hui Min Gao helped us in the sample collection. We thank Professor Kate Scow for her valuable comments on the manuscript.

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  1. State Key Lab for Agrobiotechnology, Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture, Center of Biomass Engineering, College of Biological Sciences, China Agricultural University, Beijing, 100193, China

    Nan Nan Zhang, Yan Mei Sun, Wen Xin Chen & Hong Li Yuan

  2. College of Resources and Environmental Sciences, China Agricultural University, Beijing, 100193, China

    Long Li

  3. Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, 11340, Federal District, Mexico

    En Tao Wang

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  1. Nan Nan Zhang
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Correspondence to Hong Li Yuan.

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Zhang, N.N., Sun, Y.M., Li, L. et al. Effects of intercropping and Rhizobium inoculation on yield and rhizosphere bacterial community of faba bean (Vicia faba L.). Biol Fertil Soils 46, 625–639 (2010). https://doi.org/10.1007/s00374-010-0469-5

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