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Fungal degradation of chlorpyrifos by Acremonium sp. strain (GFRC-1) isolated from a laboratory-enriched red agricultural soil

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Abstract

The organophosphorus insecticide, chlorpyrifos, has been widely applied in agriculture; in veterinary, against household pests; and in subterranean termite control. Due to its slow rate of degradation in soil, it can persist for extended periods in soil with a significant threat to environment and public health. The mixed and pure fungi were isolated from three soils by enrichment technique. The enriched mixed fungal cultures were capable of biodegrading chlorpyrifos (300 mg L−1) when cultivated in Czapek Dox medium. The identified pure fungal strain, Acremonium sp., utilized chlorpyrifos as a source of carbon and nitrogen. The highest chlorpyrifos degradation (83.9%) by Acremonium sp. strain GFRC-1 was found when cultivated in the nutrient medium with full nutrients. Desdiethyl chlorpyrifos was detected as a major biodegradation product of chlorpyrifos. The isolated fungal strain will be used for developing bioremediation strategy for chlorpyrifos-polluted soils.

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Acknowledgment

The authors thank Indian Council of Agricultural Research for providing financial assistance to the Emeritus Scientists scheme to carry out this research work. Thanks are also due to Dr S. Walia for the help in LC-MS-MS analysis of the sample. The technical assistance of Mr. B. Tripathi is appreciated.

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  1. Division of Agricultural Chemicals, Indian Agricultural Research Institute, New Delhi, 110012, India

    Gita Kulshrestha & Anupriya Kumari

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  1. Gita Kulshrestha
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  2. Anupriya Kumari
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Correspondence to Gita Kulshrestha.

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Kulshrestha, G., Kumari, A. Fungal degradation of chlorpyrifos by Acremonium sp. strain (GFRC-1) isolated from a laboratory-enriched red agricultural soil. Biol Fertil Soils 47, 219–225 (2011). https://doi.org/10.1007/s00374-010-0505-5

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  • DOI: https://doi.org/10.1007/s00374-010-0505-5

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