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Maize lines with different nitrogen use efficiency select bacterial communities with different β-glucosidase-encoding genes and glucosidase activity in the rhizosphere

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Abstract

We studied the molecular diversity of β-glucosidase-encoding genes, microbial biomass, cellulase, N-acetyl-glucosaminidase, β-glucosidase, and β-galactosidase activities in the rhizosphere and bulk soil of two maize lines differing in nitrogen use efficiency (NUE). The maize lines had significant differences in diversity of β-glucosidase-encoding genes in their rhizosphere, and Actinobacteria and Proteobacteria were the dominating phyla in all samples, but representatives of Bacteroidetes, Chloroflexi, Deinococcus-Thermus, Firmicutes, and Cyanobacteria were also detected. Among the Proteobacteria, β-glucosidase genes from α-, β-, and γ-Proteobacteria were dominant in the rhizosphere of the high NUE maize line, whereas δ-Proteobacteria β-glucosidase genes were dominant in the rhizosphere of the low NUE maize line. The high NUE maize line also showed higher glucosidase activities in the rhizosphere than the low NUE maize line. We concluded that plants with high NUE select bacterial communities in the rhizosphere differing in the diversity of β-glucosidase-encoding genes which likely result in higher C-hydrolyzing enzyme activities. These effects on the diversity of β-glucosidase-encoding genes may influence the C dynamics in the agro-ecosystems.

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Acknowledgments

This research was supported by the Ministry for Education and Research project “PRIN 2009MWY5F9.” Shamina I. Pathan was supported by the Marie Curie ITN action “TRAINBIODIVERSE,” grant no. 289949. The Department of Agrifood Production and Environmental Sciences thanks the Ente Cassa di Risparmio di Firenze for the financial support for the acquisition of new instruments.

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Authors and Affiliations

  1. Department of Agrifood Production and Environmental Sciences, University of Florence, Piazzale delle Cascine 28, 50144, Florence, Italy

    Shamina I. Pathan, Maria Teresa Ceccherini, Laura Giagnoni, Judith Ascher, Mariarita Arenella, Giacomo Pietramellara, Paolo Nannipieri & Giancarlo Renella

  2. Section of Microbiology, Institute of Biology, Faculty of Science, University of Copenhagen, Copenhagen, Denmark

    Martin Asser Hansen & Søren J. Sørensen

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  1. Shamina I. Pathan
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  2. Maria Teresa Ceccherini
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Correspondence to Giancarlo Renella.

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Pathan, S.I., Ceccherini, M.T., Hansen, M.A. et al. Maize lines with different nitrogen use efficiency select bacterial communities with different β-glucosidase-encoding genes and glucosidase activity in the rhizosphere. Biol Fertil Soils 51, 995–1004 (2015). https://doi.org/10.1007/s00374-015-1045-9

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