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Colloid and Polymer Science
Erschienen in: Colloid and Polymer Science 8/2015

01.08.2015 | Original Contribution

Reactive poly(ethylene-co-vinyl alcohol) monoliths with tunable pore morphology for enzyme immobilization

verfasst von: Guowei Wang, Hiroshi Uyama

Erschienen in: Colloid and Polymer Science | Ausgabe 8/2015

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Abstract

Poly(ethylene-co-vinyl alcohol) (EVOH) monoliths were fabricated from two kinds of EVOH with different ethylene contents by thermally induced phase separation (TIPS) technique using a mixed solvent of isopropanol (IPA) and water. The pore and skeleton sizes could be controlled by changing the fabrication parameters such as the concentration of the polymer, the ratio of IPA and water, and the cooling temperature. The EVOH monoliths possessed relatively high specific surface area and uniform mesopore structure. Furthermore, the monolith was applied for a support matrix of enzyme immobilization. The monolith was successfully activated by carbonyldiimidazole and subsequently reacted with urease. The good reusability of the urease-immobilized monolith was demonstrated. The present EVOH monoliths have good hydrophilicity and reactive hydroxyl groups; therefore, it possesses large potential for various bio-related applications such as enzyme immobilization and protein purification.
Vorheriger Artikel Effect of alkali-soluble resin emulsifiers on coalescence and interdiffusion between latex polymer particles
Nächster Artikel Enhanced permeability of Rhodamine B into bilayers comprised of amphiphilic random block copolymers by incorporation of ionic segments in the hydrophobic chains

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Metadaten
Titel
Reactive poly(ethylene-co-vinyl alcohol) monoliths with tunable pore morphology for enzyme immobilization
verfasst von
Guowei Wang
Hiroshi Uyama
Publikationsdatum
01.08.2015
Verlag
Springer Berlin Heidelberg
Erschienen in
Colloid and Polymer Science / Ausgabe 8/2015
Print ISSN: 0303-402X
Elektronische ISSN: 1435-1536
DOI
https://doi.org/10.1007/s00396-015-3637-1

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