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Preparation of gentamicin-loaded electrospun coating on titanium implants and a study of their properties in vitro

  • Basic Science
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Archives of Orthopaedic and Trauma Surgery Aims and scope Submit manuscript

Abstract

Purpose

Implant-related infections are disastrous complications in the clinic, and there are no effective therapies. In this preliminary study, gentamicin-loaded coating on titanium implants was prepared using the electrospinning technique, and some properties of the coating titanium implants were studied.

Methods

We adopted the electrospinning technique to prepare gentamicin-coated titanium implants. The surface structure of the coating implants was observed using scanning electron microscope. An elution study was performed to determine the release behavior of the gentamicin from the coating. The antibacterial efficacy and quantitative analysis of the bacterial adhesion of Staphylococcus aureus were evaluated in vitro, and the cytotoxicity of the coated titanium implants on osteoblasts was investigated in vitro.

Results

The morphology of the gentamicin-coated titanium implants exhibited nanofibers, and the release of gentamicin showed an initial gentamicin burst followed by a slow release. The gentamicin-coated titanium implants had a persistent antibacterial efficacy for 1 week and significantly reduced the adhesion of the Staphylococcus aureus compared with bare titanium implants in vitro. There was no cytotoxicity observed in vitro for the gentamicin-coated implants.

Conclusion

The gentamicin-coated titanium implants, which were prepared using an electrospinning technique, present many advantages and may be considered to prevent and treat the implant-related infections.

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Acknowledgments

We gratefully acknowledge the contributions of State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China and we thank Health Medical Treatment Apparatus Co., Ltd. Suzhou, China for supplying the three-hole titanium implants.

Conflict of interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. Department of Orthopaedics, Nanjing First Hospital, Nanjing Medical University, Nanjing, 210006, China

    Liang-liang Li, Li-ming Wang & Yan Xu

  2. State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China

    Lan-xin Lv

Authors
  1. Liang-liang Li
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  2. Li-ming Wang
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  3. Yan Xu
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  4. Lan-xin Lv
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Correspondence to Li-ming Wang.

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Li, Ll., Wang, Lm., Xu, Y. et al. Preparation of gentamicin-loaded electrospun coating on titanium implants and a study of their properties in vitro. Arch Orthop Trauma Surg 132, 897–903 (2012). https://doi.org/10.1007/s00402-012-1490-y

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  • DOI: https://doi.org/10.1007/s00402-012-1490-y

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