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Involvement of root ABA and hydraulic conductivity in the control of water relations in wheat plants exposed to increased evaporative demand

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Abstract

We studied the possible involvement of ABA in the control of water relations under conditions of increased evaporative demand. Warming the air by 3°C increased stomatal conductance and raised transpiration rates of hydroponically grown Triticum durum plants while bringing about a temporary loss of relative water content (RWC) and immediate cessation of leaf extension. However, both RWC and extension growth recovered within 30 min although transpiration remained high. The restoration of leaf hydration and growth were enabled by increased root hydraulic conductivity after increasing the air temperature. The use of mercuric chloride (an inhibitor of water channels) to interfere with the rise on root hydraulic conductivity hindered the restoration of extension growth. Air warming increased ABA content in roots and decreased it in shoots. We propose this redistribution of ABA in favour of the roots which increased the root hydraulic conductivity sufficiently to permit rapid recovery of shoot hydration and leaf elongation rates without the involvement of stomatal closure. This proposal is based on known ability of ABA to increase hydraulic conductivity confirmed in these experiments by measuring the effect of exogenous ABA on osmotically driven flow of xylem sap from the roots. Accumulation of root ABA was mainly the outcome of increased export from the shoots. When phloem transport in air-warmed plants was inhibited by cooling the shoot base this prevented ABA enrichment of the roots and favoured an accumulation of ABA in the shoot. As a consequence, stomata closed.

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Abbreviations

RWC:

Relative water content

ABA:

Abscisic acid

Ψl :

Leaf water potential

R:

Hydraulic resistance

E:

Evaporation rate

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Acknowledgments

This work was supported by grant of the Russian Fond of Basic Researches (RFBR No. 08-04-97021, RFBR 10-04-97020).

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Authors and Affiliations

  1. Ufa Research Centre, Russian Academy of Sciences, 450054, Ufa, Russia

    Guzel Kudoyarova, Svetlana Veselova, Rashit Farhutdinov, Dmitry Veselov & Guzyal Sharipova

  2. Julius von Sachs Institut für Biowissenschaften der Universität, Lehrstuhl Botanik I, Julius von Sachs Platz 2, 97082, Würzburg, Germany

    Wolfram Hartung

Authors
  1. Guzel Kudoyarova
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  2. Svetlana Veselova
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  3. Wolfram Hartung
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  4. Rashit Farhutdinov
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  5. Dmitry Veselov
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  6. Guzyal Sharipova
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Correspondence to Guzel Kudoyarova.

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Kudoyarova, G., Veselova, S., Hartung, W. et al. Involvement of root ABA and hydraulic conductivity in the control of water relations in wheat plants exposed to increased evaporative demand. Planta 233, 87–94 (2011). https://doi.org/10.1007/s00425-010-1286-7

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  • DOI: https://doi.org/10.1007/s00425-010-1286-7

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