Abstract
Laboratory bioassays on insecticidal activity of essential oils (EOs) extracted from six Mediterranean plants (Achillea millefolium, Lavandula angustifolia, Helichrysum italicum, Foeniculum vulgare, Myrtus communis, and Rosmarinus officinalis) were carried out against the larvae of the Culicidae mosquito Aedes albopictus. The chemical composition of the six EOs was also investigated. Results from applications showed that all tested oils had insecticidal activity, with differences in mortality rates as a function of both oil and dosage. At the highest dosage (300 ppm), EOs from H. italicum, A. millefolium, and F. vulgare caused higher mortality than the other three oils, with mortality rates ranging from 98.3% to 100%. M. communis EO induced only 36.7% larval mortality at the highest dosage (300 ppm), a similar value to those recorded at the same dosage by using R. officinalis and L. angustifolia (51.7% and 55%, respectively). Identified compounds ranged from 91% to 99%. The analyzed EOs had higher content of monoterpenoids (80–99%) than sesquiterpenes (1–15%), and they can be categorized into three groups on the basis of their composition. Few EOs showed the hydrocarbon sesquiterpenes, and these volatile compounds were generally predominant in comparison with the oxygenated forms, which were detected in lower quantities only in H. italicum (1.80%) and in M. communis (1%).
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References
Abbott WS (1925) A method of computing the effectiveness of an insecticide. J Econ Entomol 18:265–267
Amer A, Mehlhorn H (2006a) Persistency of larvicidal effect of plant oil extracted under different storage conditions. Parasitol Res 99:473–477
Amer A, Mehlhorn H (2006b) Larvicidal effect of various essential oils against Aedes, Anopheles and Culex larvae (Diptera, Culicidae). Parasitol Res 99:466–472
Anees AM (2008) Larvicidal activity of Ocimum sanctum Linn. (Labiatae) against Aedes aegypti (L.) and Culex quinquefasciatus (Say). Parasitol Res 103(6):1451–1453
Araujo ECC, Silveira ER, Lima MAS, Andrade Neto M, De Andrade IL, Lima MAA, Santiago GMP, Mesquita ALM (2003) Insecticidal activity and chemical composition of volatile oils from Hyptis martiusii Benth. J Agric Food Chem 51(13):3760–3762
Carvalho AFU, Maciel Melo VM, Craveiro AA, Machado MIL, Bantim MB, Rabelo E (2003) Larvicidal activity of the essential oil from Lippia sidoides Cham. against Aedes aegypti L. Mem Inst Oswaldo Cruz Rio de Janiero 98(4):569–571
Chantraine JM, Laurent D, Ballivian C, Saavedra G, Ibanez R, Vilaseca LA (1998) Insecticidal activity of essential oils on Aedes aegypti larvae. Phytother Res 12(5):350–354
Cheng S, Liu J, Tsai K, Chen W, Chang S (2004) Chemical composition and mosquito larvicidal activity of essential oils from leaves of different Cinnamomum osmophloeum provenances. J Agric Food Chem 52:4395–4400
Cheng SS, Chang HT, Chang ST, Tsai KH, Chen WJ (2003) Bioactivity of selected plant essential oils against the yellow fever mosquito Aedes aegypti larvae. Bioresour Technol 89:99–102
Choi W, Park B, Ku S, Lee S (2002) Repellent activity of essential oils and monoterpenes against Culex pipiens pallens. J Am Mosq Control Assoc 18(4):348–351
Evergetis E, Michaelakis A, Kioulos E, Koliopoulos G, Haroutounian SA (2009) Chemical composition and larvicidal activity of essential oils from six Apiaceae family taxa against the West Nile virus vector Culex pipiens. Parasitol Res 105(1):117–124
Italian Pharmacopoeia (2008) Farmacopea Ufficiale Italiana, XII edizione, Roma.
Jaenson TGT, Palsson K, Borg-Karlson A (2006) Evaluation of extracts and oils of mosquito repellent plants from Sweden and Guinea-Bissau. J Med Entomol 43(1):113–119
James AA (1992) Mosquito molecular genetics: the hands that feed bite back. Science 257(5066):37–38
Kamaraj C, Bagavan A, Rahuman AA, Zahir AA, Elango G, Pandiyan G (2009) Larvicidal potential of medicinal plant extracts against Anopheles subpictus Grassi and Culex tritaeniorhynchus Giles (Diptera: Culicidae). Parasitol Res 104(5):1163–1171
Kannathasan K, Senthilkumar A, Venkatesalu V, Chandrasekaran M (2008) Larvicidal activity of fatty acid methyl esters of Vitex species against Culex quinquefasciatus. Parasitol Res 103(4):999–1001
Koliopoulos G, Pitarokili D, Kioulos E, Michaelakis A, Tzakou O (2010) Chemical composition and larvicidal evaluation of Mentha, Salvia, and Melissa essential oils against the West Nile virus mosquito Culex pipiens. Parasitol Res 107(2):327–335
Lee C, Sung B, Lee H (2006) Acaricidal activity of fennel seed oils and their main components against Tyrophagus putrescentiae, a stored-food mite. J Stored Prod Res 42:8–14
Mathew N, Anitha MG, Bala TSL, Sivakumar SM, Narmadha R, Kalyanasundaram M (2009) Larvicidal activity of Saraca indica, Nyctanthes arbor-tristis and Clitoria ternatea extracts against three mosquito vector species. Parasitol Res 104(5):1017–1025
Michaelakis A, Papachristos D, Kimbaris A, Koliopoulos G, Giatropoulos A, Polissiou MG (2009) Citrus essential oils and four enantiomeric pinenes against Culex pipiens (Diptera: Culicidae). Parasitol Res 105(3):769–773
Mimica-Dukić N, Kujundžić S, Soković M, Couladis M (2003) Essential oils composition and antifungal activity of Foeniculum vulgare Mill. obtained by different distillation conditions. Phytother Res 17(4):368–371
Moretti MD, Sanna-Passino G, Demontis S, Bazzoni E (2002) Essential oil formulation useful as a new tool for insect pest control. AAPs PharmSciTech, 3 (2) article 13, http://www.aapspharmscitech.org.
Pavela R, Vrchotová N, Tříska J (2009) Mosquitocidal activities of thyme oils (Thymus vulgaris L.) against Culex quinquefasciatus (Diptera: Culicidae). Parasitol Res 105(5):1365–1370
Peng Z, Yang J, Wang H, Simons FER (1999) Production and characterisation of monoclonal antibodies to two new mosquito Aedes aegypti salivary protein. Insect Biochem Mol Biol 29(10):909–914
Prajapati V, Tripathi AK, Aggrawal KK, Khanuja SPS (2005) Insecticidal, repellent and oviposition-deterrent activity of selected essential oils against Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus. Bioresour Technol 96:1749–1757
Rahuman AA, Venkatesan P, Gopalakrishnan G (2008) Mosquito larvicidal activity of oleic and linoleic acids isolated from Citrullus colocynthis (Linn.) Schrad. Parasitol Res 103(6):1383–1390
Rahuman AA, Gopalakrishnan G, Ghouse BS, Arumugam S, Himalayan B (2000) Effect of Feronia limonia on mosquito larvae. Fitoterapia 71:553–555
Saad E, Hussien R, Saher F, Ahmed Z (2006) Acaricidal activity of some essential oils and their monoterpenoidal constituents against house dust mite, Dermatophagoides pteronyssinus (Acari: Pyroglyphidae). J Zhejiang Univ 7(12):957–962
Senthilkumar A, Kannathasan K, Venkatesalu V (2008) Chemical constituents and larvicidal property of the essential oil of Blumea mollis (D. Don) Merr. against Culex quinquefasciatus. Parasitol Res 103(3):959–962
Severini C, Romi R, Marinucci M, Rajmond M (1993) Mechanism of insecticide resistance in field populations of Culex pipiens from Italy. J Am Mosq Control Assoc 9:164–168
Sokal RR, Rohlf FJ (1981) Biometry. Freeman and Company, New York
Traboulsi AF, El-Haj S, Tueni M, Taoubi K, Abi Nader N, Mrad A (2005) Repellency and toxicity of aromatic plant extracts against the mosquito Culex pipiens molestus (Diptera: Culicidae). Pest Manag Sci 61(6):597–604
Traboulsi AF, Taoubi K, El-Haj S, Bessiere JM, Rammal S (2002) Insecticidal properties of essential plant oils against the mosquito Culex pipiens molestus (Diptera: Culicidae). Pest Manag Sci 58(5):491–495
Tunç I, Berger BM, Erler F, Dağc F (2000) Ovicidal activity of essential oils from five plants against two stored-product insects. J Stored Prod Res 36:161–168
Tunòn H, Horsell W, Bohlin L (1994) Mosquito repellent activity of compounds occurring in Achillea millefolium L. (Asteraceae). Econ Bot 48(2):111–120
World Health Organization (1981) Instruction for determining the susceptibility or resistance of mosquito larvae to insecticide. WHO/VBC/81.807
Yang YC, Lee SG, Lee HK, Kim MK, Lee SH, Lee HS (2002) A piperidine amide extracted from Piper longum L. fruit shows activity against Aedes aegypti mosquito larvae. J Agric Food Chem 50:3765–3767
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Conti, B., Canale, A., Bertoli, A. et al. Essential oil composition and larvicidal activity of six Mediterranean aromatic plants against the mosquito Aedes albopictus (Diptera: Culicidae). Parasitol Res 107, 1455–1461 (2010). https://doi.org/10.1007/s00436-010-2018-4
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DOI: https://doi.org/10.1007/s00436-010-2018-4