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Response surface method to optimize the production and characterization of lipase from Penicillium verrucosum in solid-state fermentation

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Abstract

Current studies about lipase production by solid-state fermentation involve the use of agro-industrial residues towards developing cost-effective systems directed to large-scale commercialization of enzyme-catalyzed processes. In this work, lipase production and partial characterization of the crude enzymatic extracts obtained by Penicillium verrucosum using soybean bran as substrate was investigated. Different inductors were evaluated and the results showed that there is no influence of this variable on the lipase production, while temperature and initial moisture were the main factors that affected enzyme production. The optimized cultivation temperature (27.5 °C) and initial moisture of substrate (55%) were determined using the response surface methodology. Kinetics of lipase production was followed at the optimized growth conditions. Optimum lipase yield was 40 U/g of dry bran. The crude enzymatic extract showed optimal activity in the range from 30 to 45 °C and in pH 7.0.

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Acknowledgments

The authors thank CAPES and CNPq for the financial support of this work and scholarships.

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Authors and Affiliations

  1. Programa de Mestrado em Engenharia de Alimentos, Universidade Regional Integrada do Alto Uruguai e das Missões - Campus de Erechim, Av. Sete de Setembro, 1621, 99700-000, Erechim, RS, Brazil

    Aniela Pinto Kempka, Nadia Lamb Lipke, Thais da Luz Fontoura Pinheiro, Silvana Menoncin, Helen Treichel, Marco Di Luccio & Débora de Oliveira

  2. Depto. de Bioquímica - IQ/UFRJ, Centro de Tecnologia, Bl. A, Sala 549-2, Rio de Janeiro, 21945-900, RJ, Brazil

    Denise M. G. Freire

Authors
  1. Aniela Pinto Kempka
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  2. Nadia Lamb Lipke
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  3. Thais da Luz Fontoura Pinheiro
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  4. Silvana Menoncin
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  5. Helen Treichel
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  6. Denise M. G. Freire
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  7. Marco Di Luccio
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  8. Débora de Oliveira
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Correspondence to Débora de Oliveira.

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Kempka, A.P., Lipke, N.L., da Luz Fontoura Pinheiro, T. et al. Response surface method to optimize the production and characterization of lipase from Penicillium verrucosum in solid-state fermentation. Bioprocess Biosyst Eng 31, 119–125 (2008). https://doi.org/10.1007/s00449-007-0154-8

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  • DOI: https://doi.org/10.1007/s00449-007-0154-8

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