Abstract
This study describes the production of xylanases from Aspergillus niveus, A. niger, and A. ochraceus under solid-state fermentation using agro-industrial residues as substrates. Enzyme production was improved using a mixture of wheat bran and yeast extract or peptone. When a mixture of corncob and wheat bran was used, xylanase production from A. niger and A. ochraceus increased by 18%. All cultures were incubated at 30 °C at 70–80% relative humidity for 96 h. For biobleaching assays, 10 or 35 U of xylanase/g dry cellulose pulp were incubated at pH 5.5 for 1 or 2 h, at 55 °C. The delignification efficiency was 20%, the brightness (percentage of ISO) increased two to three points and the viscosity was maintained confirming the absence of cellulolytic activity. These results indicated that the use of xylanases could help to reduce the amount of chlorine compounds used in cellulose pulp treatment.
Similar content being viewed by others
References
Angayarknni J, Palaniswamy M, Pradeep BV, Swaminathan K (2006) Biochemical substitution of fungal xylanases for prebleaching of hardwood kraft pulp. Afr J Biotechnol 5:921–929
Polizeli MLTM, Rizzatti ACS, Monti R, Terenzi HF, Jorge JA, Amorim DS (2005) Xylanases from fungi: properties and industrial applications. Appl Microbiol Biotechnol 67:577–591
Bajpai P (1999) Application of enzymes in the pulp and paper industry. Biotechnol Prog 15:147–157
Beg QK, Bhushan B, Kapoor M, Hoondal GS (2000) Enhanced production of a thermostable xylanase from Streptomyces sp. QG-11–3 and its application in biobleaching of eucalyptus kraft pulp. Enzyme Microb Technol 27:459–466
Sindhu I, Chhibber S, Capalash N, Sharma P (2006) Production of cellulase-free xylanase from Bacillus megaterium by solid state fermentation for biobleaching of pulp. Curr Microbiol 53:167–172
Rizzatti ACS, Jorge JA, Terenzi HF, Rechia CGV, Polizeli MLTM (2001) Purification and properties of a thermostable extracellular β-d-xylosidase produced by a thermotolerant Aspergillus phoenicis. J Ind Microbiol Biotechnol 4:265–275
Rizzatti ACS, Sandrim VC, Jorge JA, Terenzi HF, Polizeli MLTM (2004) Influence of temperature on the properties of xylanolytic enzymes of the thermotolerant fungus Aspergillus phoenicis. J Ind Microbiol Biotechnol 31:88–93
Sandrim VC, Rizzatti ACS, Terenzi HF, Jorge JA, Milagres AMF, Polizeli MLTM (2005) Purification and biochemical characterization of two xylanases produced by Aspergillus caespitosus and their potential for kraft pulp bleaching. Process Biochem 40:1823–1828
Dobrev GT, Pishtiyski IG, Stanchev VS, Mircheva R (2007) Optimization of nutrient medium containing agricultural wastes for xylanase production by Aspergillus niger B03 using optimal composite experimental design. Bioresour Technol 98:2671–2678
Chadha BS, Kanwar SS, Garcha HS (1995) Simultaneous saccharification and fermentation of rice straw into ethanol. Acta Microbiol Immunol Hung 42:71–75
Emerson R (1941) An experimental study of the life cycles and taxonomy of Allomyces. Lloydia 4:77–144
Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–429
McIlvaine TC (1921) A buffer solution for colorimetric comparison. J Biol Chem 49:183–186
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:267–275
TAPPI test methods (1996) Technical association of the pulp and paper industry. TAPPI Press, Atlanta
Ninawe S, Kuhad RC (2005) Use of xylan-rich cost effective agro-residues in the production of xylanase by Streptomyces cyaneus SN32. J Appl Microbiol 99:1141–1148
Poorna CA, Prema P (2007) Production of cellulase-free endoxylanase from novel alkalophilic thermotolerant Bacillus pumilus by solid-state fermentation and its application in wastepaper recycling. Bioresour Technol 98:485–490
Archana S, Sathyanarayana T (1997) Xylanase production by thermophilic Bacillus lichenformis A99 in solid-state fermentation. Enzyme Microb Technol 21:12–17
Lequart C, Nuzillard JM, Kurek B, Debeire P (1999) Hydrolysis of wheat bran and straw by an endoxylanase: production and structural characterization of cinnamoyl-oligosaccharides. Carbohydr Res 319:102–111
Babu KR, Satyanarayana T (1996) α-Amylase production by thermophilic Bacillus coagulans in solid state fermentation. Process Biochem 30:305–309
George SP, Ahmad A, Rao MB (2001) A novel thermostable xylanase from Thermomonospora sp: influence of additives on thermostability. Bioresour Technol 78:221–224
Acknowledgments
This work was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). J.A.J., H·F.T. and M.L.T.M.P. are Research Fellows of CNPq. This work was part of Master Dissertation of J.H.A.B. submitted to the Departamento de Biologia—FFCLRP/USP. The authors thank Ricardo F. Alarcon and Maurício de Oliveira for technical assistance.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Betini, J.H.A., Michelin, M., Peixoto-Nogueira, S.C. et al. Xylanases from Aspergillus niger, Aspergillus niveus and Aspergillus ochraceus produced under solid-state fermentation and their application in cellulose pulp bleaching. Bioprocess Biosyst Eng 32, 819–824 (2009). https://doi.org/10.1007/s00449-009-0308-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00449-009-0308-y