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Streptomyces sp. LK3 mediated synthesis of silver nanoparticles and its biomedical application

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Abstract

In the present study, the marine actinobacteria mediated biosynthesis of silver nanoparticles (AgNps) was achieved using Streptomyces sp LK3. The synthesized AgNps showed the characteristic absorption spectra in UV–vis at 420 nm, which confirmed the presence of nanoparticles. XRD analysis showed intense peaks at 2θ values of 27.51°, 31.87°, 45.57°, 56.56°, 66.26°, and 75.25° corresponding to (210), (113), (124), (240), (226), and (300) Bragg’s reflection based on the fcc structure of AgNps. The FTIR spectra exhibited prominent peaks at 3,417 cm−1 (OH stretching due to alcoholic group) and 1,578 cm−1 (C=C ring stretching). TEM micrograph showed that the synthesized AgNps were spherical in shape with an average size of 5 nm. Surface morphology and topographical structure of the synthesized AgNps were dignified by AFM. The synthesized AgNps showed significant acaricidal activity against Rhipicephalus microplus and Haemaphysalis bispinosa with LC50 values of 16.10 and 16.45 mg/L, respectively. Our results clearly indicate that AgNps could provide a safer alternative to conventional acaricidal agents in the form of a topical antiparasitic formulation. The present study aimed to develop a novel, cost-effective, eco-friendly actinobacteria mediated synthesis of AgNps and its antiparasitic activity.

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Acknowledgments

The authors wish to thank the Management of VIT University for providing necessary facilities to carry out this study.

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Authors and Affiliations

  1. Environmental Biotechnology Division, School of Bio Sciences and Technology, VIT University, Vellore, 632 014, Tamil Nadu, India

    L. Karthik, Gaurav Kumar & K. V. Bhaskara Rao

  2. Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam, Vellore, 632 509, Tamil Nadu, India

    A. Vishnu Kirthi & A. A. Rahuman

Authors
  1. L. Karthik
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  2. Gaurav Kumar
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  3. A. Vishnu Kirthi
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  4. A. A. Rahuman
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  5. K. V. Bhaskara Rao
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Correspondence to K. V. Bhaskara Rao.

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Karthik, L., Kumar, G., Kirthi, A.V. et al. Streptomyces sp. LK3 mediated synthesis of silver nanoparticles and its biomedical application. Bioprocess Biosyst Eng 37, 261–267 (2014). https://doi.org/10.1007/s00449-013-0994-3

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  • DOI: https://doi.org/10.1007/s00449-013-0994-3

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