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Electrospun polylactic acid and polyvinyl alcohol fibers as efficient and stable nanomaterials for immobilization of lipases

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Abstract

Electrospinning was applied to create easy-to-handle and high-surface-area membranes from continuous nanofibers of polyvinyl alcohol (PVA) or polylactic acid (PLA). Lipase PS from Burkholderia cepacia and Lipase B from Candida antarctica (CaLB) could be immobilized effectively by adsorption onto the fibrous material as well as by entrapment within the electrospun nanofibers. The biocatalytic performance of the resulting membrane biocatalysts was evaluated in the kinetic resolution of racemic 1-phenylethanol (rac-1) and 1-phenylethyl acetate (rac-2). Fine dispersion of the enzymes in the polymer matrix and large surface area of the nanofibers resulted in an enormous increase in the activity of the membrane biocatalyst compared to the non-immobilized crude powder forms of the lipases. PLA as fiber-forming polymer for lipase immobilization performed better than PVA in all aspects. Recycling studies with the various forms of electrospun membrane biocatalysts in ten cycles of the acylation and hydrolysis reactions indicated excellent stability of this forms of immobilized lipases. PLA-entrapped lipases could preserve lipase activity and enantiomer selectivity much better than the PVA-entrapped forms. The electrospun membrane forms of CaLB showed high mechanical stability in the repeated acylations and hydrolyses than commercial forms of CaLB immobilized on polyacrylamide beads (Novozyme 435 and IMMCALB-T2-150).

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Acknowledgments

The research was supported by the OTKA Research Fund (code 112644 and code 108975). Besides this project is supported by the New Széchenyi Plan (Project ID: TÁMOP-4.2.1/B-09/1/KMR-2010-0002), János Bolyai Research Scholarship of the Hungarian Academy of Sciences and MedInProt Project. The authors would like to express their special thanks to NanGenex Inc., (Budapest, Hungary) for DLS measurements.

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    Authors and Affiliations

    1. Department of Organic Chemistry and Technology, Budapest University of Technology and Economics, Budafoki út 8., 1111, Budapest, Hungary

      Péter Lajos Sóti, Diana Weiser, Tamás Vigh, Zsombor Kristóf Nagy, László Poppe & György Marosi

    2. SynBiocat Ltd., Lázár deák u. 4/1, 1173, Budapest, Hungary

      László Poppe

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    1. Péter Lajos Sóti
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    Correspondence to Péter Lajos Sóti.

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    L. Poppe and G. Marosi have contributed equally to this work.

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    Sóti, P.L., Weiser, D., Vigh, T. et al. Electrospun polylactic acid and polyvinyl alcohol fibers as efficient and stable nanomaterials for immobilization of lipases. Bioprocess Biosyst Eng 39, 449–459 (2016). https://doi.org/10.1007/s00449-015-1528-y

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