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Immobilization of urease on vermiculite

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Abstract

Urease (EC 3.5.1.5) of high activity was obtained when the enzyme was immobilized on vermiculite crosslinked with 2.5% glutaraldehyde in chilled EDTA-phosphate buffer (pH 5.5). The highest activity of the immobilized enzyme was at 65°C and pH 6.5 while the optimum temperature for free urease was found to be 25°C. The thermal stability of immobilized urease was observed to be much better than that of the free urease. When stored at 4°C, urease immobilized on vermiculite retained 69 to 81% of its activity after 60 days and 61 to 75% of its original activity was retained after 4 repeated uses.

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Authors and Affiliations

  1. Institute of Advanced Studies, University of Malaya, 50603 Kuala Lumpur, Malaysia, , , , , , MY

    A. Anita, C. A. Sastry & Prof. Dr. M. A. Hashim

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  1. A. Anita
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  2. C. A. Sastry
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  3. Prof. Dr. M. A. Hashim
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Received: 22 July 1996

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Anita, A., Sastry, C. & Hashim, P. Immobilization of urease on vermiculite. Bioprocess Engineering 16, 375–380 (1997). https://doi.org/10.1007/s004490050338

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  • DOI: https://doi.org/10.1007/s004490050338

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