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Effects of ozone on nitrogen metabolism in the leaves of Fagus crenata seedlings under different soil nitrogen loads

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Abstract

To clarify the effects of ozone (O3) on nitrogen (N) metabolism in the leaves of Fagus crenata seedlings under different N loads, the combined effects of O3 and N load on N enzyme activity, amino acid profiles and soluble protein concentrations were investigated. The seedlings were grown in potted andisol supplied with N at 0 (N0), 20 (N20) and 50 kg ha−1 year−1 (N50) and were exposed to charcoal-filtered air or O3 at 1.0, 1.5 and 2.0 times the ambient concentration in open-top chambers from April 2004 to October 2005. The average 24-h concentrations of O3 during the two growing seasons were 11.8, 42.7, 63.3 and 83.7 ppb, respectively. In July 2005, exposure to O3 did not significantly affect the concentration of total free amino acids and activities of nitrite reductase and glutamine synthetase in any N treatment. Exposure to O3 significantly increased the relative content of acidic amino acids in all N treatments while it significantly reduced concentration of total soluble protein (TSP) and ratio of TSP concentration to leaf N concentration in the N50 treatment, but not in the N0 and N20 treatments. Based on the results obtained in the present study, we concluded that exposure to O3 reduces the allocation of N to soluble protein in addition to O3-induced degradation of protein in the leaves of seedlings grown under a relatively high N load, with the result that the degree of O3-induced reduction in the soluble protein was greater under a relatively high N load than under a relatively low N load.

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Acknowledgments

This study was financially supported by a grant from the Ministry of the Environment, Japan through the program of Global Environment Research Fund (C-03-07, 2003–2005). The authors greatly acknowledge Prof. Hisao Itabashi and Prof. Keiji Hasumi of Tokyo University of Agriculture and Technology for their valuable advice on the analysis of amino acids. The authors are greatly indebted to Prof. Motohiro Fukami of Utsunomiya University and Prof. Ryo Funada of Tokyo University of Agriculture and Technology for their invaluable advice. We thank Mayumi Iwasaki, Chikako Tabe, Junichi Naba, Naoki Matsuo, Rie Yamashita, Yusuke Shinozaki and Masamori Tanaka for their technical supports and discussions.

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Author notes

  1. Masahiro Yamaguchi

    Present address: Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan

  2. Makoto Watanabe

    Present address: JSPS Research Fellowship, Research Faculty of Agriculture, Hokkaido University, Sapporo, 060-8589, Japan

Authors and Affiliations

  1. United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan

    Masahiro Yamaguchi & Makoto Watanabe

  2. Central Research Institute of Electric Power Industry, Abiko, Chiba, 270-1194, Japan

    Hideyuki Matsumura & Yoshihisa Kohno

  3. Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan

    Takeshi Izuta

Authors
  1. Masahiro Yamaguchi
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  2. Makoto Watanabe
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  3. Hideyuki Matsumura
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  4. Yoshihisa Kohno
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  5. Takeshi Izuta
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Correspondence to Takeshi Izuta.

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Communicated by T. Grams.

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Yamaguchi, M., Watanabe, M., Matsumura, H. et al. Effects of ozone on nitrogen metabolism in the leaves of Fagus crenata seedlings under different soil nitrogen loads. Trees 24, 175–184 (2010). https://doi.org/10.1007/s00468-009-0391-3

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  • DOI: https://doi.org/10.1007/s00468-009-0391-3

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