Skip to main content

Advertisement

SpringerLink
Log in

Development of polymer-modified magnetic nanoparticles and quantum dots for Escherichia coli binding test

  • Original Paper
  • Published:
Microchimica Acta Aims and scope Submit manuscript

Abstract

A rapid binding test has been developed for the detection of bacteria using polymer-modified magnetic nanoparticles. Polydopamine (PDA) can effectively act as a sorbent even in water solution, and a PDA coating on magnetic nanoparticles (MNPs) was therefore prepared to bind Escherichia coli (E. coli). Albeit non-selective, PDA-modified magnetic nanoparticles (MNPs@PDA) show nearly 100% efficiency in binding E. coli. If E. coli, grown in tryptic soy broth medium, is analyzed by capillary electrophoresis (CE) using phosphate buffer as the background electrolyte, two peaks are found, while a single peak is found with carbonate buffer containing 0.05% of poly(ethylene glycol). Self-polymerization of dopamine on E. coli at pH 9.5 is also feasible. The detection of E. coli is demonstrated by adding quantum dots (QDs) to form a QDs-PDA-E. coli aggregate for better CE analysis.

Development of Polymer-Modified Magnetic Nanoparticles and Quantum Dots for Escherichia coli Binding Test

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Feng P, Weagant S, Grant M (2002) Enumeration of Escherichia coli and the Coliform Bacteria. Bacteriological Analytical Manual (8th ed.). FDA/Center for Food Safety & Applied Nutrition. Sept 2002. Retrieved 2007-01-25. http://www.cfsan.fda.gov/~ebam/bam-4.html

  2. Kung’u JK, Boor KJ, Ame SM, Ali NS, Jackson AE, Stoltzfus RJ (2009) Bacterial populations in complementary foods and drinking-water in households with children aged 10–15 months in Zanzibar, Tanzania. J Health Popul Nutr 27:41–52. PMCID: PMC2761806

    Google Scholar 

  3. Nigam S, Barick KC, Bahadur D (2011) Development of citrate-stabilized Fe3O4 nanoparticles: conjugation and release of doxorubicin for therapeutic applications. J Magn Magn Mater 323:237–243. doi:10.1016/j.jmmm.2010.09.009

    Article  CAS  Google Scholar 

  4. Yang K, Su WW (2011) Facile synthesis of metal-chelating magnetic nanoparticles by exploiting organophosphorus coupling. Anal Biochem 408:175–177. doi:10.1016/j.ab.2010.09.014

    Article  CAS  Google Scholar 

  5. Forge D, Laurent S, Gossuin Y, Roch A, Vander EL, Muller RN (2011) An original route to stabilize and functionalize magnetite nanoparticles for theranosis applications. J Magn Magn Mater 323:410–415. doi:10.1016/j.jmmm.2010.09.031

    Article  CAS  Google Scholar 

  6. Sima VH, Patris S, Aydogmus Z, Sarakbi A, Sandulescu R, Kauffmann JM (2011) Tyrosinase immobilized magnetic nanobeads for the amperometric assay of enzyme inhibitors: application to the skin whitening agents. Talanta 83:980–987. doi:10.1016/j.talanta.2010.11.005

    Article  CAS  Google Scholar 

  7. Hnaiein M, Hassen WM, Abdelghani A, Fournier WC, Coste JBF, Leonard D, Jaffrezic RN (2008) A conductometric immunosensor based on functionalized magnetic nanoparticles for E. coli detection. Electrochem Commun 10:1152–1154. doi:10.1016/j.elecom.2008.04.009

    Article  CAS  Google Scholar 

  8. Wan Y, Zhang D, Wang Y, Qi P, Hou B (2011) Direct immobilisation of antibodies on a bioinspired architecture as a sensing platform. Biosens Bioelectron 26:2595–2600. doi:10.1016/j.bios.2010.11.013

    Article  CAS  Google Scholar 

  9. Ou J, Wang J, Zhou J, Liu S, Yu Y, Pang X, Yang S (2010) Construction and study on corrosion protective property of polydopamine-based 3-layer organic coatings on aluminum substrate. Prog Org Coat 68:244–247. doi:10.1016/j.porgcoat.2010.01.004

    Article  CAS  Google Scholar 

  10. McCloskey BD, Park HB, Ju H, Rowe BW, Miller DJ, Chun BJ, Kin K, Freeman BD (2010) Influence of polydopamine deposition conditions on pure water flux and foulant adhesion resistance of reverse osmosis, ultrafiltration, and microfiltration membranes. Polymer 51:3472–3485. doi:10.1016/j.polymer.2010.05.008

    Article  CAS  Google Scholar 

  11. Ouyang R, Lei J, Ju H (2010) Artificial receptor-functionalized nanoshell: facile preparation, fast separation and specific protein recognition. Nanotechnology 21:185502. Epub 2010 April 14. PMID:20388981

    Google Scholar 

  12. Iqbal Z, Alsudir S, Miah M, Lai EPC (2011) Rapid CE-UV binding tests of environmentally hazardous compounds with polymer-modified magnetic nanoparticles. Electrophoresis 32:1–7. doi:10.1002/elps.201100106

    Article  Google Scholar 

  13. Chen P, Li RK, Xu XH, Rao PF (2002) Rapid separation and determination of enterotoxigenic E. coli by capillary zone electrophoresis. Chin J Chromatogr 20:39–41. doi:1000 8713(2002) 05 0439 03

    Google Scholar 

  14. Igor VK, Mirjana RP, Edward D, Carmel GR, Andreas K, Annelise EB (2003) Detection of Escherichia coli O157:H7 bacteria by a combination of immunofluorescent staining and capillary electrophoresis. Electrophoresis 24:655–661. doi:10.1002/elps.200390077

    Article  Google Scholar 

  15. Law WS, Li SFY, Kricka LJ (2009) Microchip Methods in Diagnostics Series. In: Bilitewski U (ed) Methods in Molecular Biology. Chapter 12, pp169-179, Humana Press, USA. Pub. Date: Apr-01-2008. doi: 10.1007/978-1-59745-372-1_12

  16. Mukhopadhyay B, Martins MB, Karamanska R, Russell DA, Field RA (2009) Bacterial detection using carbohydrate-functionalised CdS quantum dots: a model study exploiting E. coli recognition of mannosides. Tetrahedron Lett 50:886–889. doi:10.1016/j.tetlet.2008.12.029

    Article  CAS  Google Scholar 

  17. Carrillo CC, Simonet BM, Valcárcel M (2011) Colistin-functionalised CdSe/ZnS quantum dots as fluorescent probe for the rapid detection of Escherichia coli. Biosens Bioelectron 26:4368–4374. doi:10.1016/j.bios.2011.04.050

    Article  Google Scholar 

  18. Wen HZ, Chun HL, Xiu CG, Fa RC, Huang HY, Xiao RW (2010) Mussel-inspired molecularly imprinted polymer coating superparamagnetic nanoparticles for protein recognition. J Mater Chem 20:880–883. doi:10.1039/b916619j

    Article  Google Scholar 

  19. Roy H (2009) Tuning the properties of the bacterial membrane with aminoacylated phosphatidylglycerol. IUBMB Life 61:940–953. PMCID: PMC2757517

    Google Scholar 

  20. Liu A, Zhao L, Bai H, Zhao H, Xing X, Shi G (2009) Polypyrrole actuator with a bioadhesive surface for accumulating bacteria from physiological media. ACS Appl Mater Interfaces 1:951–955

    Article  CAS  Google Scholar 

  21. Sureshkumar M, Lee CK (2011) Polydopamine coated magnetic-chitin (MCT) particles as a new matrix for enzyme immobilization. Carbohydr Polym 84:775–780. doi:10.1016/j.carbpol.2010.03.036

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors thank Laura Novitsky for technical assistance. This work was supported by Natural Sciences and Engineering Research Council of Canada (NSERC) fund 315574 to EPCL, and Bangladesh Agricultural University study leave to ZI.

Author information

Authors and Affiliations

  1. Department of Chemistry, Carleton University, 1125 Colonel By Drive, Ottawa, ON, K1S 5B6, Canada

    Zafar Iqbal, Edward P. C. Lai & Tyler J. Avis

Authors
  1. Zafar Iqbal
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Edward P. C. Lai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tyler J. Avis
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Edward P. C. Lai.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Esm 1

(DOC 494 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Iqbal, Z., Lai, E.P.C. & Avis, T.J. Development of polymer-modified magnetic nanoparticles and quantum dots for Escherichia coli binding test. Microchim Acta 176, 193–200 (2012). https://doi.org/10.1007/s00604-011-0712-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00604-011-0712-2

Keywords

Search

Navigation