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Comparative proteome analysis of drought-sensitive and drought-tolerant rapeseed roots and their hybrid F1 line under drought stress

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Abstract

Rapeseed (Brassica napus L.), which is the third leading source of vegetable oil, is sensitive to drought stress during the early vegetative growth stage. To investigate the initial response of rapeseed to drought stress, changes in the protein expression profiles of drought-sensitive (RGS-003) and drought-tolerant lines (SLM-003), and their F1 hybrid, were analyzed using a proteomics approach. Seven-day-old rapeseed seedlings were treated with drought stress by restricting water for 7 days, and proteins were extracted from roots and separated by two-dimensional polyacrylamide gel electrophoresis. In the sensitive rapeseed line, 35 protein spots were differentially expressed under drought stress, and proteins related to metabolism, energy, disease/defense, and transport were decreased. In the tolerant line, 32 protein spots were differentially expressed under drought stress, and proteins involved in metabolism, disease/defense, and transport were increased, while energy-related proteins were decreased. Six protein spots in F1 hybrid were common among expressed proteins in the drought-sensitive and -tolerant lines. Notably, tubulin beta-2 and heat shock protein 70 were decreased in the drought-sensitive line and hybrid F1 plants, while jasmonate-inducible protein and 20S proteasome subunit PAF1 were increased in the F1 hybrids and drought-tolerant line. These results indicate that (1) V-type H+ ATPase, plasma-membrane associated cation-binding protein, HSP 90, and elongation factor EF-2 have a role in the drought tolerance of rapeseed; (2) The decreased levels of heat shock protein 70 and tubulin beta-2 in the drought-sensitive and hybrid F1 lines might explain the reduced growth of these lines in drought conditions.

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Abbreviations

2-DE:

Two-dimensional polyacrylamide gel electrophoresis

CBB:

Coomassie brilliant blue

MS:

Mass spectrometry

LC:

Liquid column

pI:

Isoelectric point

IEF:

Isoelectric focusing

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Acknowledgments

The authors thank Dr. Yohei Nanjo and Dr. Keito Nishazawa for their valuable discussion.

Conflict of interest

This work was supported by the Scholarship Section of the Ministry of Science, Research and Technology and Tarbiat Modares University of I. R. Iran and National Institute of Crop Science of Japan.

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Authors and Affiliations

  1. National Institute of Crop Science, 2-1-18 Kannondai, Tsukuba, 305-8518, Japan

    Payam Pour Mohammadi & Setsuko Komatsu

  2. Faculty of Agriculture, Department of Biotechnology and Plant Breeding, Tarbiat Modares University, 14115-111, Tehran, Iran

    Payam Pour Mohammadi & Ahmad Moieni

Authors
  1. Payam Pour Mohammadi
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  2. Ahmad Moieni
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  3. Setsuko Komatsu
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Correspondence to Setsuko Komatsu.

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726_2012_1299_MOESM1_ESM.pptx

Effect of drought stress on rapeseed roots, hypocotyls, and leaves. Seven-day-old rapeseed seedlings were treated without (white) or with (black) drought stress for 1, 2, 3, 4, 5, 6, and 7 days. Leaf weight, hypocotyl weight, hypocotyl length, and root number were measured daily. Three rapeseed plants were used for each experiment and three independent experiments were performed. Values are presented as the mean ± SE and were compared using the Student t test (no significant changes were detected) (PPTX 90 kb)

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Mohammadi, P.P., Moieni, A. & Komatsu, S. Comparative proteome analysis of drought-sensitive and drought-tolerant rapeseed roots and their hybrid F1 line under drought stress. Amino Acids 43, 2137–2152 (2012). https://doi.org/10.1007/s00726-012-1299-6

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  • DOI: https://doi.org/10.1007/s00726-012-1299-6

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