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Thermodynamic description of retention mechanism in liquid chromatography

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Abstract

The chemical and physical properties of the stationary and the mobile phase determine the retention and selectivity of the separated molecules in chromatographic process. It should explain the specific and non-specific interactions in the chromatographic system. Special emphasis should be placed on column selection through the adequate choice of column dimensions (diameter and length) and the type of stationary phase with required parameters, which contributes the system effectiveness. However, column effectiveness is unable to guarantee a satisfactory level of substance separation. For this reason, the interactions between the analyte, the stationary and the mobile phase need to be investigated to determine the retention factors of the separated substances and the optimal parameters of the chromatographic system. According to the principles of thermodynamics, in particular molecular interactions, the chromatographic process has to be carried out with a high level of control. This work can be treated a brief tutorial devoted to thermodynamics of liquid chromatography process with a special emphasis on molecular interactions between analyte species and the components of the mobile and stationary phases.

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Acknowledgments

One from us (BB) thanks to Foundation for Polish Science for Mistrz grant.

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Authors and Affiliations

  1. Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarin St., 87 100, Toruń, Poland

    Bogusław Buszewski & Renata Gadzała-Kopciuch

  2. Plant Protection Institute-NRI, 20 Węgorka St., 60-318, Poznan, Poland

    Monika Michel

Authors
  1. Bogusław Buszewski
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  2. Renata Gadzała-Kopciuch
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  3. Monika Michel
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Corresponding author

Correspondence to Bogusław Buszewski.

Additional information

This article is part of the Topical Issue "Quality Assurance of Thermodynamic Data".

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Buszewski, B., Gadzała-Kopciuch, R. & Michel, M. Thermodynamic description of retention mechanism in liquid chromatography. Accred Qual Assur 16, 237–244 (2011). https://doi.org/10.1007/s00769-011-0761-6

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  • DOI: https://doi.org/10.1007/s00769-011-0761-6

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