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Halobacillus locisalis sp. nov., a halophilic bacterium isolated from a marine solar saltern of the Yellow Sea in Korea

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Abstract

A Gram-positive, motile, endospore-forming and rod-shaped halophilic bacterial strain MSS-155 (KCTC 3788 and KCCM 41687) was isolated from a marine solar saltern of the Yellow Sea in Korea and was subjected to a polyphasic taxonomic study. This organism grew at temperature of 10.0–42.0°C with an optimum of 35°C. Strain MSS-155 grew optimally in the presence of 10% NaCl and did not grow in the absence of NaCl. The cell wall peptidoglycan type of strain MSS-155 was A4β based on l-Orn-d-Asp. Strain MSS-155 was also characterized chemotaxonomically by having menaquinone-7 (MK-7) as the predominant isoprenoid quinone and anteiso-C15:0 as the major fatty acid. The DNA G+C content was 44.0 mol%. Phylogenetic analysis based on 16S rDNA sequences showed that strain MSS-155 falls within the radiation of the cluster comprising Halobacillus species. Levels of 16S rDNA sequence similarity between strain MSS-155 and the type strains of four Halobacillus species were in the range 97.6–98.8%. Strain MSS-155 exhibited levels of DNA-DNA relatedness of 6.2–11.2% to the type strains of Halobacillus species described previously. On the basis of phenotypic properties, phylogeny, and genomic data, strain MSS-155 should be placed in the genus Halobacillus as a member of a novel species, for which we propose the name Halobacillus locisalis sp. nov.

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Acknowledgments

This work was supported by the 21C Frontier program of Microbial Genomics and Applications (grant MG02-0401-001-1-0-0), the NRL research program (grants M10104000294-01J000012800 and M10104000294-01J000012811) of the Ministry of Science and Technology (MOST) of the Republic of Korea, and by the research fund of Probionic Corporation of Korea.

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Authors and Affiliations

  1. Korea Research Institute of Bioscience and Biotechnology (KRIBB), P.O. Box 115, Yusong, Taejon, Korea

    Jung-Hoon Yoon, Tae-Kwang Oh & Yong-Ha Park

  2. Department of Food and Life Science, Sungkyunkwan University, Chunchun-dong 300, Jangan-gu, Suwon, Korea

    Kook Hee Kang

  3. National Research Laboratory of Molecular Ecosystematics, Institute of Probionic, Probionic Corporation, Bio-venture Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), P.O. Box 115, Yusong, TaejonKorea

    Yong-Ha Park

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  1. Jung-Hoon Yoon
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  2. Kook Hee Kang
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Correspondence to Jung-Hoon Yoon.

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Communicated by W.D. Grant

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Yoon, JH., Kang, K.H., Oh, TK. et al. Halobacillus locisalis sp. nov., a halophilic bacterium isolated from a marine solar saltern of the Yellow Sea in Korea. Extremophiles 8, 23–28 (2004). https://doi.org/10.1007/s00792-003-0352-5

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  • DOI: https://doi.org/10.1007/s00792-003-0352-5

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