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Dethiobacter alkaliphilus gen. nov. sp. nov., and Desulfurivibrio alkaliphilus gen. nov. sp. nov.: two novel representatives of reductive sulfur cycle from soda lakes

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Abstract

Anaerobic enrichments with H2 as electron donor and thiosulfate/polysulfide as electron acceptor at pH 10 and 0.6 M total Na+ yielded two non sulfate-reducing representatives of reductive sulfur cycle from soda lake sediments. Strain AHT 1 was isolated with thiosulfate as the electron acceptor from north–eastern Mongolian soda lakes and strain AHT 2—with polysulfide as the electron acceptor from Wadi al Natrun lakes in Egypt. Both isolates represented new phylogenetic lineages: AHT 1—within Clostridiales and AHT 2—within the Deltaproteobacteria. Both bacteria are obligate anaerobes with respiratory metabolism. Both grew chemolithoautotrophically with H2 as the electron donor and can use thiosulfate, elemental sulfur and polysulfide as the electron acceptors. AHT 2 also used nitrate as acceptor, reducing it to ammonia. During thiosulfate reduction, AHT 1 excreted sulfite. dsrAB gene was not found in either strain. Both strains were moderate salt-tolerant (grow up to 2 M total Na+) true alkaliphiles (grow between pH 8.5 and 10.3). On the basis of the phenotypic and phylogenetic data, strains AHT 1 and AHT 2 are proposed as new genera and species Dethiobacter alkaliphilus and Desulfurivibrio alkaliphilus, respectively.

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Acknowledgments

This work was supported by NWO-RFBR (47.011.2004.010), RFBR (07-04-00153) and by the Program of the Russian Academy of Sciences “Molecular and Cell Biology”.

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Authors and Affiliations

  1. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-let Octyabrya 7/2, 117811, Moscow, Russia

    D. Yu. Sorokin & T. P. Tourova

  2. Environmental Biotechnology Group, Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands

    D. Yu. Sorokin & G. Muyzer

  3. Department of Microbial Ecology, University of Vienna, Althanstraße 14, 1090, Vienna, Austria

    Marc Mußmann

Authors
  1. D. Yu. Sorokin
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  2. T. P. Tourova
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  3. Marc Mußmann
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  4. G. Muyzer
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Corresponding author

Correspondence to D. Yu. Sorokin.

Additional information

Communicated by K. Horikoshi.

Nucleotide sequence accession number: The GenBank/EMBL accession number of the 16S rRNA gene sequence of strains AHT 1T and AHT 2T are EF422412 and EF422413.

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Sorokin, D.Y., Tourova, T.P., Mußmann, M. et al. Dethiobacter alkaliphilus gen. nov. sp. nov., and Desulfurivibrio alkaliphilus gen. nov. sp. nov.: two novel representatives of reductive sulfur cycle from soda lakes. Extremophiles 12, 431–439 (2008). https://doi.org/10.1007/s00792-008-0148-8

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  • DOI: https://doi.org/10.1007/s00792-008-0148-8

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