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Poly(pyrrole) versus poly(3,4-ethylenedioxythiophene): amperometric cholesterol biosensor matrices

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Abstract

Two conducting polymers, poly(pyrrole) (PPy) and poly(3,4-ethylenedioxythiophene) (PEDOT) were used as immobilization matrices for cholesterol oxidase (ChOx). The amperometric responses of the enzyme electrodes were measured by monitoring oxidation current of H2O2 at +0.7 V in the absence of a mediator. Kinetic parameters, such as K m and I max, operational and storage stabilities, effects of pH and temperature were determined for both entrapment supports. K m values are found as 7.9 and 1.3 mM for PPy and PEDOT enzyme electrodes, respectively; it can be interpreted that ChOx immobilized in PEDOT shows higher affinity towards the substrate.

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Abbreviations

Py:

pyrrole

PPy:

poly(pyrrole)

EDOT:

3,4-ethylenedioxy thiophene

PEDOT:

poly(3,4-ethylenedioxythiophene)

ChOx:

cholesterol oxidase

References

  1. Madder S (1996) Biology, 5th edn. Times Mirror Higher Education Group, Inc., USA, pp 51, 86, 87, 638, 639

  2. Allain CC, Poon LS, Chan CSG, Richmond W, Fu PC (1974) Clin Chem 20:470

    CAS  Google Scholar 

  3. Sadana A (2001) Engineering biosensors: kinetics and design applications, 1st edn. Academic, USA, pp 1

    Google Scholar 

  4. Çırpan A, Alkan S, Toppare L, Cianga I, Yağcı Y (2003) Des Monomers Polym 6:237

    Article  Google Scholar 

  5. Kumar A, Pandey RR, Brantley B (2006) Talanta 69:700

    Article  CAS  Google Scholar 

  6. Singh S, Chaubey A, Malhotra BD (2003) J Appl Polym Sci 91:3796

    Google Scholar 

  7. Arya SK, Solanki PR, Singh SP, Kaneto K, Pandey MK, Datta M, Malhotra BD (2007) Biosens Bioelectron 22:2516

    Article  CAS  Google Scholar 

  8. Arya SK, Solanki PR, Singh RP, Pandey MK, Datta M, Malhotra BD (2006) Talanta 69:918

    Article  CAS  Google Scholar 

  9. Arya SK, Prusty AK, Singh SP, Solanki PR, Pandey MK, Datta M, Malhotra BD (2007) Anal Biochem 363:210

    Article  CAS  Google Scholar 

  10. Solanki PR, Arya SK, Nishimura Y, Iwamoto M, Malhotra BD (2007) Langmuir 23:7398

    Article  CAS  Google Scholar 

  11. Chong KT, Su X, Lee EJD, O’Shea SJ (2002) Langmuir 18:9932

    Article  CAS  Google Scholar 

  12. Özer BC, Özyörük H, Çelebi SS, Yıldız A (2007) Enzyme Microb Technol 40:262

    Article  Google Scholar 

  13. Devadoss A, Palencsar MS, Jiang D, Honkonen ML, Burgess JD (2005) Anal Chem 77:7393

    Article  CAS  Google Scholar 

  14. Vidal JC, Espuelas J, Castillo JR (2004) Anal Biochem 333:88

    Article  CAS  Google Scholar 

  15. Bakker E, Qin Y (2006) Anal Chem 78:3965

    Article  CAS  Google Scholar 

  16. Zhou N, Wang J, Chen T, Yu Z, Li G (2006) Anal Chem 78:5227

    Article  CAS  Google Scholar 

  17. Tan X, Li M, Cai P, Luo L, Zou X (2005) Anal Biochem 337:111

    Article  CAS  Google Scholar 

  18. Singh S, Chaubey A, Malhotra BD (2004) Anal Chim Acta 502:229

    Article  CAS  Google Scholar 

  19. Vidal JC, Espuelas J, Garcia-Ruiz E, Castillo JR (2004) Talanta 64:655

    Article  CAS  Google Scholar 

  20. Brahim S, Narinesingh D, Guiseppi-Elie A (2001) Anal Chim Acta 448:27

    Article  CAS  Google Scholar 

  21. Garcia-Ruiz E, Vidal JC, Aramendia MT, Castillo JR (2004) Electroanal 16:497

    Article  CAS  Google Scholar 

  22. Matharu Z, Sumana G, Arya SK, Singh SP, Gupta V, Malhotra BD (2007) Langmuir 23:13188

    Article  CAS  Google Scholar 

  23. Cosnier S (2005) Electroanal 17:1701

    Article  CAS  Google Scholar 

  24. Singh S, Solanki PR, Pandey MK, Malhotra BD (2006) Anal Chim Acta 568:126

    Article  CAS  Google Scholar 

  25. Kros A, Sommerdijk NAJM, Nolte RJM (2005) Sensor Actuator B 106:289

    Article  Google Scholar 

  26. Argun AA, Aubert PH, Thopson BC, Schwendeman I, Gaupp CL, Hwang J, Pinto NJ, Tanner DB, MacDiarmid AG, Reynolds JR (2004) Chem Mater 16:4401

    Article  CAS  Google Scholar 

  27. Sotzing GA, Reddinger JL, Reynolds JR (1997) Synth Met 84:199

    Article  CAS  Google Scholar 

  28. Kros A, Van Hövell SWFM, Sommerdijk NAJM, Nolte RJM (2001) Adv Mater 13:1555

    Article  CAS  Google Scholar 

  29. Fabiano S, Tran-Minh C, Piro B, Dang LA, Pham MC, Vittori O (2002) Mater Sci Eng 21:61

    Article  Google Scholar 

  30. Nien PC, Tung TS, Ho KC (2001) Electroanal 18:1408

    Article  Google Scholar 

  31. Vedrine C, Fabiano S, Tran-Mink C (2003) Talanta 59:535

    Article  CAS  Google Scholar 

  32. Vidal JC, Garcia E, Castillo JR (2002) Anal Sci 18:537

    Article  CAS  Google Scholar 

  33. Jia F, Yu C, Gong J, Zhang L (2008) J Solid State Electrochem DOI 10.1007/s10008-008-0521-7

  34. Pauluikaite R, Ghica ME, Barsan M, Brett CMA (2007) J Solid State Electrochem 11:899

    Article  Google Scholar 

  35. Tan X, Li M, Luo L, Zou X (2005) Anal Biochem 337:111

    Article  CAS  Google Scholar 

  36. Marangoni AG (2003) Enzyme kinetics: a modern approach. Wiley, USA, pp 44–60

    Google Scholar 

  37. Mignani A, Luciano G, Lanteri S, Leardi R, Scavetta E, Tonelli D (2007) Anal Chim Acta 599:36

    Article  CAS  Google Scholar 

  38. Niculescu M, Erichsen T, Sukharev V, Kerenyi Z, Csöregi E, Schuhmann W (2002) Anal Chim Acta 463:39

    Article  CAS  Google Scholar 

  39. Lundgren JS, Bright FV (1996) Anal Chem 12:3377

    Article  Google Scholar 

  40. Thomas CA, Zong K, Schottland P, Reynolds JR (2000) Adv Mater 12:222

    Article  CAS  Google Scholar 

  41. Arslan A, Kıralp S, Toppare L, Bozkurt A (2006) Langmuir 22:2912

    Article  CAS  Google Scholar 

  42. MacLachlan J, Wotherspoon ATL, Ansell RO, Brooks CJW (2000) J Steroid Biochem 72:169

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors gratefully thank to DPT 2005K 120580, TUBITAK 105T385 and BAP-2007-01-03-03 and TUBA grants.

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Authors and Affiliations

  1. Department of Chemistry, Middle East Technical University, 06531, Ankara, Turkey

    Özlem Türkarslan, Senem Kıralp Kayahan & Levent Toppare

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  1. Özlem Türkarslan
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  2. Senem Kıralp Kayahan
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  3. Levent Toppare
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Correspondence to Levent Toppare.

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Türkarslan, Ö., Kayahan, S.K. & Toppare, L. Poly(pyrrole) versus poly(3,4-ethylenedioxythiophene): amperometric cholesterol biosensor matrices. J Solid State Electrochem 13, 657–663 (2009). https://doi.org/10.1007/s10008-008-0590-7

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