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Sensitive detection of biomolecules and DNA bases based on graphene nanosheets

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Abstract

High surface area electrode materials are of interest for the application of electrochemical sensors. Currently, chemical vapor deposition (CVD) graphene-sensing electrodes are scarce. Herein, for the first time, a graphene based on a Ta wire support was prepared using the CVD method to form a highly electroactive biosensing platform. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and differential pulse voltammetry (DPV) were utilized to characterize the morphology and investigate the electrochemical properties of the CVD graphene electrodes. The resulting CVD graphene electrode exhibited good electrocatalytic activity and had a prominent response effect on dopamine, uric acid, guanine, and adenine. Standing graphene nanosheets have rich catalytic sites such as the edges, the defect levels of the plane, and porous network structures between the graphene nanosheets. These catalytic sites prompt the adsorption and resolution for the four species and the strong electron transport capability of the CVD graphene, which effectively improved the electrical signals for response to four species. Moreover, the graphene electrode is a promising candidate in electrochemical sensing and other electrochemical device applications.

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Acknowledgments

This work was supported by the National key R&D program (No. 2016YFB0402703), the National Nature Science Foundation of China (Nos. 61301045, 61401306, and 61504096), the Natural Science Foundation of Tianjin City (Nos. 13JCZDJC36000, 15JCYBJC24000, 16JCTPJC50800, and 16JCYBJC16300), the Excellent Young Teachers Program of Tianjin, and the Youth Top-notch Talents Program of Tianjin.

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Authors and Affiliations

  1. Tianjin Key Laboratory of Film Electronic and Communicate Devices, School of Electronics Information Engineering, Tianjin University of Technology, No. 391, Extension of Bin Shui West Road, Xi Qing District, Tianjin, 300384, People’s Republic of China

    Delan Gao, Mingji Li, Cuiping Li, Ning Zhu & Baohe Yang

  2. Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, School of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin, 300384, People’s Republic of China

    Hongji Li

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  1. Delan Gao
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  2. Mingji Li
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  3. Hongji Li
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  5. Ning Zhu
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  6. Baohe Yang
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Correspondence to Mingji Li or Hongji Li.

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Gao, D., Li, M., Li, H. et al. Sensitive detection of biomolecules and DNA bases based on graphene nanosheets. J Solid State Electrochem 21, 813–821 (2017). https://doi.org/10.1007/s10008-016-3423-0

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  • DOI: https://doi.org/10.1007/s10008-016-3423-0

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