Skip to main content

Advertisement

SpringerLink
Log in

Preparation of carboxylated Ag nanoparticles as a coating material for medical devices and control of antibacterial activity

  • Original Article
  • Published:
Journal of Artificial Organs Aims and scope Submit manuscript

Abstract

Carboxyl group-donated silver (Ag) nanoparticles for coating on medical devices were prepared by a two-phase reduction system in situ. AgNO3 was the Ag ion source, tetraoctylammonium bromide [N(C8H17)4Br] the phase-transfer agent, sodium tetrahydroborate (NaBH4) the reducing agent and 10-carboxy-1-decanthiol (C11H22O2S, CDT) the capping agent. The characterizations of the Ag nanoparticles were conducted by diffuse reflectance Fourier transform infrared (FT-IR) spectroscopy, thermogravimetric differential thermal analysis (TG/DTA) and transmission electron microscope. With CDT capped on Ag nanoparticles, we found that the band around 3,100 cm−1 was attributed to COO-H stretching vibration, two adsorptions at 2,928 and 2,856 cm−1 to C–H symmetric/anti-symmetric stretching vibration, and at 1,718 cm−1 to C=O stretching vibration in the FT-IR spectra. The organic components of the carboxylated Ag nanoparticles were 5.8–25.9 wt%, determined by TG/DTA. The particle sizes of the carboxylated Ag nanoparticles were well controlled by the addition of the capping agent, CDT, into the reaction system. The antimicrobial activity of the Ag nanoparticles covered with different contents of CDT against E. coli was evaluated. Smaller-size Ag nanoparticles showed higher antibacterial activity, which depended on a surface area that attached easily to a microorganism cell membrane.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Sharma VK, Yngard RA, Lin Y. Silver nanoparticles: green synthesis and their antimicrobial activities. Adv Coll Int Sci. 2009;145:83–96.

    Article  CAS  Google Scholar 

  2. Rai M, Yadav A, Gade A. Silver nanoparticles as a new generation of antimicrobials. Biotech Adv. 2009;27:76–83.

    Article  CAS  Google Scholar 

  3. Knetsch MLW, Koole LH. New strategies in the development of antimicrobial coatings: The example of increasing usage of silver and silver nanoparticles. Polymers. 2011;3: 340–366. (OPEN ACCESS, ISSN 2073-4360).

    Google Scholar 

  4. Wong KKY, Liu X. Silver nanoparticles—the real “silver bullet” in clinical medicine? Med Chem Commun. 2010;1:125–31.

    Article  CAS  Google Scholar 

  5. Marambio-Jones C, Hoek EMV. A review of the antibacterial effects of silver nanomaterials and potential implications for human health and the environment. J Nanopart Res. 2010;12:1531–51.

    Article  CAS  Google Scholar 

  6. Chaloupka K, Malam Y, Seifalian AM. Nanosilver as a new generation of nanoproduct in biomedical applications. Tren Biotech. 2010;28:580–8.

    Article  CAS  Google Scholar 

  7. Stevens KNJ, Crespo-Biel O, van den Bosch EEM, Dias AA, Knetsch MLW, Aldenhoff YBJ, van der Veen FH, Maessen JG, Stobberingh EE, Koole LH. The relationship between the antimicrobial effect of catheter coatings containing silver nanoparticles and the coagulation of contacting blood. Biomaterials. 2009;30:3682–90.

    Article  PubMed  CAS  Google Scholar 

  8. Roe D, Karandikar B, Boon-Savage N, Gibbins B, Roullet JB. Antimicrobial surface functionalization of plastic catheters by silver nanoparticles. J Antimicrob Chemother. 2008;61:869–76.

    Article  PubMed  CAS  Google Scholar 

  9. Samuel U, Guggenbichler JP. Prevention of catheter-related infections: the potential of a new nano-silver impregnated catheter. Int J Antimicrob Agents. 2004;23S1:S75–8.

    Article  Google Scholar 

  10. Hung HS, Hsu SH. Biological performances of poly(ether)urethane-silver nanocomposites. Nanotech. 2007;18:475101.

    Article  Google Scholar 

  11. Maneerung T, Tokura S, Rujiravanit R. Impregnation of silver nanoparticles into bacterial cellulose for antimicrobial wound dressing. Carbohydrate Polym. 2008;72:43–51.

    Article  CAS  Google Scholar 

  12. Alt V, Bechert T, Steinrucke P, Wagener M, Seidel P, Dingeldein E, Domann E, Schnettler R. An in vitro assessment of the antibacterial properties and cytotoxicity of nanoparticulate silver bone cement. Biomaterials. 2004;25:4383–91.

    Article  PubMed  CAS  Google Scholar 

  13. Kassaee MZ, Akhavan A, Sheikh N, Sodagar A. Antibacterial effects of a new dental acrylic resin containing silver nanoparticles. J Appl Polym Sci. 2008;110:1699–703.

    Article  CAS  Google Scholar 

  14. Feng QL, Wu J, Chen GQ, Cui FZ, Kim TN, Kim JO. A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. J Biomed Mater Res. 2000;52:662–8.

    Article  PubMed  CAS  Google Scholar 

  15. Matsumura Y, Yoshikata K, Kunisaki S, Tsuchido T. Mode of bactericidal action of silver zeolite and its comparison with that of silver nitrate. Appl Environ Microbiol. 2003;69:4278–81.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  16. Sondi I, Salopek-Sondi B. Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. J Coll Inter Sci. 2004;275:177–82.

    Article  CAS  Google Scholar 

  17. Hwang ET, Lee JH, Chae YJ, Kim YS, Kim BC, Sang BI, Gu MB. Analysis of the toxic mode of action of silver nanoparticles using stress-specific bioluminescent bacteria. Small. 2008;4:746–50.

    Article  PubMed  CAS  Google Scholar 

  18. Stohs SJ, Bagchi D. Oxidative mechanisms in the toxicity of metal-ions. Free Radic Biol Med. 1995;18:321–36.

    Article  PubMed  CAS  Google Scholar 

  19. Damm C, Münstedt H. Kinetic aspects of the silver ion release from antimicrobial polyamide/silver nanocomposites. Appl Phys A. 2008;91:479–86.

    Article  CAS  Google Scholar 

  20. Neal AL. What can be inferred from bacterium-nanoparticle interactions about the potential consequences of environmental exposure to nanoparticles? Ecotoxicol. 2008;17:362–71.

    Article  CAS  Google Scholar 

  21. Pollini M, Paladini F, Catalono M, Taurino A, Licciulli A, Maffezzoli A, Sannino A. Antibacterial coatings on haemodialysis catheters by photochemical deposition of silver nanoparticles. J Mater Sci Mater Med. 2011;22:2005–12.

    Article  PubMed  CAS  Google Scholar 

  22. Paladini F, Pollini M, Talà A, Alifano P, Sannino A. Efficacy of silver treated catheters for haemodialysis in preventing bacterial adhesion. J Mater Sci Mater Med. 2012;23:1983–90.

    Article  PubMed  CAS  Google Scholar 

  23. AshaRani PV, Mun GLK, Hande MP, Valiyaveettil S. Cytotoxicity and genotoxicity of silver nanoparticles in human cells. ACS Nano. 2009;3:279–90.

    Article  PubMed  CAS  Google Scholar 

  24. Veenstra DL, Saint S, Sullivan SD. Cost-effectiveness of antiseptic-impregnated central venous catheters for the prevention of catheter-related bloodstream infection. JAMA. 1999;282:554–60.

    Article  PubMed  CAS  Google Scholar 

  25. Terazawa E, Shimonaka H, Nagase K, Masue T. Severe anaphylactic reaction due to a chlorhexidine-impregnated central venous catheter. Anesthesiology. 1998;89:1296–8.

    Article  PubMed  CAS  Google Scholar 

  26. Furuzono T, Yasuda S, Kimura T, Kyotani S, Tanaka J, Kishida A. Nano-scaled hydroxyapatite/polymer composite IV. Fabrication and cell adhesion of a 3D scaffold made of composite material with a silk fibroin substrate to develop a percutaneous device. J Artif Organs. 2004;7:137–44.

    Article  PubMed  CAS  Google Scholar 

  27. Furuzono T, Ueki M, Kitamura H, Oka K, Imai E. Histological reaction of sintered nano-hydroxyapatite-coated cuff and its fibroblast-like cell hybrid for an indwelling catheter. J Biomed Mater Res B Appl Biomater. 2009;89B:77–85.

    Article  CAS  Google Scholar 

  28. Midy V, Rey C, Bres E, Dard M. Basic fibroblast growth factor adsorption and release properties of calcium phosphate. J Biomed Mater Res. 1998;41:405–11.

    Article  PubMed  CAS  Google Scholar 

  29. Brust M, Walker M, Bethell D, Schiffrin DJ, Whyman R. Synthesis of thiol-derivatised gold nanoparticles in a two-phase liquid–liquid system. J Chem Soc Chem Commun. 1994; 801–802.

  30. Teranishi T, Hasegawa S, Shimizu T, Miyake M. Heat-induced size evolution of gold nanoparticles in the solid state. Adv Mater. 2011;13:1699–701.

    Article  Google Scholar 

  31. Antimicrobial products—test for antimicrobial activity and efficacy. Jpn Ind Standard JIS Z 2801; 2010.

  32. Chung C, Lee M. Self-assemble monolayers of mercaptoacetic acid on Ag powder: characterization by FT-IR diffuse reflection spectroscopy. Bull Korean Chem Soc. 2004;25:1461–2.

    Article  CAS  Google Scholar 

  33. Goto Y, Tetsumoto T. Silver nanoparticle. J Adh Soc Jpn. 2008;44:414–9 (in Japanese).

    Google Scholar 

  34. Vigneshwaran N, Nachane RP, Balasubramanya RH, Varadarajan PV. A novel one-pot ‘green’ synthesis of stable silver nanoparticles using soluble starch. Carbohydr Res. 2006;341:2012–8.

    Article  PubMed  CAS  Google Scholar 

  35. Shahverdi AR, Minaeian S, Shahverdi HR, Jamalifar H, Nohi AA. Rapid synthesis of silver nanoparticles using culture supernatants of Enterobacteria: a novel biological approach. Process Biochem. 2007;42:919–23.

    Article  CAS  Google Scholar 

  36. Chen P, Song L, Lui Y, Fang Y. Synthesis of silver nanoparticles by γ-ray irradiation in acetic water solution containing chitosan. Rad Phys Chem. 2007;76:1165–8.

    Article  CAS  Google Scholar 

  37. Xu GN, Qiao XL, Qiu XL, Chen JG. Preparation and characterization of stable monodisperse silver nanoparticles via photoreduction. Colloids Surf A Physicochem Eng Aspects. 2008;320:222–6.

    Article  CAS  Google Scholar 

  38. Tien DC, Tseng KH, Liao CY, Tsung TT. Colloidal silver fabrication using the spark discharge system and its antimicrobial effect on Staphylococcus aureus. Med Eng Phys. 2008;30:948–52.

    Article  PubMed  Google Scholar 

  39. Kora AJ, Manjusha R, Arunachalam J. Superior bactericidal activity of SDS capped silver nanoparticles: synthesis and characterization. Mater Sci Eng C. 2009;29:2104–9.

    Article  CAS  Google Scholar 

  40. Graf P, Mantion A, Foelske A, Shkilnyy A, Mašić A, Thünemann AF, Taubert A. Peptide-coated silver nanoparticles: synthesis, surface chemistry, and pH-triggered, reversible assembly into particle assemblies. Chemistry. 2009;15:5831–44.

    Article  PubMed  CAS  Google Scholar 

  41. Wang W, Chen X, Efrima S. Silver nanoparticles capped by long-chain unsaturated carboxylates. J Phys Chem B. 1999;103:7238–46.

    Article  CAS  Google Scholar 

  42. Motte L, Pileni MP. Self-assemblies of silver sulfide nanocrystals: influence of length of thio-alkyl chains used as coating agent. Appl Surf Sci. 2000;164:60–7.

    Article  CAS  Google Scholar 

  43. Li D, Hong B, Fang W, Guo Y, Lin R. Preparation of well-dispersed silver nanoparticles for oil-based nanofluids. Ind Eng Chem Res. 2010;49:1697–702.

    Article  CAS  Google Scholar 

  44. Yamamoto M, Kashiwagi Y, Nakamoto M. Size-controlled synthesis of monodispersed silver nanoparticles capped by long-chain alkyl carboxylates for silver carboxylate and tertiary amine. Langmuir. 2006;22:8581–6.

    Article  PubMed  CAS  Google Scholar 

  45. Martínez-Castañón GA, Niño-Martínez N, Martínez-Guterrez F, Martínez-Mendoza JR, Ruiz F. Synthesis and antibacterial activity of silver nanoparticles with different sizes. J Nanopart Res. 2008;10:1343–8.

    Article  Google Scholar 

Download references

Acknowledgments

A part of this work was supported by a Kinki University Research Grant, and the Feasibility Study Stage of Adaptable and Seamless Technology Transfer Program through Target-driven R&D (A-STEP), Japan Science and Technology Agency (JST), Japan. The authors thank Dr. K. Yamamoto, Graduate School of Science and Engineering, Kagoshima University, for TG/DTA measurements and useful discussion. Finally, we especially appreciate the assistance of Mr. Y. Kitafuji, Kinki University, for all experiments.

Author information

Authors and Affiliations

  1. Department of Biomaterial Engineering, School of Biology-Oriented Science and Technology, Kinki University, 930 Nishimitani, Kinokawa, Wakayama, 649-6493, Japan

    Tsutomu Furuzono & Takashi Iwamoto

  2. Department of Surgery, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita, Osaka, 565-0871, Japan

    Tsutomu Furuzono & Yoshiki Sawa

  3. Department of Science and Technology on Food Safety, School of Biology-Oriented Science and Technology, Kinki University, 930 Nishimitani, Kinokawa, Wakayama, 649-6493, Japan

    Yoshinao Azuma

  4. Department of Biomaterials, Osaka Dental University, 8-1 Kuzuha-Hanazono, Hirakata, Osaka, 573-1121, Japan

    Masahiro Okada

Authors
  1. Tsutomu Furuzono
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Takashi Iwamoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yoshinao Azuma
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Masahiro Okada
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yoshiki Sawa
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Tsutomu Furuzono or Yoshiki Sawa.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (PPTX 2988 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Furuzono, T., Iwamoto, T., Azuma, Y. et al. Preparation of carboxylated Ag nanoparticles as a coating material for medical devices and control of antibacterial activity. J Artif Organs 16, 451–457 (2013). https://doi.org/10.1007/s10047-013-0715-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10047-013-0715-3

Keywords

Search

Navigation