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Porous scaffolds of gelatin from the marine gastropod Ficus variegate with commercial cross linkers for biomedical applications

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Abstract

Gelatin was extracted from 3 (body, foot, and viscera) different body parts of Ficus variegata using an alkaline treatment. Composite scaffolds were prepared for tissue engineering applications. Proximate analysis of F. variegata prior to extraction showed percentage values of 16.67–14.33% for protein, 74.53–72.72% for moisture, 3.63–2% for ash, and 2.33–0.33% for lipids. Extracted gelatin was characterized using Fourier transform infrared (FTIR), SDS-PAGE, and amino acid analysis. Composite scaffolds using alkali treated gelatin of all 3 body parts with 4 different commercial cross-linkers [chitosan, hydroxy apatite (HA), glutaraldehyde, and nano hydroxy apatite (nHA)] were prepared and structural morphology and porosity were analyzed using scanning electron microscopy (SEM). The nHA blended gelatin scaffolds had a pore size ranging 1–10 μm. The swelling ratio, in vitro degradation, and the protein adsorption capacity was observed to be high in chitosan blended gelatin scaffolds.

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Authors and Affiliations

  1. Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, 603203, Chennai, Tamilnadu, India

    R. A. Nazeer & U. Sri Suganya

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  1. R. A. Nazeer
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  2. U. Sri Suganya
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Correspondence to R. A. Nazeer.

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Nazeer, R.A., Sri Suganya, U. Porous scaffolds of gelatin from the marine gastropod Ficus variegate with commercial cross linkers for biomedical applications. Food Sci Biotechnol 23, 327–335 (2014). https://doi.org/10.1007/s10068-014-0046-z

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  • DOI: https://doi.org/10.1007/s10068-014-0046-z

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