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Effect of laser and LED phototherapies on the healing of cutaneous wound on healthy and iron-deficient Wistar rats and their impact on fibroblastic activity during wound healing

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Abstract

Iron deficiency impairs the formation of hemoglobin, red blood cells, as well the transport of oxygen. The wound healing process involves numerous functions, many of which are dependent on the presence of oxygen. Laser has been shown to improve angiogenesis, increases blood supply, cell proliferation and function. We aimed to study the effect of λ660 nm laser and λ700 nm light-emitting diode (LED) on fibroblastic proliferation on cutaneous wounds on iron-deficient rodents. Induction of iron anemia was carried out by feeding 105 newborn rats with a special iron-free diet. A 1 × 1 cm wound was created on the dorsum of each animal that were randomly distributed into seven groups: I, control anemic; II, anemic no treatment; III, anemic + L; IV, anemic + LED; V, healthy no treatment; VI, healthy + laser; VII, healthy + LED (n = 15 each). Phototherapy was carried out using either a diode laser (λ660 nm, 40 mW, 10 J/cm2) or a prototype LED device (λ700 ± 20 nm, 15 mW, 10 J/cm2). Treatment started immediately after surgery and was repeated at 48-h interval during 7, 14, and 21 days. After animal death, specimens were taken, routinely processed, cut, stained with hematoxylin-eosin, and underwent histological analysis and fibroblast counting. Significant difference between healthy and anemic subjects on regards the number of fibroblast between treatments was seen (p < 0.008, p < 0.001). On healthy animals, significant higher count was seen when laser was used (p < 0.008). Anemic subjects irradiated with LED showed significantly higher count (p < 0.001). It is concluded that the use of LED light caused a significant positive biomodulation of fibroblastic proliferation on anemic animals and laser was more effective on increasing proliferation on non-anemics.

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Authors and Affiliations

  1. Center of Biophotonics, School of Dentistry, Federal University of Bahia, Av. Araújo Pinho, 62, Canela, Salvador, BA, 40110-150, Brazil

    Susana C. P. Oliveira Sampaio, Juliana S. de C. Monteiro, Jean Nunes dos Santos & Antonio L. B. Pinheiro

  2. Oral Epidemiology and Public Health, School of Dentistry, Federal University of Bahia, Salvador, BA, 40110-150, Brazil

    Maria Cristina T. Cangussú

  3. Laboratory of Parasitic Biomorphology, Institute Gonçalo Muniz–FIOCRUZ-BA, Salvador, BA, Brazil, 40296-710

    Gustavo M. Pires Santos & Marcos André Vannier dos Santos

  4. Laboratory of Surgical Pathology, School of Dentistry, Federal University of Bahia, Av. Araújo Pinho, 62, Canela, Salvador, BA, 40110-150, Brazil

    Jean Nunes dos Santos

  5. National Institute of Optics and Photonics, Physics Institute of São Carlos, University of São Paulo, São Carlos, SP, Brazil, 13560-970

    Jean Nunes dos Santos & Antonio L. B. Pinheiro

  6. Institute of Biomedical Engineering, Unicastelo, São José dos Campos, São José, SP, Brazil, 12245-230

    Antonio L. B. Pinheiro

Authors
  1. Susana C. P. Oliveira Sampaio
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  2. Juliana S. de C. Monteiro
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  3. Maria Cristina T. Cangussú
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  4. Gustavo M. Pires Santos
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  5. Marcos André Vannier dos Santos
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  6. Jean Nunes dos Santos
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  7. Antonio L. B. Pinheiro
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Correspondence to Antonio L. B. Pinheiro.

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Oliveira Sampaio, S.C.P., de C. Monteiro, J.S., Cangussú, M.C.T. et al. Effect of laser and LED phototherapies on the healing of cutaneous wound on healthy and iron-deficient Wistar rats and their impact on fibroblastic activity during wound healing. Lasers Med Sci 28, 799–806 (2013). https://doi.org/10.1007/s10103-012-1161-9

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  • DOI: https://doi.org/10.1007/s10103-012-1161-9

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