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Effects of red and near-infrared LED light therapy on full-thickness skin graft in rats

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Abstract

The aim of the present study was to evaluate the in vivo response of different wavelengths (red and near-infrared) of light-emitting diode (LED) on full-thickness skin grafts (FTSG) in rats. Thirty rats were randomly allocated into three experimental groups: control group (C); red LED treated group (R); and near-infrared LED group (NIR). Skin grafts were irradiated daily for ten consecutive days, starting immediately after the surgery using a red (630 nm) or near-infrared (850 nm) LED. The results showed that the red wavelength LED significantly enhanced the skin graft score in relation to the NIR group and increased transforming growth factor beta (TGF-β) protein expression and density of collagen fibers compared with the other experimental groups. These results suggest that the red wavelength LED was efficient to improve the dermo-epidermal junction and modulate the expression proteins related to tissue repair.

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Funding

This study was financially supported by the Brazilian funding agency FAPESP project # 2015/13501-3.

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Author notes

  1. Cintia Cristina Santi Martignago and Carla Roberta Tim contributed equally to this work.

Authors and Affiliations

  1. Department of Physiotherapy, Federal University of São Carlos, São Carlos, SP, Brazil

    Cintia Cristina Santi Martignago, Viviane Ribeiro Da Silva, Estefany Camila Bonfim Dos Santos, Fabiana Nascimento Vieira, Nivaldo Antonio Parizotto & Richard Eloin Liebano

  2. Scientific Institute and Technological Department-University Brazil, São Paulo-Itaquera, SP, Brazil

    Carla Roberta Tim, Lívia Assis & Nivaldo Antonio Parizotto

  3. Federal University of São Paulo, UNIFESP, Santos, SP, Brazil

    Lívia Assis

  4. Biotechnology in Regenerative Medicine and Medical Chemistry, University of Araraquara, Araraquara, SP, Brazil

    Nivaldo Antonio Parizotto

Authors
  1. Cintia Cristina Santi Martignago
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  2. Carla Roberta Tim
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  3. Lívia Assis
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Correspondence to Cintia Cristina Santi Martignago.

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The present study was approved by the Animal Care Committee guidelines at the Federal University of São Carlos (protocol 8577280716) and it was conducted according to the Guiding Principles for the Use of Laboratory Animals.

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Martignago, C.C.S., Tim, C.R., Assis, L. et al. Effects of red and near-infrared LED light therapy on full-thickness skin graft in rats. Lasers Med Sci 35, 157–164 (2020). https://doi.org/10.1007/s10103-019-02812-6

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