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Improvement in mechanical properties and biocompatibility of biosynthetic bacterial cellulose/lotus root starch composites

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Abstract

Bacterial cellulose/lotus root starch (BC/LRS) composites were prepared by cultivating Acetobacter xylinum in nutrient media containing gelatinized lotus root starch. Low concentrations of gelatinized LRS had increased BC production with the maximum value at 6.67 g/L when 5 g/L of LRS was added in the culture media and the composites had thicker and denser fibrils compared with those of BC with low concentrations of LRS (2.5 and 5 g/L). When the concentration of LRS was increased above 7.5 g/L, the morphology of the BC/LRS composites contained more fibril layers that were linked with LRS. The results from X-ray diffraction (XRD) demonstrated that there was no significant difference in structure between BC and BC/LRS composites except a slight increase in crystallinity for BC/LRS composites as the concentration of LRS was lifted up. The tensile tests were performed to display BC/LRS composites prepared with LRS concentration at 2.5 and 5 g/L in media had the tensile strength of 54 and 60 MPa, respectively, which indicated an improvement in mechanical property compared to the unmodified BC (45 MPa). Live/dead assay with chondrocytes seeded on BC/LRS composite revealed higher cell viability ranging from 85% to 90% than BC. Furthermore, cell morphology with typical spindle shape was observed on the surfaces of BC/LRS composite by confocal microscope. Through the overall results, it shows that this study has provided a guidance to prepare BC/LRS composites with better cell biocompatibility and higher mechanical strength than those of BC for the potential use in cartilage tissue engineering.

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Authors and Affiliations

  1. State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai, 201620, China

    Na Yin, Shi-yan Chen  (陈仕艳) & Hua-ping Wang

  2. Department of Obstetrics and Gynecology, Affiliated Sixth People’s Hospital, Shanghai Jiaotong University, Shanghai, 200233, China

    Yi-meng Cao & Qing-kai Wu  (吴氢凯)

Authors
  1. Na Yin
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  2. Shi-yan Chen  (陈仕艳)
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  3. Yi-meng Cao
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  4. Hua-ping Wang
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  5. Qing-kai Wu  (吴氢凯)
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Corresponding authors

Correspondence to Shi-yan Chen  (陈仕艳) or Qing-kai Wu  (吴氢凯).

Additional information

This work was financially supported by the National Natural Science Foundation of China (Nos. 51273043, 51573024 and 81550008) and the Fundamental Research Funds for the Central Universities and DHU Distinguished Young Professor Program.

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Yin, N., Chen, Sy., Cao, Ym. et al. Improvement in mechanical properties and biocompatibility of biosynthetic bacterial cellulose/lotus root starch composites. Chin J Polym Sci 35, 354–364 (2017). https://doi.org/10.1007/s10118-017-1903-z

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  • DOI: https://doi.org/10.1007/s10118-017-1903-z

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