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Establishment of a Genetic Transformation System for the Marine Pennate Diatom Fistulifera sp. Strain JPCC DA0580—A High Triglyceride Producer

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Abstract

A genetic transformation system for the marine pennate diatom, Fistulifera sp. JPCC DA0580, was established using microparticle bombardment methods. Strain JPCC DA0580 has been recently identified as the highest triglyceride (60 % w/w) producer from a culture collection of 1,393 strains of marine microalgae, and it is expected to be a feasible source of biodiesel fuel. The transformation conditions for strain JPCC DA0580 were optimised using the green fluorescent protein gene (gfp) and the gene encoding neomycin phosphotransferase II (nptII). The most efficient rate of transformation was attained when tungsten particles (0.6 μm in diameter) were used for microparticle bombardment. The effect of endogenous and exogenous promoters on the expression of nptII was examined. Endogenous promoters were more efficient for obtaining transformants compared with exogenous promoters. Southern hybridisation analysis suggested that nptII integrated into the nuclear genome. This genetic manipulation technique should allow us to understand the mechanisms of high triglyceride accumulation in this strain, thereby contributing to improving BDF production.

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Acknowledgments

This work was supported by JST, CREST.

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Authors and Affiliations

  1. Graduate School of Bio-Applications and Systems Engineering, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo, 184-8588, Japan

    Masaki Muto & Yorikane Fukuda

  2. Cooperative Major in Advanced Health Science, Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo, 184-8588, Japan

    Michiko Nemoto

  3. Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo, 184-8588, Japan

    Tomoko Yoshino, Tadashi Matsunaga & Tsuyoshi Tanaka

  4. JST, CREST, Sanbancho 5, Chiyoda-ku, Tokyo, 102-0075, Japan

    Masaki Muto & Tsuyoshi Tanaka

Authors
  1. Masaki Muto
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  2. Yorikane Fukuda
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  3. Michiko Nemoto
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  4. Tomoko Yoshino
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  5. Tadashi Matsunaga
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  6. Tsuyoshi Tanaka
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Correspondence to Tsuyoshi Tanaka.

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Muto, M., Fukuda, Y., Nemoto, M. et al. Establishment of a Genetic Transformation System for the Marine Pennate Diatom Fistulifera sp. Strain JPCC DA0580—A High Triglyceride Producer. Mar Biotechnol 15, 48–55 (2013). https://doi.org/10.1007/s10126-012-9457-0

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  • DOI: https://doi.org/10.1007/s10126-012-9457-0

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