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Genetic variation among different populations of Aster tripolium grown on naturally and anthropogenic salt-contaminated habitats: implications for conservation strategies

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Abstract

The sea aster, Aster tripolium L., grows naturally in temperate regions, mainly in the salt meadows close to the coast. The species is also found in naturally and anthropogenically salt-contaminated inland habitats, such as potash mine dumps. The genetic relationships among populations from different habitats and correlations of the genotype with physiological and vegetational parameters were investigated. A. tripolium plants from five different sites close to the seashore on the North Sea island Baltrum, from five different potash mine dumps and, as an outgroup, from the seashore in Japan were probed. DNA was extracted from five plants from each of the 11 A. tripolium populations and analyzed for random amplified polymorphic DNA (RAPD). Altogether 35 polymorphic bands in 51 individuals and 45 different detectable genotypes could be identified. For evaluation of the genetic variation using RAPD bands, the neighbor-joining method, the principal coordinate analysis, and the analysis of molecular variance were applied, resulting in the classification into three genetic groups. A. tripolium plants from different ecological habitats on Baltrum were closely related while the plants growing at the deposit dumps showed a higher genetic diversity. The Japanese population was genetically very different from the German populations. Correlations between phytosociological and soil parameters and the respective genotype were not significant. The results argue for a conservation of anthropogenically salt-contaminated habitats to maintain genetic variability not only on the species level, but also within a species.

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Abbreviations

AMOVA:

analysis of molecular variance

EDXA:

energy dispersive X-ray-microanalysis

FES:

flame emission spectrometry

PCoA:

principal coordinates analysis

RAPD:

random amplified polymorphic DNA

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Acknowledgments

We appreciate the valuable work of Yvonne Leye during outdoor sample collecting and cultivating A. tripolium in the greenhouse. We would like to thank Hermann Wietjes, Baltrum, for his help during measuring the sample site on Baltrum. The seeds of Aster tripolium collected in Japan were gratefully obtained from Miwiga Takeda and Dr. Yuichi Uno, Kobe, Japan. We would like to thank Aminul Islam, Hannover, for initial help with the RAPD analysis and constant interest.

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Authors and Affiliations

  1. Institut für Botanik, Universität Hannover, Herrenhäuserstr. 2, 30419, Hannover, Germany

    Jörg Brock, Sabine Aboling & Jutta Papenbrock

  2. Institut für Tierökologie und Zellbiologie, Tierärztliche Hochschule, Bünteweg 17d, 30559, Hannover, Germany

    Ralf Stelzer

  3. Institut für Angewandte Genetik, Universität Hannover, Herrenhäuserstr. 2, 30419, Hannover, Germany

    Elisabeth Esch

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  1. Jörg Brock
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  2. Sabine Aboling
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  3. Ralf Stelzer
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  4. Elisabeth Esch
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  5. Jutta Papenbrock
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Correspondence to Jutta Papenbrock.

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Brock, J., Aboling, S., Stelzer, R. et al. Genetic variation among different populations of Aster tripolium grown on naturally and anthropogenic salt-contaminated habitats: implications for conservation strategies. J Plant Res 120, 99–112 (2007). https://doi.org/10.1007/s10265-006-0030-7

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  • DOI: https://doi.org/10.1007/s10265-006-0030-7

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