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Environmentally friendly bleaching of cotton using laccases

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Abstract

A new strain of Trametes hirsuta was found to oxidize various cotton flavonoids. Here we show that laccases of this organism were responsible for oxidation of the flavonoids morin, luteolin, rutin and quercetin. Out of two laccases produced by T. hirsuta (60.7 and 51.0 kDa) the more prominent 60.7 kDa laccase was purified and showed K m and k cat values of 75.5, 20.9 and 49.4 μM and 72.5, 96.3 and 32.7 s−1, hours on ABTS, syringaldazide and DMP, respectively. Pretreatment of cotton with the T. hirsuta laccase resulted in a whiteness increase of 8.5%.

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References

  • Abadulla E, Tzanov T, Costa S, Robra KH, Cavaco-Paulo A, Gübitz GM (2000) Decolorisation and detoxification of textile dyes with a laccase from Trametes hirsuta. Appl Environ Microbiol 66:3357–3362

    Article  Google Scholar 

  • Almansa E, Kandelbauer A, Pereira L, Cavaco-Paulo A, Gübitz GM (2004) Influence of structure on dye degradation with laccase mediator systems. Biocatal Biotransform 22:315–324

    Article  Google Scholar 

  • Ardon O, Kerem Z, Hadar Y (1996) Enhancement of laccase activity in liquid cultures of the ligninolytic fungus Pleurotus ostreatus by cotton stalk extract. J Biotechnol 51:201–207

    Article  Google Scholar 

  • Becard G, Douds DD, Pfeffer P (1992) Extensive in vitro hyphal growth of vesicular–arbuscular mycorrhizal fungi in the presence of carbon dioxide and flavonols. Appl Environ Microbiol 58:821–825

    Google Scholar 

  • Hedin PA, Jenkis JN, Parrot WL (1992) Evaluation of flavonoids in Gossypium arboretum (L.) cottons as potential source of resistance to Tobacco, Budworm. J Chem Ecol 18

  • Kandelbauer A, Guebitz GM (2005) Bioremediation for the decolorisation of textile dyes, a review. In: Lichtfouse E, Schwarzbauer J, Robert D (eds) Environmental chemistry. Springer-Verlag, Heidelberg, pp 269–288

    Chapter  Google Scholar 

  • Kurisawa M, Chung Joo E, Uyama H, Kobayashi S (2003) Enzymatic synthesis and antioxidant properties of poly(rutin). Biomacromolecules 4:1394–1399

    Article  PubMed  Google Scholar 

  • Makris DP, Rossiter JT (2002) An investigation on structural aspects influencing product formation in enzymic and chemical oxidation of quercetin and related flavonols. Food Chem 77:177–185

    Article  Google Scholar 

  • Mejias L, Reihmann MH, Sepulveda-Boza S, Ritter H (2002) New polymers from natural phenols using horseradish or soybean peroxidase. Macromol Biosci 21:24–32

    Article  Google Scholar 

  • Pickard MA, Westlake DWS (1970) Fungal metabolism of flavonoids. Purification, properties, and substrate specificity of an inducible laccase from Polyporus versicolor PRL 572. Can J Microbiol 48:1351–1358

    Google Scholar 

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Acknowledgements

This study was supported by the European Project OXITEX

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Authors and Affiliations

  1. Department of Environmental Biotechnology, Graz University of Technology, Petersgasse 12, 8010, Graz, Austria

    L. Pereira & G. M. Guebitz

  2. Department of Textile Engineering, University of Minho, 4800, Guimarães, Portugal

    C. Bastos, T. Tzanov & A. Cavaco-Paulo

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  1. L. Pereira
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  2. C. Bastos
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  3. T. Tzanov
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  4. A. Cavaco-Paulo
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  5. G. M. Guebitz
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Corresponding author

Correspondence to G. M. Guebitz.

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Pereira, L., Bastos, C., Tzanov, T. et al. Environmentally friendly bleaching of cotton using laccases. Environ Chem Lett 3, 66–69 (2005). https://doi.org/10.1007/s10311-005-0004-3

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  • DOI: https://doi.org/10.1007/s10311-005-0004-3

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