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Investigation of bioremediation potential of zymogenous bacteria and fungi for crude oil degradation

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Abstract

Bioremediation potential of bacteria and fungi isolated from sludge samples has been investigated (Danube alluvium, Pančevo, Serbia). Total isolated microorganisms were divided into three parts. One part was added with actidione antifungicide. The second part was added with streptomycin antibiotic. The third part was without additives. Paraffinic type of crude oil was a substrate for assessment of bioremediation potential. The simulated oil biodegradation lasted 30, 60 and 90 days. Parallel with that, the experiments with blind trial were conducted. Extracts were isolated from the samples with chloroform in a separate funnel. They were assayed for the group composition (alkanes, aromatics, alcohols and fatty acids) by column chromatography. Alkane fraction was analysed by gas chromatography—mass spectrometry (GC–MS). The most intense oil degradation was achieved in the experiments with bacteria, somewhat weaker with consortium of fungi and bacteria, and the weakest bioremediation potential in these experiments was shown by fungi.

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Acknowledgments

We thank the Alexander von Humboldt Foundation and the Ministry of Science and Technological Development of the Republic of Serbia (Projects: 146008 & 142018) for supporting this research.

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Authors and Affiliations

  1. Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11001, Belgrade, Serbia

    Miroslav Vrvić & Branimir Jovančićević

  2. Center of Chemistry, IChTM, Studentski trg 12-16, 11001, Belgrade, Serbia

    Mila Ilić, Mališa Antić, Vesna Antić, Miroslav Vrvić & Branimir Jovančićević

  3. Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11081, Belgrade, Serbia

    Mališa Antić

  4. Institute of Geology and Geochemistry of Petroleum and Coal, Aachen University, Lochenerstrasse 4-20, 52056, Aachen, Germany

    Jan Schwarzbauer

Authors
  1. Mila Ilić
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  2. Mališa Antić
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  3. Vesna Antić
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  4. Jan Schwarzbauer
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  5. Miroslav Vrvić
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  6. Branimir Jovančićević
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Correspondence to Branimir Jovančićević.

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Ilić, M., Antić, M., Antić, V. et al. Investigation of bioremediation potential of zymogenous bacteria and fungi for crude oil degradation. Environ Chem Lett 9, 133–140 (2011). https://doi.org/10.1007/s10311-009-0257-3

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  • DOI: https://doi.org/10.1007/s10311-009-0257-3

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