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Determination of the Molar-Mass Averages of Random Poly(aspartate-co-lactide) Copolymers by Tuning the Ionic Strength of the Solvent

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Abstract

Water-soluble sodium poly(aspartate-co-lactide) (PALNa) copolymers with a molar ratio of aspartate-to-lactide units equal to 1:0.6, 1:1.0 and 1:1.5 were studied using NMR spectroscopy to determine the composition as well as SEC-MALS and static light-scattering measurements to determine the molar-mass characteristics of the copolymers. In the copolymer aqueous solutions, high-molar-mass species were detected, most probably due to the incomplete dissolution of the samples. The molar-mass averages determined in water with added simple electrolyte, i.e., NaCl, were much lower than the values determined in pure water. The concentration of the salt, which allows dissolution on a molecular level, and the separation predominantly according to a size-exclusion mechanism depend on the chemical composition of the PALNa copolymers. The optimal mobile phase for the PALNa-1/0.6 and the PALNa-1/1.0 copolymers was 0.1 M NaCl at pH 9, and for the PALNa-1/1.5 copolymer with a higher content of lactide units it was 0.05 M NaCl at pH 9. The molar-mass averages of the PALNa-1/1.0 copolymer, determined by SEC-MALS and static light-scattering measurements, were comparable.

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Acknowledgments

The authors gratefully acknowledge the financial support of the Ministry of Higher Education, Science and Technology of the Republic of Slovenia and the Slovenian Research Agency (program P2-0145 and Ph.D. fellowship 3311-04-831033). This work was supported by the EU project Nanobiopharmaceuticals (NMP4-CT-20006-026723). The authors also wish to thank Dr. Andrej Kržan and Ms. Maja Kerovec for contribution to the synthesis of the poly(aspartate-co-lactide) copolymers.

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Authors and Affiliations

  1. Laboratory for Polymer Chemistry and Technology, National Institute of Chemistry, Hajdrihova 19, 1000, Ljubljana, Slovenia

    Maja Gričar, Majda Žigon, Tina Šmigovec Ljubič & Ema Žagar

  2. Center of Excellence for Polymer Materials and Technologies, Tehnološki park 24, 1000, Ljubljana, Slovenia

    Majda Žigon & Ema Žagar

  3. EN-FIST Centre of Excellence, Dunajska 156, 1000, Ljubljana, Slovenia

    Ema Žagar

  4. University College for Polymer Technology, Glavni trg 1, 2380, Slovenj Gradec, Slovenia

    Majda Žigon

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  1. Maja Gričar
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  2. Majda Žigon
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  3. Tina Šmigovec Ljubič
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  4. Ema Žagar
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Correspondence to Ema Žagar.

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Gričar, M., Žigon, M., Ljubič, T.Š. et al. Determination of the Molar-Mass Averages of Random Poly(aspartate-co-lactide) Copolymers by Tuning the Ionic Strength of the Solvent. Chromatographia 75, 205–212 (2012). https://doi.org/10.1007/s10337-012-2180-1

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  • DOI: https://doi.org/10.1007/s10337-012-2180-1

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