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A New Stationary Phase for Hydrophilic Interaction Chromatography: Polyacrylate-Based Hydrophilic, Monosized-Porous Beads with Zwitterionic Molecular Brushes

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Abstract

Hydrophilic, polyacrylate-based, monosized-porous beads with zwitterionic molecular brushes were synthesized as a new stationary medium for hydrophilic interaction chromatography. Monosized-porous poly(glycerol-1,3-diglycerolate diacrylate-co-glycerol dimethacrylate), poly(GDGDA-co-GDMA), beads 5 μm in size were obtained by a staged-shape template polymerization. As an initiator for surface-initiated atom transfer radical polymerization (SI-ATRP), bromine functionality was obtained on the beads by reacting their hydroxyl groups with 3-(aminopropyl)triethoxysilane and α-bromoisobutyryl bromide, respectively. Zwitterionic molecular brushes on the hydrophilic poly(GDGDA-co-GDMA) beads were generated by SI-ATRP of a sulfobetaine monomer, [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl) ammonium hydroxide (MESH). Poly(MESH)-grafted poly(GDGDA-co-GDMA), poly(MESH)g-poly(GDGDAco-GDMA), beads were slurry packed into the microbore columns with 2 mm i.d. and evaluated as stationary medium for the separation of organic acids, nucleosides and peptides using microbore columns in hydrophilic interaction chromatography with the plate numbers up to 30,000 plates m−1.

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Authors and Affiliations

  1. Chemical Engineering Department, Hacettepe University, Ankara, Turkey

    Bekir Çelebi, Begüm Özen, Çiğdem Kip & Ali Tuncel

  2. Division of Nanotechnology and Nanomedicine, Hacettepe University, Ankara, Turkey

    Ali Tuncel

Authors
  1. Bekir Çelebi
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  2. Begüm Özen
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  3. Çiğdem Kip
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  4. Ali Tuncel
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Correspondence to Ali Tuncel.

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Çelebi, B., Özen, B., Kip, Ç. et al. A New Stationary Phase for Hydrophilic Interaction Chromatography: Polyacrylate-Based Hydrophilic, Monosized-Porous Beads with Zwitterionic Molecular Brushes. Chromatographia 77, 1511–1520 (2014). https://doi.org/10.1007/s10337-014-2754-1

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  • DOI: https://doi.org/10.1007/s10337-014-2754-1

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