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Laboratory evaluation of the effectiveness of the entomopathogen; Isaria farinosa, on citrus mealybug, Planococcus citri

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Abstract

Citrus mealybug Planococcus citri (Risso) (Hemiptera: Pseudococcidae) is one of the main pests on citrus trees. Biological control of the pest is based on the release of hymenopterous parasitoids and coccinellid predators at present. The effectiveness of entomopathogen fungus Isaria farinosa (Holmsk.) Fries ([Sordariomycetes: Hypocreales] (Syn: Paecilomyces farinosus), as an alternative biological control agent on citrus mealybug, was investigated using four different inoculum densities and different relative humidities (RH). The entomopathogen caused 89.39% mortality in ovisacs, 84.07% mortality in second larval stage, 84.53% mortality in adult females, and 78.71% mortality in first larval stage at 95% RH and at 1 × 108 conidia ml−1 inoculum concentration. Percent mortalities were between 50 and 60 in ovisac, first and second larval stage at 95% RH and at 1 × 107 conidia ml−1. Percent mortality was decreased parallel to the decrease in humidity level and inoculum densities; however, the fungus caused significant infection in 70% and 80% RH. These results demonstrated that the effectiveness of the entomopathogen is promising for biocontrol of citrus mealybug.

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References

  • Ali S, Huang Z, Ren S (2010) Production of cuticle degrading enzymes by Isaria fumosorosea and their evaluation as a biocontrol agent against diamondback moth. J Pest Sci 83:361–370

    Article  Google Scholar 

  • Ben-Dov YA (1994) Systematic catalogue of the mealybugs of the world (Insecta: Homoptera: Coccoidea: Pseudococcidae and Putoidae) with data on geographical distribution, host plants, biology and economic importance. Intercept Limited, Andover

  • Blumberg D, Van Driesche RG (2001) Encapsulation rates of three encyrtid parasitoids by three mealybug species (Homoptera: Pseudococcidae) found commonly as pests in commercial greenhouses. Biol Control 22:191–199

    Article  Google Scholar 

  • Bouamama N, Vidal C, Fargues J (2010) Effects of fluctuating moisture and temperature regimes on the persistence of quiescent conidia of Isaria fumosorosea. J Invert Pathol 105:139–144

    Article  CAS  Google Scholar 

  • Cabaleiro C, Segura A (1997) Some characteristics of the transmission of grapevine leafroll associated virus 3 by Planococcus citri Risso. Eur J Plant Pathol 103:373–378

    Article  CAS  Google Scholar 

  • Cantu G, Mata Pineda A, Fu Castillo, Torre de la M (2004) Control microbiano in vitro del piojo harinoso (Planococcus ficus) con Beauveria bassiana, Metarhizium anisopliae, Paecilomyces fumosoroseus y Verticillium lecanii [Microbial control of vine mealybug (Planococcus ficus) by Beauveria bassiana, Metarhizium anisopliae, Paecilomyces fumosoroseus and Verticillium lecanii] Memorias del XXVII Congreso Nacional de Control Biologico, Sociedad Mexicana de Control Biologico, Los Mochis, Sin., Mexico1113 Nov. P.395399. CIAD, Mexico

  • Castineiras A, Pena JE, Duncan R, Osborne L (1996) Potential of Beauveria bassiana and Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes) as biological control agents of Thirips palmi (Thysanoptera:Thripidae). Fla Entomol 79(3):458–461

    Article  Google Scholar 

  • De Barro PJ (1990) Natural enemies and other species associated with Saccharicoccus sacchari (Cockherell) (Hemiptera: Pseudococcidae) in the Bundaberg area, Southeast Queensland. J Aust Entomol Soc 29:87–88

    Article  Google Scholar 

  • Delalibera IJ, Humber RA, Bento JMS, De Matos AP (1997) First record of entomopathogenic fungus Neozygites fumosa on the cassava mealybug Phenacoccus herreni. J Invert Pathol 69:276–278

    Article  Google Scholar 

  • Domsch KH, Gams W, Anderson TH (1980) Compendium of soil fungi. Academic Press, London

    Google Scholar 

  • Dragonova S, Takov D, Doychev D (2007) Bioassays with isolates of Beauveria bassiana (bals.)Vuill. and Paecilomyces farinosus (holm.) Brown & Smith against Ips sexdentatus Boerner and Ips acuminatus Gyll. (Coleoptera: Scolytidae). Plant Sci 44:24–28

    Google Scholar 

  • Evans HC, Hywel-Jones NL (1997) Enthomopathogenic fungi. In: Ben Dov Y, Hudgson CJ (eds) Soft scale insect, their biology natural enemies and control, vol 7B. Elsevier, Amsterdam

    Google Scholar 

  • Evans HC, Prior C (1990) Entomopathogenic fungi. In: Rosen D (ed) Armoured scale insects, their biology, natural enemies and control. World Crop Pests, vol 4B. Elsevier, Amsterdam

    Google Scholar 

  • Flaherty DL, Peacock WL, Bettiga L, Leavitt GM (1982) Chemicals losing effect against grape mealybug. Calif Agric 36:15–16

    CAS  Google Scholar 

  • Geiger CA, Daane KM (2001) Seasonal movement and sampling of the grape mealybug, Planococcus maritimus (Ehrhorn) (Homoptera: Pseudococcidae) in San Joaquin Valley vineyards. J Econ Entomol 94:291–301

    Article  PubMed  CAS  Google Scholar 

  • Godfrey KE, Daane KM, Bentley WJ, Gill RJ, Malakar Kuenen R (2002) Mealybugs in California vineyards (Publication 21612). University of California, Agriculture & Natural Resources, Oakland

  • Gökçe A, Er MK (2005) Pathogenicity of Paecilomyces spp. to glasshouse Whitefly, Trialeurodes vaporariorum, with some observations on the fungal infection process. Turk J Agric For 29:331–339

    Google Scholar 

  • Hoddle SM (1999) The biology and management of silverleaf whitefly, Bemisia argentifolii Bellows and Perring (Homoptera: Aleyrodidae) on greenhouse grown ornamentals. http://www.biocontrol.ucr.edu/bemisia.html

  • Lacey LA, Mesquita ALM, Mercadier G, Debire R, Kazmer DJ, Leclant F (1977) Acute and subletal activity of the entomopathogenic fungus Paecilomyces fumosoroseus Deuteromycotina: Hyphomycetes) on adult Aphelinus asychis (Hymenoptera: Aphelinidae). Environ Entomol 26(6):1452–1460

    Google Scholar 

  • Le Rü B (1986) E′ tude de l’evolution d’une mycose a Neozygites fumosa (Zygomycetes, Entomophthorales) dans une population de la cochenille du manioc, Phenacoccus manihoti (Hom.: Pseudococcidae) [Epizoology of the fungus Neozygites fumosa (Zygomycetes; Entomophthorales) in a population of cassava mealybug, Phenacoccus manihoti (Hom: Pseudococcidae)]. Entomophaga 31:79–89

    Article  Google Scholar 

  • Miguel C, Pereira S, Cabaleiro C, Faoro F, Segura A (2007) Presence of Grapevine leafroll associated virus 3 in primary salivary glands of the mealybug vector Planococcus citri suggests a circulative transmission mechanism. Eur J Plant Pathol 118(1):23–30

    Article  Google Scholar 

  • Panyasiri C, Attathom T, Poehling HM (2007) Pathogenicity of entomopathogenic fungi-potential candidates to control insect pests on tomato under protected cultivation in Thailand. J Plant Dis Protect 114(6):278–287

    Google Scholar 

  • Parker BL, Skinner M, Costa SD, Gouli S, Reid W, Bouhssini M (2003) Entomopathogenic fungi of Eurygaster integriceps Puton (Hemiptera: Scutelleridae): collection and characterization for development. Biol Control 27:260–272

    Article  Google Scholar 

  • Ramoska WA (1984) The influence of relative humidity on Beauveria bassiana infectivity and replication in the chinch bug, Blissus leucopterus. J Invert Pathol 43:389–394

    Article  Google Scholar 

  • Roberts DW, Yendol GW (1971) Use of fungi for microbial control of insects. In: Burgess HD, Hussey NW (eds) Microbial control of insects and mites. Academic Press, London

    Google Scholar 

  • Samson RA (1974) Paecilomyces and some allied Hyphomycetes. Studies in Mycology, vol 6. Centraalbureau voor Schimmelcultures, Baarn

  • Sapieha-Waszkiewicz A, Marjanska Cichon B, Mietkiewski R (2004) The effect of fungicides applied in apple orchards on entomopathogenic fungi in vitro. Electron J Polish Agric Univ 7(1). http://www.ejpau.media.pl

  • Speare AT (1922) Natural control of the citrus mealybug in Florida. U. S. Dept Agric Bull 1117:1–9

    Google Scholar 

  • Tanada Y, Kaya HK (1993) Insect pathology. Academic Press Inc., San Diego

    Google Scholar 

  • Vidal C, Fargues J, Lacey LA (1997) Intraspecific variability of Paecilomyces fumosoroseus: effect of temperature on vegetative growth. J Invert Pathol 70:18–26

    Article  Google Scholar 

  • Walton VM, Pringle KL (1999) Effects of pesticides used on the table grapes on the mealybug parasitoid Coccidoxenioides perigrinus (Timberlake) (Hymenoptera: Encrytidae). S Afr J Enol Vitic 20:31–34

    CAS  Google Scholar 

  • Yang S, Zhuang H, Li Y, Kuang R (2009) Insecticidal effcacy of Isaria farinosa in different life stages of Pissodes punctatus (Coleoptera: Curculionidae). J Pest Sci 82:321–325

    Article  Google Scholar 

  • Zimmermann G (2008) The entomopathogenic fungi Isaria farinosa (formerly Paecilomyces farinosus) and the Isaria fumosorosea species complex (formerly Paecilomyces fumosoroseus): biology, ecology and use in biological control. Biocontrol Sci Technol 18(9):865–901

    Article  Google Scholar 

  • Zimmermann G (2010) Isaria farinosa, CMI Descriptions of Pathogenic Fungi and Bacteria No: 614

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Acknowledgments

This research was supported by TUBİTAK (The Scientific and Technological Research Council of Turkey) (Project no: 104 O 200). The authors are grateful to Dr. Richard Humber (USDA-ARS Biological Integrated Pest Management Unit) for his kind help in the identification of I. farinosa.

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Authors and Affiliations

  1. Faculty of Agriculture, Plant Protection Department, University of Ankara, 06110, Ankara, Turkey

    Fikret Demirci, Murat Muştu & Selma Ülgentürk

  2. Faculty of Agriculture, Plant Protection Department, Yüzüncü Yıl University, 65080, Van, Turkey

    M. Bora Kaydan

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  1. Fikret Demirci
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  2. Murat Muştu
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  3. M. Bora Kaydan
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  4. Selma Ülgentürk
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Correspondence to Fikret Demirci.

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Communicated by E. Roditakis.

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Demirci, F., Muştu, M., Bora Kaydan, M. et al. Laboratory evaluation of the effectiveness of the entomopathogen; Isaria farinosa, on citrus mealybug, Planococcus citri . J Pest Sci 84, 337–342 (2011). https://doi.org/10.1007/s10340-011-0350-9

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