Abstract
It has shown that altering crosslink density of biopolymers will regulate the morphology of Mesenchymal Stem Cells (MSCs) and the subsequent MSCs differentiation. These observations have been found in a wide range of biopolymers. However, a recent work published in Nature Materials has revealed that MSCs morphology and differentiation was unaffected by crosslink density of polydimethylsiloxane (PDMS), which remains elusive. To understand such unusual behaviour, we use nanoindentation tests and modelling to characterize viscoelastic properties and surface adhesion of PDMS with different base:crosslink ratio varied from 50:1 (50D) to 10:1 (10D). It has shown that lower crosslink density leads to lower elastic moduli. Despite lower nanoindentation elastic moduli, PDMS with lowest crosslink density has higher local surface adhesion which would affect cell-biomaterials interactions. This work suggests that surface adhesion is likely another important physical cue to regulate cell-biomaterials interactions.
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Lim, K.S., Alves, M.H., Poole-Warren, L.A., et al.: Covalent incorporation of non-chemically modified gelatin into degradable PVA-tyramine hydrogels. Biomaterials 34, 7097–7105 (2013)
Cui, H., Shao, J., Wang, Y., et al.: PLA-PEG-PLA and its electroactive tetraaniline copolymer as multi-interactive injectable hydrogels for tissue engineering. Biomacromolecules 14, 1904–1912 (2013)
Ouasti, S., Donno, R., Cellesi, F., et al.: Network connectivity, mechanical properties and cell adhesion for hyaluronic acid/PEG hydrogels. Biomaterials 32, 6456–6470 (2011)
Rao, Z., Sasaki, M., Taguchi, T.: Development of amphiphilic, enzymatically-degradable PEG-peptide conjugate as cell crosslinker for spheroid formation. Colloids and Surfaces B-Biointerfaces 101, 223–227 (2013)
Schmitt, S.K., Murphy, W.L., Gopalan, P.: Crosslinked PEG mats for peptide immobilization and stem cell adhesion. Journal of Materials Chemistry B 1, 1349–1360 (2013)
Zustiak, S.P., Wei, Y., Leach, J.B.: Protein-hydrogel interactions in tissue engineering: mechanisms and applications. Tissue Engineering Part B-Reviews 19, 160–171 (2013)
Campbell, J.J., Bader, D.L., Lee, D.A.: Mechanical loading modulates intracellular calcium signaling in human mesenchymal stem cells. Journal of Applied Biomaterials & Biomechanics 6, 9–15 (2008)
Heywood, H.K., Sembi, P.K., Lee, D.A., et al.: Cellular utilization determines viability and matrix distribution profiles in chondrocyte-seeded alginate constructs. Tissue Engineering 10, 1467–1479 (2004)
Chen, J., Irianto, J., Inamdar, S., et al.: Cell mechanics, structure, and function are regulated by the stiffness of the three-dimensional microenvironment. Biophysical Journal 103, 1188–1197 (2012)
Chen, J., Bader, D.L., Lee, D.A., et al.: Finite element modeling of cell deformation when chondrocyte seeded agarose is subjected to compression. In: Elhaj A., Bader D. eds., Proc. of 8th International Conference on Cell & Stem Cell Engineering, 17–20 (2011)
Engler, A.J., Sen, S., Sweeney, H.L., et al.: Matrix elasticity directs stem cell lineage specification. Cell 126, 677–689 (2006)
Lee, J., Abdeen, A.A., Zhang, D., et al.: Directing stem cell fate on hydrogel substrates by controlling cell geometry, matrix mechanics and adhesion ligand composition. Biomaterials 34, 8140–8148 (2013)
Trappmann, B., Gautrot, J.E., Connelly, J.T., et al.: Extracellular-matrix tethering regulates stem-cell fate. Nature Materials 11, 642–649 (2012)
Panou, A.I., Papadokostaki, K.G., Tarantili, P.A., et al.: Effect of hydrophilic inclusions on PDMS crosslinking reaction and its interrelation with mechanical and water sorption properties of cured films. European Polymer Journal 49, 1803–1810 (2013)
Du, P., Lu, H., Zhang, X.: Measuring the Young’s relaxation modulus of PDMS using stress relaxation nanoindentation. In: Materials Research Society Symposium Proceedings, Boston, USA, Materials Research Society (2010)
Duffy, D.C., McDonald, J.C., Schueller, O.J.A., et al.: Rapid prototyping of microfluidic systems in poly(dimethylsiloxane). Analytical Chemistry 70, 4974–4984 (1998)
Chen, J.: Indentation-based methods to assess fracture toughness for thin coatings. Journal of Physics D-Applied Physics 45, 203001 (2012)
Chen, J., Birch, M.A., Bull, S.J.: Nanomechanical characterization of tissue engineered bone grown on titanium alloy in vitro. Journal of Materials Science-Materials in Medicine 21, 277–282 (2010)
Johnson, K.L.: Contact Mechanics. Cambridge University Press, Cambridge (1985)
Chen, J.: Understanding the nanoindentation mechanisms of a microsphere for biomedical applications. Journal of Physics D: Applied Physics 46, 495303 (2013)
Manual A.S.U.s. Hibbitt, Karlsson and Sorensen, Inc. (2012)
Cheng, Y.T., Yang, F.: Obtaining shear relaxation modulus and creep compliance of linear viscoelastic materials from instrumented indentation using axisymmetric indenters of power-law profiles. Journal of Materials Research 24, 3013–3017 (2009)
Oyen, M.L.: Analytical techniques for indentation of viscoelastic materials. Philosophical Magazine 86, 5625–5641 (2006)
Johnson, K.L., Kendall, K., Roberts, A.D.: Surface energy and contact of elastic solids. Proceedings of the Royal Society of London Series A-Mathematical and Physical Sciences 324, 301–313 (1971)
Derjaguin, B.V., Muller, V.M., Toporov, Y.P.: Effect of contact deformations on adhesion of particles. Journal of Colloid and Interface Science 53, 314–326 (1975)
Maugis, D.: On the contact and adhesion of rough surfaces. Journal of Adhesion Science and Technology 10, 161–175 (1996)
Mark, J.: Polymer Data Handbook. Oxford University Press, Oxford (1999)
Armani, D., Liu, C., Aluru, N.: In: Proceedings of IEEE Micro Electro Mechanical System (MEMS)’ 99, Orlando, FL. (IEEE, Piscataway, NJ), 222–227 (1999)
Wang, Z.: Polydimethylsiloxane mechanical properties measured by macroscopic compression and nanoindentation techniques. [Master Dissertation]. University of South Florida, Florida (2011)
Briscoe, B.J., Sebastian, K.S.: The elastoplastic response of polytmethylmethacrylate to indentation. Proc. Roy. Soc. Lond A 452, 439–457 (1996)
Tweedie, C.A., Constantinides, G., Lehman, K.E., et al.: Enhanced stiffness of amorphous polymer surfaces under confinement of localized contact loads. Advanced Materials 19, 2540–2546 (2007)
Tranchida, D., Piccarolo, S., Loos, J., et al.: Mechanical characterization of polymers on a nanometer scale through nanoindentation: A study on pile-up and viscoelasticity. Macromolecules 40, 1259–1267 (2007)
De Silva, R.T., Pasbakhsh, P., Goh, K.L., et al.: Synthesis and characterisation of poly (lactic) acid/halloysite bionanocomposite films. Journal of Composite Materials, (2014) DOI:10.1177/0021998313513046 (in press)
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Chen, J., Wright, K.E. & Birch, M.A. Nanoscale viscoelastic properties and adhesion of polydimethylsiloxane for tissue engineering. Acta Mech Sin 30, 2–6 (2014). https://doi.org/10.1007/s10409-014-0022-0
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DOI: https://doi.org/10.1007/s10409-014-0022-0