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Hemostasis for Severe Hemorrhage with Photocrosslinkable Chitosan Hydrogel and Calcium Alginate

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Abstract

In patients with severe hemorrhage, complications such as shock or death may occur if the patient is not treated appropriately and expeditiously. To create a hemostat kit for severe hemorrhage, ultraviolet light irradiation was applied to photocrosslinkable chitosan hydrogel and calcium alginate. As a hemorrhage model, the femoral arteries and veins of anesthetized rats were cut. Hemodynamics and hematological parameters including red blood cell (RBC) count, hemoglobin concentration, hematocrit, white blood cell (WBC) count, and platelet count, and serum parameters including aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were measured as a marker of hemostasis. In rats for which no procedure was used, death occurred within 30 min. By using the hydrogel hemostat, the survival rate rose to 75% or more. RBC count, hemoglobin, hematocrit, and platelet levels were not significantly changed for 3 days. WBC count increased 1 day after hemostasis. AST and ALT increased 1 day after hemostasis, but it decreased 3 days later. The photocrosslinkable chitosan hydrogel and calcium alginate were biodegraded at 3 and 28 days, respectively, by neutrophils and keratinocyte chemoattractant.

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Acknowledgments

We thank Yaizu Suisankagaku Industry Co., Ltd., for supplying photocrosslinkable chitosan hydrogel. We thank Ms. Ishida for proofreading. We thank Mr. Furukawa and Mr. Kuroda for their fresh advice.

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Authors and Affiliations

  1. Division of Biomedical Engineering, Research Institute, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, 359-8513, Japan

    H. Hattori, Y. Amano & M. Ishihara

  2. Aero-Medical Evacuation Squadron, Japan Air Self-Defense Force, Aichi, Japan

    Y. Nogami

  3. Department of Intensive Care Medicine, National Defense Medical College, Saitama, Japan

    B. Takase

Authors
  1. H. Hattori
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  2. Y. Amano
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  3. Y. Nogami
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  4. B. Takase
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  5. M. Ishihara
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Correspondence to H. Hattori.

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Associate Editor Smadar Cohen oversaw the review of this article.

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Hattori, H., Amano, Y., Nogami, Y. et al. Hemostasis for Severe Hemorrhage with Photocrosslinkable Chitosan Hydrogel and Calcium Alginate. Ann Biomed Eng 38, 3724–3732 (2010). https://doi.org/10.1007/s10439-010-0121-4

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  • DOI: https://doi.org/10.1007/s10439-010-0121-4

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