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Polyelectrolyte Complex Membranes for Prevention of Post-Surgical Adhesions in Neurosurgery

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Abstract

Adhesions are bands of tissue that can form after the surgery and bind together the surrounding tissue in the vicinity of the surgical site. In this work, polyelectrolyte complex (PEC)-based membranes were developed and investigated for their potential application as anti-adhesion barriers after neurosurgery. The adhesion and migration behavior of fibroblast and mixed neuronal cells were also investigated. Fibroblasts cells neither adhered nor migrated onto the PEC material after 5 days in vitro. Similar behaviors were observed for neurons and astrocytes cells. Swelling experiments of these membranes showed that the membranes are extremely hygroscopic and absorb significant amount of water. Membranes containing 70% alginate absorbed the highest amount of water (442 ± 24% of dry wet). PEC content was highest in 50% alginate containing membranes (87.1 ± 6.7%). The membrane’s mechanical properties were attributed to combined contribution of water absorption and PEC content. Drug release profiles were investigated using albumin as a model drug. Membranes containing 70% alginate showed highest initial drug release rate followed by membranes containing 60 and 50% alginate. Membranes were stable and did not dissolve for 1 month in phosphate buffer and lysozyme solutions.

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References

  1. Acheson, A., J. L. Sunshine, and U. Rutishauser. NCAM polysialic acid can regulate both cell–cell and cell-substrate interactions. J. Cell Biol. 114:143, 1991.

    Article  PubMed  CAS  Google Scholar 

  2. Alander, D. H. Gelfoam-induced acute quadriparesis after cervical decompression and fusion. Spine 20:970, 1995.

    Article  PubMed  CAS  Google Scholar 

  3. Brodbeck, W., Y. Nakayama, T. Matsuda, E. Colton, N. Ziats, and J. Anderson. Biomaterial surface chemistry dictates adherent monocyte/macrophage cytokine expression in vitro. Cytokine 18:311–319, 2002.

    Article  PubMed  CAS  Google Scholar 

  4. Burton, C. V., W. Kirkaldy-Willis, K. Yong-Hing, and K. B. Heithoff. Causes of failure of surgery on the lumbar spine. Clin. Orthop. 157:191–199, 1981.

    Google Scholar 

  5. Cabezudo, J. M., A. Lopez, and F. Bacci. Symptomatic root compression by a free fat transplant after hemilaminectomy. J. Neurosurg.: Pediatr. 63:633–635, 1985.

    Google Scholar 

  6. Cemil, B., K. Tun, E. Kaptanoglu, F. Kaymaz, B. Cevirgen, A. Comert, and I. Tekdemir. Use of pimecrolimus to prevent epidural fibrosis in a postlaminectomy rat model. J. Neurosurg.: Pediatr. 11:758–763, 2009.

    Google Scholar 

  7. Chen, Q., Y. Hu, Y. Chen, X. Jiang, and Y. Yang. Microstructure formation and property of chitosan‐poly(acrylic acid) nanoparticles prepared by macromolecular complex. Macromol. Biosci. 5:993–1000, 2005.

    Article  PubMed  CAS  Google Scholar 

  8. De Vasconcelos, C., P. Bezerril, D. Dos Santos, T. Dantas, M. Pereira, and J. Fonseca. Effect of molecular weight and ionic strength on the formation of polyelectrolyte complexes based on poly (methacrylic acid) and chitosan. Biomacromolecules 7:1245–1252, 2006.

    Article  PubMed  Google Scholar 

  9. Einhaus, S. L., J. T. Robertson, F. C. Dohan, Jr., J. R. Wujek, and S. Ahmad. Reduction of peridural fibrosis after lumbar laminotomy and discectomy in dogs by a resorbable gel (ADCON-L). Spine 22:1440, 1997.

    Article  PubMed  CAS  Google Scholar 

  10. Fujimoto, I., J. L. Bruses, and U. Rutishauser. Regulation of cell adhesion by polysialic acid. J. Biol. Chem. 276:31745, 2001.

    Article  PubMed  CAS  Google Scholar 

  11. Hamman, J. H. Chitosan based polyelectrolyte complexes as potential carrier materials in drug delivery systems. Mar. Drugs 8:1305–1322, 2010.

    Article  PubMed  CAS  Google Scholar 

  12. He, Y., M. Revel, and B. Loty. A quantitative model of post-laminectomy scar formation: effects of a nonsteroidal anti-inflammatory drug. Spine 20:557, 1995.

    Article  PubMed  CAS  Google Scholar 

  13. Hieb, L. D. Spontaneous postoperative cerebrospinal fluid leaks following application of anti-adhesion barrier gel: case report and review of the literature. Spine 26:748, 2001.

    Article  PubMed  CAS  Google Scholar 

  14. Ishihara, K., H. Inoue, K. Kurita, and N. Nakabayashi. Selective adhesion of platelets on a polyion complex composed of phospholipid polymers containing sulfonate groups and quarternary ammonium groups. J. Biomed. Mater. Res. 28:1347–1355, 1994.

    Article  PubMed  CAS  Google Scholar 

  15. Ito, T., Y. Iwasaki, T. Narita, K. Akiyoshi, and K. Ishihara. Controlled adhesion of human lymphocytes on electrically charged polymer surface having phosphorylcholine moiety. Sci. Technol. Adv. Mater. 4:99–104, 2003.

    Article  CAS  Google Scholar 

  16. Kacher, D. F., K. Frerichs, J. Pettit, P. K. Campbell, T. Meunch, and A. M. Norbash. DuraSeal magnetic resonance and computed tomography imaging: evaluation in a canine craniotomy model. Neurosurgery 58:ONS140–147, 2006.

    Google Scholar 

  17. LaRocca, H., and I. Macnab. The laminectomy membrane: studies in its evolution, characteristics, effects and prophylaxis in dogs. J. Bone Joint Surg. British 56:545, 1974.

    Google Scholar 

  18. Le, A. X., D. E. Rogers, E. G. Dawson, M. A. Kropf, D. A. De Grange, and R. B. Delamarter. Unrecognized durotomy after lumbar discectomy: a report of four cases associated with the use of ADCON (R)-L. Spine 26:115, 2001.

    Article  PubMed  CAS  Google Scholar 

  19. Li, X., H. Xie, J. Lin, W. Xie, and X. Ma. Characterization and biodegradation of chitosan-alginate polyelectrolyte complexes. Polym. Degrad. Stab. 94:1–6, 2009.

    Article  CAS  Google Scholar 

  20. Li, Z., H. R. Ramay, K. D. Hauch, D. Xiao, and M. Zhang. Chitosan-alginate hybrid scaffolds for bone tissue engineering. Biomaterials 26:3919–3928, 2005.

    Article  PubMed  CAS  Google Scholar 

  21. Martin-Ferrer, S. Failure of autologous fat grafts to prevent postoperative epidural fibrosis in surgery of the lumbar spine. Neurosurgery 24:718, 1989.

    Article  PubMed  CAS  Google Scholar 

  22. Melkerson, M. N. FDA summary of safety and effectiveness data—DuraSeal spine sealant system. PMA P080013, 2009.

  23. Meng, X., F. Tian, J. Yang, C. N. He, N. Xing, and F. Li. Chitosan and alginate polyelectrolyte complex membranes and their properties for wound dressing application. J. Mater. Sci. Mater. Med. 21:1751–1759, 2010.

    Article  PubMed  CAS  Google Scholar 

  24. Moshnikova, A., V. Afanasyev, O. Proussakova, S. Chernyshov, V. Gogvadze, and I. Beletsky. Cytotoxic activity of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide is underlain by DNA interchain cross-linking. Cell. Mol. Life Sci. 63:229–234, 2006.

    Article  PubMed  CAS  Google Scholar 

  25. Nakano, M., H. Matsui, K. Miaki, and H. Tsuji. Postlaminectomy adhesion of the cauda equina: inhibitory effects of anti-inflammatory drugs on cauda equina adhesion in rats. Spine 23:298, 1998.

    Article  PubMed  CAS  Google Scholar 

  26. Ohsumi, H., H. Hirata, T. Nagakura, M. Tsujii, T. Sugimoto, K. Miyamoto, T. Horiuchi, M. Nagao, T. Nakashima, and A. Uchida. Enhancement of perineurial repair and inhibition of nerve adhesion by viscous injectable pure alginate sol. Plast. Reconstr. Surg. 116:823, 2005.

    Article  PubMed  CAS  Google Scholar 

  27. Previtera, M. L., C. G. Langhammer, and B. L. Firestein. Effects of substrate stiffness and cell density on primary hippocampal cultures. J. Biosci. Bioeng. 110:459–470, 2010.

    Article  PubMed  CAS  Google Scholar 

  28. Previtera, M. L., C. G. Langhammer, N. A. Langrana, and B. L. Firestein. Regulation of dendrite arborization by substrate stiffness is mediated by glutamate receptors. Ann. Biomed. Eng. 38:3733–3743, 2010.

    Article  PubMed  Google Scholar 

  29. Previtera, M. L., K. L. Trout, D. Verma, U. Chippada, R. S. Schloss, and N. A. Langrana. Fibroblast morphology on dynamic softening of hydrogels. Ann. Biomed. Eng. 1–12, in-press, 2011.

  30. Rich, A., E. Pearlstein, G. Weissmann, and S. Hoffstein. Cartilage proteoglycans inhibit fibronectin-mediated adhesion. Nature 293:224–226, 1981.

    Google Scholar 

  31. Robertson, J. T., A. L. Meric, F. C. Dohan, Jr., J. B. Schweitzer, J. R. Wujek, and S. Ahmad. The reduction of postlaminectomy peridural fibrosis in rabbits by a carbohydrate polymer. J. Neurosurg. 79:89–95, 1993.

    Article  PubMed  CAS  Google Scholar 

  32. Rosso, F., A. Barbarisi, M. Barbarisi, O. Petillo, S. Margarucci, A. Calarco, and G. Peluso. New polyelectrolyte hydrogels for biomedical applications. Mater. Sci. Eng. C 23:371–376, 2003.

    Article  Google Scholar 

  33. Rutishauser, U. Polysialic acid and the regulation of cell interactions. Curr. Opin. Cell Biol. 8:679–684, 1996.

    Article  PubMed  CAS  Google Scholar 

  34. Rydevik, B., G. Lundborg, and C. Nordborg. Intraneural tissue reactions induced by internal neurolysis. J. Plast. Reconstr. Surg. Hand Surg. 10:3–8, 1976.

    CAS  Google Scholar 

  35. Sandoval, M. A., and D. Hernandez-Vaquero. Preventing peridural fibrosis with nonsteroidal anti-inflammatory drugs. Eur. Spine J. 17:451–455, 2008.

    Article  PubMed  Google Scholar 

  36. Schatz, C., J. M. Lucas, C. Viton, A. Domard, C. Pichot, and T. Delair. Formation and properties of positively charged colloids based on polyelectrolyte complexes of biopolymers. Langmuir 20:7766–7778, 2004.

    Article  PubMed  CAS  Google Scholar 

  37. Snow, D. M., V. Lemmon, D. A. Carrino, A. I. Caplan, and J. Silver. Sulfated proteoglycans in astroglial barriers inhibit neurite outgrowth in vitro. Exp. Neurol. 109:111–130, 1990.

    Article  PubMed  CAS  Google Scholar 

  38. Songer, M. N., L. Ghosh, and D. L. Spencer. Effects of sodium hyaluronate on peridural fibrosis after lumbar laminotomy and discectomy. Spine 15:550, 1990.

    Article  PubMed  CAS  Google Scholar 

  39. Songer, M. N., W. Rauschning, E. W. Carson, and S. M. Pandit. Analysis of peridural scar formation and its prevention after lumbar laminotomy and discectomy in dogs. Spine 20:571, 1995.

    Article  PubMed  CAS  Google Scholar 

  40. Stütz, N. M., A. Gohritz, A. Novotny, U. Falkenberg, U. Lanz, and J. van Schoonhoven. Clinical and electrophysiological comparison of different methods of soft tissue coverage of the median nerve in recurrent carpal tunnel syndrome. Neurosurgery 62:194, 2008.

    Article  PubMed  Google Scholar 

  41. Tatsui, C. E., G. Martinez, X. Li, P. Pattany, and A. D. Levi. Evaluation of DuraGen in preventing peridural fibrosis in rabbits. J. Neurosurg. Spine 4:51–59, 2006.

    Article  PubMed  Google Scholar 

  42. Verma, D., M. S. Desai, N. Kulkarni, and N. Langrana. Characterization of surface charge and mechanical properties of chitosan/alginate based biomaterials. Mater. Sci. Eng. C 31:1741–1747, 2011.

    Article  CAS  Google Scholar 

  43. Wang, D., Y. Wen, X. Lan, and H. Li. Experimental study on bone marrow mesenchymal stem cells seeded in chitosan-alginate scaffolds for repairing spinal cord injury. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 24:190–196, 2010.

    PubMed  CAS  Google Scholar 

  44. Xu, J., S. E. Varitimidis, K. J. Fisher, M. M. Tomaino, and D. G. Sotereanos. The effect of wrapping scarred nerves with autogenous vein graft to treat recurrent chronic nerve compression. J. Hand Surg. 25:93–103, 2000.

    Article  Google Scholar 

  45. Yong-Hing, K., J. Reilly, V. De Korompay, and W. Kirkaldy-Willis. Prevention of nerve root adhesions after laminectomy. Spine 5:59, 1980.

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This work was support by NJSCR #08-3080-SCR-E-0. We would also like to thank Drs. Wise Young and Dongming Sun for their scientific advisement. We would also like to acknowledge Namrata Kulkarni and Vikas Shah for their efforts.

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Authors and Affiliations

  1. Department of Biomedical Engineering, Rutgers University, Piscataway, NJ, USA

    Devendra Verma, Michelle L. Previtera, Rene Schloss & Noshir Langrana

  2. Department of Mechanical and Aerospace Engineering, Rutgers University, Piscataway, NJ, USA

    Noshir Langrana

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  1. Devendra Verma
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  2. Michelle L. Previtera
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  4. Noshir Langrana
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Correspondence to Devendra Verma.

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Associate Editor Scott I. Simon oversaw the review of this article.

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Verma, D., Previtera, M.L., Schloss, R. et al. Polyelectrolyte Complex Membranes for Prevention of Post-Surgical Adhesions in Neurosurgery. Ann Biomed Eng 40, 1949–1960 (2012). https://doi.org/10.1007/s10439-012-0564-x

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  • DOI: https://doi.org/10.1007/s10439-012-0564-x

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