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Use of Flow, Electrical, and Mechanical Stimulation to Promote Engineering of Striated Muscles

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Abstract

The field of tissue engineering involves design of high-fidelity tissue substitutes for predictive experimental assays in vitro and cell-based regenerative therapies in vivo. Design of striated muscle tissues, such as cardiac and skeletal muscle, has been particularly challenging due to a high metabolic demand and complex cellular organization and electromechanical function of the native tissues. Successful engineering of highly functional striated muscles may thus require creation of biomimetic culture conditions involving medium perfusion, electrical and mechanical stimulation. When optimized, these external cues are expected to synergistically and dynamically activate important intracellular signaling pathways leading to accelerated muscle growth and development. This review will discuss the use of different types of tissue culture bioreactors aimed at providing conditions for enhanced structural and functional maturation of engineered striated muscles.

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Acknoweldgments

This work was supported by NIH-NIAMS Grants AR055226 and AR065873, NIH-NHLBI Grant HL104326, and NIH Common Fund for the Microphysiological Systems Initiative Grant UH2TR000505 to N.B. The content of this manuscript is solely the responsibility of the authors and does not necessarily represent the official views of the NIH.

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  1. Department of Biomedical Engineering, Duke University, 3000 Science Drive, Hudson Hall-Engineering, Room 136, P.O. Box 90281, Durham, NC, 27708, USA

    Swathi Rangarajan, Lauran Madden & Nenad Bursac

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  1. Swathi Rangarajan
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  2. Lauran Madden
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Correspondence to Nenad Bursac.

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Associate Editor Tzung Hsiai oversaw the review of this article.

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Rangarajan, S., Madden, L. & Bursac, N. Use of Flow, Electrical, and Mechanical Stimulation to Promote Engineering of Striated Muscles. Ann Biomed Eng 42, 1391–1405 (2014). https://doi.org/10.1007/s10439-013-0966-4

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